Microbial transformation of sewage sludge to biolipid-based fuel using potential oleaginous bacteria Streptomyces sp. DOI Creative Commons

Sana Akbar,

Muzammil Anjum, Samia Qadeer

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 14, 2025

Introduction The high proportion of sludge generation worldwide has sparked interest in utilizing it for alternative purposes. Among different potential applications, using as a substrate oleaginous bacteria is relatively novel approach. study was conducted to harness Streptomyces sp. produce bio-lipids and their further processing biofuel through transesterification. Methods Sewage obtained from the I-9 treatment plant, Islamabad; after initial characterization unprocessed optimized viz.: incubation time (24–96 h), inoculation rate (5–15%), pH levels (4–9), temperature (25–40°C), agitation (0–250 RPM), nitrogen sources (yeast, urea, ammonium chloride, nitrate), carbon (glucose, sucrose, starch, dextrose). qualitative analysis stored performed Fourier-transform infrared spectroscopy (FTIR) Gas Chromatography Mass Spectrometry (GC–MS). Results discussion maximum reactor performance achieved with 40% lipid accumulation (gravimetric basis) dry cell biomass results indicated presence C-H (Alkane), additional phenolic alcoholic bonds FTIR, whereas GC–MS palmitic acid oleic most recurring compounds. This highlights strong biolipid based fuel production substrate. contents extract (i.e., bio-lipids) were successfully transesterified biofuels lipids. findings that use potentially provides dual benefit reducing organic loading along under conditions.

Language: Английский

Microbial transformation of sewage sludge to biolipid-based fuel using potential oleaginous bacteria Streptomyces sp. DOI Creative Commons

Sana Akbar,

Muzammil Anjum, Samia Qadeer

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: May 14, 2025

Introduction The high proportion of sludge generation worldwide has sparked interest in utilizing it for alternative purposes. Among different potential applications, using as a substrate oleaginous bacteria is relatively novel approach. study was conducted to harness Streptomyces sp. produce bio-lipids and their further processing biofuel through transesterification. Methods Sewage obtained from the I-9 treatment plant, Islamabad; after initial characterization unprocessed optimized viz.: incubation time (24–96 h), inoculation rate (5–15%), pH levels (4–9), temperature (25–40°C), agitation (0–250 RPM), nitrogen sources (yeast, urea, ammonium chloride, nitrate), carbon (glucose, sucrose, starch, dextrose). qualitative analysis stored performed Fourier-transform infrared spectroscopy (FTIR) Gas Chromatography Mass Spectrometry (GC–MS). Results discussion maximum reactor performance achieved with 40% lipid accumulation (gravimetric basis) dry cell biomass results indicated presence C-H (Alkane), additional phenolic alcoholic bonds FTIR, whereas GC–MS palmitic acid oleic most recurring compounds. This highlights strong biolipid based fuel production substrate. contents extract (i.e., bio-lipids) were successfully transesterified biofuels lipids. findings that use potentially provides dual benefit reducing organic loading along under conditions.

Language: Английский

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