Highly selective and sensitive molecularly imprinted sensors for the electrochemical assay of quercetin in methanol extracts of Rubus sanctus and Fragaria vesca

Talanta, Journal Year: 2024, Volume and Issue: 273, P. 125883 - 125883

Published: March 12, 2024

Language: Английский

---

Optimized Hesperidin-Loaded Lipid Nanoparticles with Tea Tree Oil for Enhanced Wound Healing: Formulation, Characterization, and Evaluation

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(3), P. 290 - 290

Published: Feb. 20, 2025

Objectives: This study aimed to develop hesperidin solid lipid nanoparticles (HESP-SLNs) enhance their stability, solubility, and sustained release for wound healing; further enhancement was achieved through prepared nanostructured carriers (HESP-NLCs) using Tea Tree Oil (TTO) explore synergistic efficacy. Methods: A factorial design of 24 trials established evaluate the influence type (X1), conc (%) (X2), surfactant (X3), sonication amplitude (X4) HESP-SLNs on particle size (nm) (Y1), polydispersibility index (Y2), zeta potential (Y3), encapsulation efficiency (Y4). The optimized formula selected utilizing Design Expert® software version 13, which additionally enhanced by preparing TTO-loaded HESP-NLCs. In vitro release, Raman spectroscopy, transmission electron microscopy were carried out both nanoparticles. Cytotoxicity, in vivo wound-healing assessments, skin irritancy tests performed performance TTO-incorporated HESP-NLCs compared HESP-SLNs. Results: demonstrated PS (280 ± 1.35 nm), ZP (−39.4 0.92 mV), PDI (0.239 0.012), EE% (88.2 2.09%). NLCs Q6% (95.14%) vs. (79.69%), SLNs showed superior antimicrobial Both exhibited spherical morphology compatibility between HESP excipients. highest closure percentage, supported histological analysis inflammatory biomarker outcomes. Cytotoxicity evaluation 87% cell viability untreated HSF cells, test confirmed safety NLCs. Conclusions: are promising candidates exhibiting capabilities, making them a therapeutic option cutaneous management.

Language: Английский

---

Eco-friendly extraction of hesperidin from citrus peels: a comparative study of Soxhlet, ultrasound-assisted, and microwave-assisted methods for improved yield and antioxidant properties

International Journal of Environmental Health Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: March 26, 2025

This study compares Soxhlet extraction, ultrasound-assisted extraction (UAE), and microwave-assisted (MAE) for isolating hesperidin from citrus peels. UAE achieved the highest yield (89.7%) demonstrated favorable kinetic parameters (rate constant: 0.96 h-1, half-life: 0.72 h), highlighting its efficiency as an eco-friendly alternative. The extract also exhibited total phenolic flavonoid contents, maximizing bioactive compound recovery. While MAE showed superior antioxidant activity in DPPH (IC₅₀: 31.49 µg/mg DE) ABTS 73.38 assays, HPLC analysis confirmed a purity of 89.7%, with yielding comparable results. These findings underscore UAE's potential enhancing while promoting sustainability through waste valorization. research provides valuable insights into optimizing techniques compounds supporting their industrial applications.

Language: Английский

---

Bioflavonoids: A Detailed Take on Their Extraction Methods, Stability, and Applications

Published: Jan. 1, 2025

Language: Английский

---

Citrus Flavonoid Hesperetin Inhibits α-Synuclein Fibrillogenesis, Disrupts Mature Fibrils, and Reduces Their Cytotoxicity: In Vitro and In Vivo Studies

Journal of Agricultural and Food Chemistry, Journal Year: 2023, Volume and Issue: 71(43), P. 16174 - 16183

Published: Oct. 23, 2023

Misfolding and subsequent fibrillogenesis of α-synuclein (αSN) significantly influence the development Parkinson's disease (PD). This study reports inhibitory effect citrus flavonoid hesperetin (Hst) on αSN fibrillation. Based thioflavin T fluorometry atomic force microscopy studies, Hst inhibited fibrillation by interfering with initial nucleation slowing elongation rate. Furthermore, was concentration-dependent a half-maximal concentration 24.4 μM. Cytotoxicity experiments showed that 100 μM reduced cytotoxicity aggregates maintained 98.4% cell activity. In addition, disassembled preprepared fibrils into smaller less-toxic aggregates. Excitingly, supplementation accumulation 36.3% in NL5901 restored amyloid-induced reduction lipid abundance, extending mean lifespan to 23 d. These findings could support use as dietary supplement regulate prevent PD.

Language: Английский

---

The fundamental efficacy of hesperidin-loaded/chitosan-coated hybrid nanoparticles as a prospective regimen in wound healing amendment: In vitro and in vivo comprehensive study

Journal of Drug Delivery Science and Technology, Journal Year: 2023, Volume and Issue: 92, P. 105302 - 105302

Published: Dec. 25, 2023

Language: Английский

---

Supercritical carbon dioxide and ethanol-assisted extraction of bioactive compounds from Bourbon, Catimor, and Caturra coffee pulp for maximized antioxidant and therapeutic properties

Future Foods, Journal Year: 2024, Volume and Issue: 9, P. 100381 - 100381

Published: May 21, 2024

Coffee pulp waste contains valuable bioactive components that are often discarded, leading to environmental impact. The optimum level of supercritical (50% CO2: ethanol) extraction (SFE) condition revealed the highest yield% and antioxidant activity assayed by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) in coffee peel compared hot water (85°C), 80% ethanol. Additionally, optimized SFE at 60°C, 300 Bar, 60 min showed yield (p>0.05), Bourbon, Caturra Catimor extracts (CPE). Cell-based experiments unveiled fascinating biological effects CPE. Cytotoxicity both RAW 264.7 HepG-2 cells, indicating their potential trigger apoptosis, all while demonstrating anti-inflammatory properties. Among different varieties, consistently exhibited activity. Western blot analysis further elucidated extracts' capacity influence critical cellular markers linked oxidative stress, inflammation, responses, with unaltered NF-kB expression implying a targeted impact on specific pathways. Therefore, using 50% ethanol ratios could extract maximum phytochemicals impart excellent functional properties as ingredient for food fortification.

Language: Английский

---

Hesperidin, a Potential Antiviral Agent against SARS-CoV-2: The Influence of Citrus Consumption on COVID-19 Incidence and Severity in China

Medicina, Journal Year: 2024, Volume and Issue: 60(6), P. 892 - 892

Published: May 28, 2024

This review examines hesperidin, a citrus bioflavonoid, as potential antiviral agent against SARS-CoV-2. The COVID-19 pandemic has demanded an urgent need to search for effective compounds, including those of natural origin, such hesperidin. provides comprehensive analysis the chemical properties, bioavailability and mechanisms particularly its efficacy A databases, PubMedPico, Scopus Web Science, was conducted using specific keywords criteria in accordance with PRISMA (Re-porting Items Systematic Reviews Meta-Analysis) guidelines between 2020 2024. Of 207 articles, 37 were selected review. key aspect is correlation vitro, silico clinical studies on effects hesperidin epidemiological data consumption China during 2020–2024. importance integrating laboratory findings actual patterns better understand role mitigating highlighted, attempt made analyze examine association juice source incidence severity example. identifies consistencies discrepancies experimental data, highlighting correlate two fields Challenges limitations interpreting results future research perspectives this area are discussed. aim bridge gap evidence contribute understanding their correlation.

Language: Английский

---

Quality assessment and Q‐markers discovery in Citri Sarcodactylis Fructus by integrating serum pharmacochemistry and network pharmacology

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: 35(5), P. 1017 - 1035

Published: Feb. 18, 2024

Citri Sarcodactylis Fructus (CSF), a common fruit and traditional Chinese medicine (TCM), has been hindered in its further development research owing to the lack of comprehensive specific quality evaluation standards.

Language: Английский

---

Safety evaluation of excessive intake of Hesperetin-7-Glucoside-β-Cyclodextrin Inclusion Complex in Healthy Japanese Subjects

Functional Foods in Health and Disease, Journal Year: 2024, Volume and Issue: 14(3), P. 157 - 168

Published: March 4, 2024

Background: Hesperidin, a flavonoid glycoside, is widely found in the peels and rinds of citrus fruits, offering various physiological benefits. However, its effectiveness hindered by challenges related to insolubility low bioavailability. To overcome these obstacles, we developed Hesperetin-7-glucoside -β-cyclodextrin inclusion complex (HCD). This demonstrates superior solubility bioavailability compared hesperidin. In previous study where participants consumed 300 mg/day HCD for 12 weeks, there was notable improvement endothelial dysfunction. Importantly, no significant adverse clinical events were reported during this period. Objective: evaluate safety excessive intake Healthy Japanese subjects. Methods: Fourteen healthy male female volunteers (with mean age 39.1±9.1) participated trial. Subjects took 1500 HCD, which five times dosage 4 consecutive weeks. Physical examination, blood tests, uric tests performed Results: Results demonstrated differences at 2, weeks baseline 0 with mg (equivalent 195 HPTG) supplementation Conclusions: It has been that are concerns when consuming daily, continuously Keywords: Safety, Bioavailability, Clinical trials, Food, Foods Function Claims, Overdose supplementation, Cyclodextrin Trial registration: UMIN-CTR (Trial ID: UMIN000051960) Foundation: Taiyo Kagaku Co. Ltd.

Language: Английский

---