Comprehensive analysis of mRNA Transcriptome Profiles in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3 DOI
Hanwei Jiao,

Chi Meng,

Fengyuan Jiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Background Porcine hepatitis E is a zoonotic infectious disease caused by swine virus (HEV), open reading frames 3 an important virulence protein of porcine HEV, which plays role in the release viral particles and host innate immune response, regulation autophagy apoptosis, etc., but its main function pathogenic mechanism are not perfect current research. Results In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They mainly involved 6 pathways: cellular response to unfolded protein, Inflammatory cytokine activity, TNF signaling pathway, Influenza A, Pathways cancer. Conclusions The differential verified, laid genetic foundation physiological HEV ORF3

Language: Английский

Infectious hepatitis E virus is associated with the mature sperm head DOI Creative Commons
Kush Kumar Yadav, Patricia A. Boley, Thamonpan Laocharoensuk

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(5), P. e1012240 - e1012240

Published: May 20, 2024

Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide. HEV associated pregnancy mortality has been reported as up to 30% in humans. Recent findings suggest may elicit effects directly reproductive system with protein found testis, RNA semen, and replication occurring placental cell types. Using a natural host model for infection, pigs, we demonstrate infectious within mature spermatozoa altered sperm viability from infected pigs. isolated remained suggesting potential transmission route via sexual partners. Our that should be explored possible sexually transmittable disease. propose infection routes outside oral intravenous need considered their contribute higher infections when involved disease general.

Language: Английский

Citations

6
Effect of Hepatitis E Virus on the Male Reproductive System: A Review of Current Evidence DOI Creative Commons
Ahmed A. Kotb, Mohamed A. El‐Mokhtar, Ibrahim M. Sayed

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(1), P. 66 - 66

Published: Jan. 5, 2025

Hepatitis E Virus (HEV) is a globally widespread pathogen that causes acute hepatitis infection. Beyond hepatic pathogenesis, HEV has been proven to cause several extrahepatic manifestations, such as neurological, renal, and hematological manifestations. It was also associated with mortality in pregnant females. Several studies have investigated the impact of on male reproductive system; however, available data are limited conflicting. Assessment patients’ ejaculates/semen samples revealed particles excreted these fluids cases chronic infection but not The infectious vivo animal models vitro cell culture. However, effect infertility confirmed. One study including human showed genotype 4 Studies pigs, gerbils, mice caused distortion testes, damage blood–testis barrier, induction inflammatory responses leading abnormalities sperm. excretion semen raises concerns about transmission via sexual transmission. all do confirm through intercourse. This review aims summarize critically assess investigating influence different genotypes system, providing insights into whether contributes impairment men.

Language: Английский

Citations

0
Descriptive Comparative Transcriptomic Analysis of Genotype IV SHEV ORF3-Expressing HepG2 Cells DOI Creative Commons
Hanwei Jiao,

Chi Meng,

Fengyuan Jiao

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(2), P. 412 - 412

Published: Feb. 13, 2025

Background: Swine hepatitis E (HEV) is a zoonotic infectious disease caused by the swine virus (SHEV). Open reading frame 3 (ORF3) key virulence factor in HEV, playing crucial role release of viral particles, modulation host innate immune response, and regulation autophagy apoptosis, etc. However, its main function pathogenic mechanism remain incompletely understood. Results: In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent protein (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They are mainly involved six pathways: cellular response to unfolded protein, inflammatory cytokine activity, TNF signaling pathway, influenza A, pathways cancer. comparative analysis transcriptome mRNA expression profiles lncRNA results showed that mRNAs GPX1, MDM4, CLDN 39 transcripts overlapped have been identified. Conclusions: Eight differential genes, HSPA1A, HSPA1B, PLD3, RELA, GPI, SAMHD1, RPS6KA4, PIK3CB, verified. Comparing analyzing two sequencing methods indicated identified SHEV ORF3-expressing cells, which has laid genetic foundation physiological ORF3.

Language: Английский

Citations

0
The gamma-glutamyl transpeptidase to platelet ratio: a good noninvasive biomarker for predicting for the occurrence and prognosis of patients with hepatitis E virus-related acute liver failure DOI Creative Commons
Shuai Zhu, Jun Yuan,

Feng Ju

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: March 24, 2025

Background The relationship between GPR and hepatitis E has not been reported. This study evaluates the levels in AHE patients HEV-ALF explores role of prognosis patients, offering new strategies methods for diagnosis patients. Methods Serum samples were collected from 206 217 admitted to First Affiliated Hospital Zhejiang University School Medicine, Suzhou Yongding Hospital, Nanjing Medical January 1, 2017, November 30, 2024. Results level group was significantly higher than ( p < 0.001). OPLS-DA analysis revealed that INR, TBIL, PLT, GPR, TCH, ALT, AFP, TP, ALB, AST major influential factors occurrence HEV-ALF. showed good diagnostic performance with an AUC 0.701. DCA further supported utility across a range threshold probabilities. Regarding prediction 30-day mortality, non-survival compared survival highlighted as most factor predicting mortality. demonstrated 0.703. results also indicated GPR’s strong decision-making ability mortality Conclusion is highly expressed closely related their prognosis.

Language: Английский

Citations

0
A porcine chronic hepatitis E virus (HEV) infection model exhibits HEV replication in male accessory reproductive glands and immune-mediated reproductive damage DOI
Kush Kumar Yadav, Patricia A. Boley, Thamonpan Laocharoensuk

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 10, 2024

Abstract Hepatitis E virus (HEV) is an expanding zoonotic viral disease threat. Although HEV causes acute hepatitis, it increasingly being recognized as a systemic pathogen with detection and damage in extrahepatic tissues. The presence of RNA the semen chronically infected human patients absence viremia fecal shedding sperm head underscores need to understand interaction within male reproduction system. Male accessory glands secrete biofluids necessary for nourishment neutralize acidity vagina. role dissemination persistence infection have not been studied. Using immunosuppressed pig model chronic infection, we demonstrate infectious mature cells altering motility morphology. isolated from remained hepatoma cells. Spermatic fluid contained lower titers than pigs highlighting that themselves can associate virus. Evaluation demonstrated replication, infiltration CD45 leukocytes, apoptosis associated infection. A decrease serum testosterone levels was evident pigs. Even though titer seen feces infected, ribavirin treated pigs, high particles concern. Our findings necessitate further studies defining mechanism cell invasion by HEV, length survival during risk sexual transmission both phases Author Summary virus, leading cause humans. Recent advances field diseases HEV. More recent revealed vagina, Sertoli cells, ejaculate humans, We sex may infections. utilized established study loads highlights importance testis, immune privileged site, maintenance New evaluate mechanisms which associates fractions, consideration testes potential reservoir are necessary.

Language: Английский

Citations

0
Comprehensive analysis of mRNA Transcriptome Profiles in HepG2 Cells Expressing Genotype IV Swine Hepatitis E Virus ORF3 DOI
Hanwei Jiao,

Chi Meng,

Fengyuan Jiao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 9, 2024

Abstract Background Porcine hepatitis E is a zoonotic infectious disease caused by swine virus (HEV), open reading frames 3 an important virulence protein of porcine HEV, which plays role in the release viral particles and host innate immune response, regulation autophagy apoptosis, etc., but its main function pathogenic mechanism are not perfect current research. Results In our study, adenoviruses ADV4-ORF3 ADV4-GFP were successfully constructed mediated overexpression enhanced green fluorescent (EGFP)-ORF3 EGFP HepG2 cells. A total 217 differentially expressed messenger RNAs (mRNAs) screened high-throughput sequencing, 27 statistically significant genes for further quantitative real-time reverse transcription (qRT-PCR) verification functional enrichment (Gene Ontology [GO] Kyoto Encyclopedia Genes Genomes [KEGG]). They mainly involved 6 pathways: cellular response to unfolded protein, Inflammatory cytokine activity, TNF signaling pathway, Influenza A, Pathways cancer. Conclusions The differential verified, laid genetic foundation physiological HEV ORF3

Language: Английский

Citations

0