Particulate matter 10 exposure affects intestinal functionality in both inflamed 2D intestinal epithelial cell and 3D intestinal organoid models DOI Creative Commons
Ye Seul Son, Naeun Son,

Won Dong Yu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 26, 2023

Background A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing GI epithelial cells to function less efficiently, leading inflammation and an imbalance in gut microbiome. PM10 may, however, act as exacerbation factor patients with inflamed intestinal epithelium, which is associated inflammatory bowel disease. Objective The purpose this study was dissect pathology mechanism exposure intestines. Methods In study, we established chronically epithelium models utilizing two-dimensional (2D) human (hIECs) 3D organoids (hIOs), mimic vivo cellular diversity function, order examine deleterious effects intestine-like vitro models. Results Inflamed 2D hIECs hIOs exhibited pathological features, such inflammation, decreased markers, defective barrier function. addition, found induced a more severe disturbance peptide uptake than control cells. This due fact it interferes calcium signaling, protein digestion, absorption pathways. findings demonstrate PM10-induced alterations contribute disorders caused by intestine. Conclusions According our findings, hIEC hIO could be powerful platforms for evaluation causal relationship between PM abnormal functions.

Language: Английский

Myosin 2 – A general contractor for the cytoskeleton DOI

Joseph J. Tidei,

Patrick W. Oakes, Jordan R. Beach

et al.

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 94, P. 102522 - 102522

Published: May 3, 2025

Language: Английский

Citations

0
Tight junctions regulate lumen morphology via hydrostatic pressure and junctional tension DOI Creative Commons
Markus Mukenhirn,

Chen-Ho Wang,

Tristan Guyomar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 23, 2023

Summary Formation of fluid filled lumen by epithelial tissues is a fundamental process for organ development. How cells regulate the hydraulic and cortical forces to control morphology not completely understood. Here, we quantified mechanical role tight junctions in formation using genetically modified MDCKII cysts. We found that paracellular ion barrier formed claudin receptors required inflation lumen. However, depletion zonula occludens scaffold resulted collapse folding apical membranes. Combining quantitative measurements perturbations hydrostatic pressure junctional tension with modelling, were able predict morphologies from pressure-tension force balance. MDCK tissue junction promotes spherical decreasing via inhibition myosin. In addition, surface area largely uncoupled volume changes, suggesting excess contributes opening low-pressure regime. Overall, our findings provide understanding how use modulate shape.

Language: Английский

Citations

9
ZnUMBA – a live imaging method to detect local barrier breaches DOI Creative Commons
Tomohito Higashi, Rachel E. Stephenson, Cornelia Schwayer

et al.

Journal of Cell Science, Journal Year: 2023, Volume and Issue: 136(15)

Published: July 18, 2023

Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage the monolayer. Although these methods measure changes in global function, they lack sensitivity needed to detect local transient breaches, do not reveal location of leaks. Therefore, we previously developed method that named zinc-based ultrasensitive microscopic assay (ZnUMBA), overcomes limitations, allowing for detection tight junction leaks with high spatiotemporal resolution. Here, present expanded applications ZnUMBA. ZnUMBA can be used Xenopus embryos dynamics restoration actin accumulation following laser injury. also effectively utilized developing zebrafish as well cultured monolayers Madin-Darby canine kidney (MDCK) II epithelial cells. powerful flexible that, minimal optimization, applied multiple systems dynamic precision.

Language: Английский

Citations

8
The dynamics and biophysics of shape formation: Common themes in plant and animal morphogenesis DOI Creative Commons
Isabella Burda, Adam C. Martin, Adrienne Roeder

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 58(24), P. 2850 - 2866

Published: Dec. 1, 2023

Language: Английский

Citations

8
Particulate matter 10 exposure affects intestinal functionality in both inflamed 2D intestinal epithelial cell and 3D intestinal organoid models DOI Creative Commons
Ye Seul Son, Naeun Son,

Won Dong Yu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: June 26, 2023

Background A growing body of evidence suggests that particulate matter (PM10) enters the gastrointestinal (GI) tract directly, causing GI epithelial cells to function less efficiently, leading inflammation and an imbalance in gut microbiome. PM10 may, however, act as exacerbation factor patients with inflamed intestinal epithelium, which is associated inflammatory bowel disease. Objective The purpose this study was dissect pathology mechanism exposure intestines. Methods In study, we established chronically epithelium models utilizing two-dimensional (2D) human (hIECs) 3D organoids (hIOs), mimic vivo cellular diversity function, order examine deleterious effects intestine-like vitro models. Results Inflamed 2D hIECs hIOs exhibited pathological features, such inflammation, decreased markers, defective barrier function. addition, found induced a more severe disturbance peptide uptake than control cells. This due fact it interferes calcium signaling, protein digestion, absorption pathways. findings demonstrate PM10-induced alterations contribute disorders caused by intestine. Conclusions According our findings, hIEC hIO could be powerful platforms for evaluation causal relationship between PM abnormal functions.

Language: Английский

Citations

8