EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases DOI
Ipsita Nandi, Rachana Pattani Ramachandran, Deborah E. Shalev

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 29, 2024

Abstract Enteropathogenic E. coli (EPEC) is a Gram-negative bacterial pathogen that causes persistent diarrhea. Upon attachment to the apical plasma membrane of intestinal epithelium, translocates virulent proteins called effectors into infected cells. These hijack numerous host processes for pathogen’s benefit. Therefore, studying mechanisms underlying their action crucial better understanding disease. We show translocated EspH interacts with multiple Rab GTPases. AlphaFold predictions and site-directed mutagenesis identified glutamic acid lysine at positions 37 41 as interacting residues. Mutating these sites abolished ability inhibit Akt mTORC1 signaling, lysosomal exocytosis, invasion. Knocking out endogenous Rab8a gene expression highlighted involvement in Akt/mTORC1 signaling exocytosis. A phosphoinositide binding domain critical tyrosine was EspH. localization infection its capacity interact Rabs. Our data suggest novel EspH-dependent elicit immune trafficking during EPEC infection.

Language: Английский

Mitochondrial quality control and stress signaling pathways in the pathophysiology of cardio-renal diseases DOI Creative Commons
Isabel Amador‐Martínez, Ana Karina Aranda‐Rivera,

Mauricio Raziel Martínez-Castañeda

et al.

Mitochondrion, Journal Year: 2025, Volume and Issue: unknown, P. 102040 - 102040

Published: April 1, 2025

Mitochondria are essential organelles for cellular function and have become a broad field of study. In cardio-renal diseases, it has been established that mitochondrial dysfunction is primary mechanism leading to these pathologies. Under stress, mitochondria can develop stress response mechanisms maintain quality control (MQC) functions. contrast, the perturbation associated with pathogenesis several diseases. Thus, targeting specific pathways within MQC could offer therapeutic avenue protecting integrity. However, related signaling in axis poorly explored. The limitations include lack reproducibility experimental models disease, incomplete knowledge molecules generate bidirectional damage, temporality study models. Therefore, we believe integration all those limitations, along recent advances (i.e., mitophagy), (e.g., integrated response, unfolded protein import), pharmacology, targeted approaches reveal what deregulation like provide ideas generating strategies seek avoid progression

Language: Английский

Citations

0
Transcriptomic insights into the resistance mechanism of Penaeus vannamei against highly lethal Vibrio parahaemolyticus DOI Creative Commons
Zhi‐Hao Huang, Yifei Liao, Jialiang Du

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 18, 2025

Abstract Highly lethal Vibrio disease (HLVD) caused by a virulent strain of parahaemolyticus ( Vp HLVD ), which poses significant threat to Penaeus vannamei post-larvae, leads substantial mortality and economic losses. To address this challenge, researchers have recently isolated highly disease-resistant P. shrimp. However, the underlying mechanisms that could improve resistance require further investigation. Our study found shrimp exhibited remarkable ability prevent invasion effectively. unravel genetic basis resistance, we conducted transcriptomic analysis with susceptible at various time points (0, 6, 12 h) post-infection . Differential gene expression (DEGs) uninfected revealed individuals displayed higher immune-related genes pathways compared their counterparts. Simultaneously, they lower toxin-binding colonization gene, indicating enhanced defense in resistant Upon infection, DEGs also showed attempt mount similar immune response as during early stages infection. infection progresses, strategies diverge between two groups, peak occurring later findings indicated did not experience stress are capable effectively enhancing middle late In summary, our understanding employed combat , would help develop effective for prevention control, ultimately reducing impact on aquaculture.

Language: Английский

Citations

0
Postbiotic Parabacteroides Distasonis Supplementation Enhances Intestinal and Skeletal Muscle Function in Aged Mice DOI Creative Commons

Pablo Morgado-Cáceres,

Hernán Huerta, Christiane Copie‐Bergman

et al.

Aging and Disease, Journal Year: 2025, Volume and Issue: unknown, P. 0 - 0

Published: Jan. 1, 2025

Language: Английский

Citations

0
EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases* DOI Creative Commons
Ipsita Nandi, Rachana Pattani Ramachandran, Deborah E. Shalev

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: Sept. 23, 2024

Enteropathogenic

Language: Английский

Citations

1
Stress Granules in Infectious Disease: Cellular Principles and Dynamic Roles in Immunity and Organelles DOI Open Access
J.-B. Kim, Chang‐Hwa Song

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12950 - 12950

Published: Dec. 2, 2024

Stress granules (SGs) are membrane-less aggregates that form in response to various cellular stimuli through a process called liquid-liquid phase separation (LLPS). Stimuli such as heat shock, osmotic stress, oxidative and infections can induce the formation of SGs, which play crucial roles regulating gene expression help cells adapt stress conditions. Various mRNAs proteins aggregated into particularly those associated with protein translation machinery, frequently found SGs. When induced by infections, SGs modulate immune cell activity, supporting against infection. The differ viral versus microbial depending on type involved, function differently In this review, we summarize our current understanding implication immunity organelles context infectious diseases. Importantly, explore insights regulatory functions host under

Language: Английский

Citations

1
EspH utilizes phosphoinositide and Rab binding domains to interact with plasma membrane infection sites and Rab GTPases DOI
Ipsita Nandi, Rachana Pattani Ramachandran, Deborah E. Shalev

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 29, 2024

Abstract Enteropathogenic E. coli (EPEC) is a Gram-negative bacterial pathogen that causes persistent diarrhea. Upon attachment to the apical plasma membrane of intestinal epithelium, translocates virulent proteins called effectors into infected cells. These hijack numerous host processes for pathogen’s benefit. Therefore, studying mechanisms underlying their action crucial better understanding disease. We show translocated EspH interacts with multiple Rab GTPases. AlphaFold predictions and site-directed mutagenesis identified glutamic acid lysine at positions 37 41 as interacting residues. Mutating these sites abolished ability inhibit Akt mTORC1 signaling, lysosomal exocytosis, invasion. Knocking out endogenous Rab8a gene expression highlighted involvement in Akt/mTORC1 signaling exocytosis. A phosphoinositide binding domain critical tyrosine was EspH. localization infection its capacity interact Rabs. Our data suggest novel EspH-dependent elicit immune trafficking during EPEC infection.

Language: Английский

Citations

0