Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone Regeneration

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Diabetes mellitus is a metabolic disorder associated with an increased risk of fractures and delayed fracture healing, leading to higher prevalence bone defects. Recent advancements in strategies aim at regulating immune responses enhancing neurovascularization have not met expectations. This study demonstrates that silicon‐based strategy significantly enhances vascularization innervation, thereby optimizing the repair diabetic Silicon improves mitochondrial function modulates fission dynamics macrophages via Drp1‐Mff signaling pathway. Subsequently, functional mitochondria are transferred from endothelial neuronal cells through microvesicles, providing protective mechanism for blood vessels peripheral nerves during early wound healing. On this basis, optimized combining silicified collagen scaffold Drp1‐Fis1 interaction inhibitor used further regulate enhance trafficking into stressed receptor cells. In mice critical‐sized calvarial defects, treatment promotes vessel formation, nerve growth, mineralized tissue development. These findings provide therapeutic insights role silicon promoting regeneration highlight importance intercellular communication conditions.

Language: Английский

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Horizontal transfer of nuclear DNA in transmissible cancer

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(18)

Published: April 22, 2025

Horizontal transfer of nuclear DNA between cells host and cancer is a potential source adaptive variation in cells. An understanding the frequency significance this process naturally occurring tumors is, however, lacking. We screened for phenomenon transmissible cancers dogs Tasmanian devils found an instance canine venereal tumor (CTVT). This involved introduction 15-megabase dicentric genetic element, composed 11 fragments six chromosomes, to CTVT sublineage Asia around 2,000 y ago. The element forms short arm small submetacentric chromosome derives from dog with ancestry associated ancient Middle East. introduced fragment transcriptionally active has adopted expression profile CTVT. Its features suggest that it may derive engulfed apoptotic body. Our findings indicate horizontal gene transfer, although likely rare event evolution, provides viable mechanism acquisition material genomes.

Language: Английский

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Clinical Approaches for Mitochondrial Diseases

Cells, Journal Year: 2023, Volume and Issue: 12(20), P. 2494 - 2494

Published: Oct. 20, 2023

Mitochondria are subcontractors dedicated to energy production within cells. In human mitochondria, almost all mitochondrial proteins originate from the nucleus, except for 13 subunit that make up crucial system required perform 'oxidative phosphorylation (OX PHOS)', which expressed by mitochondria's self-contained DNA. Mitochondrial DNA (mtDNA) also encodes 2 rRNA and 22 tRNA species. replicates autonomously, independent of its heredity follows a non-Mendelian pattern, exclusively passing mother children. Numerous studies have identified mtDNA mutation-related genetic diseases. The consequences various types mutations, including insertions, deletions, single base-pair studied reveal their relationship Most diseases exhibit fatal symptoms, leading ongoing therapeutic research with diverse approaches such as stimulating defective OXPHOS system, replacement, allotropic expression enzymes. This review provides detailed information on two topics: (1) caused (2) mechanisms current treatments clinical trials.

Language: Английский

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Exploring the limitations of mitochondrial dye as a genuine horizontal mitochondrial transfer surrogate

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: March 7, 2024

Abstract Rosamine-based mitochondrial dyes, such as Mitotracker Red, have commonly been employed to visualize localization within cells due their preferential accumulation in organelles with membrane potential. Consequently, Red has often served a surrogate indicator for tracking movement between neighboring cells. However, it is important note that the presence of potential cell and other may lead non-specific partial enrichment locations than mitochondria. This study comprehensively investigates reliability dye marker studying horizontal transfer (HMT). By meticulous replicating previous experiments comparing efficiency mito-targeted GFP, our findings confirm HMT occurs at significantly lower previously indicated by dye. Subsequent involving mitochondria-deficient robustly demonstrates non-specificity We advocate thorough reevaluation existing literature this field propose exploration alternative techniques enhance investigation HMT. addressing these pivotal aspects, we can advance understanding cellular dynamics pave way future explorations captivating field.

Language: Английский

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The adaptor protein Miro1 modulates horizontal transfer of mitochondria in mouse melanoma models

Cell Reports, Journal Year: 2025, Volume and Issue: 44(1), P. 115154 - 115154

Published: Jan. 1, 2025

Recent research has shown that mtDNA-deficient cancer cells (ρ0 cells) acquire mitochondria from tumor stromal to restore respiration, facilitating formation. We investigated the role of Miro1, an adaptor protein involved in movement along microtubules, this phenomenon. Inducible Miro1 knockout (Miro1KO) mice markedly delayed formation after grafting ρ0 cells. Miro1KO with fluorescently labeled revealed delay was due hindered mitochondrial transfer grafted B16 cells, which impeded recovery respiration and growth. led perinuclear accumulation impaired mobility network. In vitro experiments decreased association compromising via tunneling nanotubes (TNTs) mesenchymal Here we show horizontal mouse melanoma models vivo its involvement TNTs.

Language: Английский

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