Confocal Microscopy: Revolutionizing Three-Dimensional Imaging In Biological Research. DOI Open Access
Ashish Dwivedi

African Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: unknown, P. 7662 - 7671

Published: Dec. 16, 2024

Confocal microscopy is a high-resolution imaging technique that uses laser scanning to create sharp, three-dimensional images of biological samples, improving the visualization complex specimens.Confocal has wide range applications including stem cell and biofilm analysis, disease diagnosis etc. owing its ability capture detailed cellular structures, allowing for detection abnormalities in tissue morphology organization.The growing interest this technology underscores importance understanding foundations effectively address challenges further advance applications.This review explores history, principles, confocal biology, highlighting trends, challenges, future advancements technology.

Language: Английский

The Potential of Optical Technologies in Early Virus Detection; Prospects in Addressing Future Viral Outbreaks DOI
Mehdi Kheirollahpour, Nader Shokoufi, Mohsen Lotfi

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: March 27, 2025

The urgent need for sensitive, rapid, and reliable diagnostic methodologies to control prevent life-threatening pandemic infectious disease, such as COVID-19, remains a critical priority. Timely on-site detection of viral pathogens is essential effective disease management mitigation societal disruptions. Recent advancements in optical methods have positioned them at the forefront healthcare diagnostics, offering high sensitivity specificity viable alternatives conventional techniques Polymerase Chain Reaction (PCR), which often suffer from time delays limited accessibility resource-constrained environments. This review elucidates potential various techniques, highlighting their advantages over traditional methods. It encompasses range modalities, including fluorescence-based approaches, Raman spectroscopy (RS), Plasmonic (e.g., surface plasmon resonance (SPR), localized SPR, (LSPR), surface-enhanced (SERS), fluorescence (SEF)), super resolution microscopies (SRMs), attenuated total reflectance-Fourier transform infrared (ATR-FTIR), integrated platforms waveguides molecularly imprinted polymer (MIP)-based biosensors. Additionally, evolution novel biosensors, particularly 5th 6th generation challenges related these technologies were discussed. studies reviewed aims advance development portable, specific, cost-effective point-of-care (POC) devices rapid pathogens.

Language: Английский

Citations

0

Closing the multichannel gap through computational reconstruction of interaction in super-resolution microscopy DOI Creative Commons
Ben Cardoen, Hanene Ben Yedder, Ivan R. Nabi

et al.

Patterns, Journal Year: 2025, Volume and Issue: unknown, P. 101181 - 101181

Published: March 1, 2025

Language: Английский

Citations

0

The triple code model for advancing research in rare and undiagnosed diseases beyond the base pairs DOI Creative Commons
Gwen Lomberk, Raúl Urrutia

Epigenomics, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 10

Published: Dec. 4, 2024

Rare and undiagnosed diseases pose significant challenges for understanding their mechanisms, diagnosis, treatment. The Triple Code Model, an integrative paradigm described here, considers the combined influence of genetic code, epigenetic nuclear structure (an emerging code), as fundamental biochemical mechanisms underlying many rare diseases. Studies demonstrate dysfunctional membrane cytoplasmic signals instruct epigenome to ultimately impact 3D dynamics nucleus, highlighting close interrelationships. Consequently, this model offers a holistic perspective on by moving beyond solely view. We propose that integrated framework will efficiently guide disease research taking it 'Beyond Base Pairs,' leading improved diagnostics personalized treatments.

Language: Английский

Citations

0

Should Artificial Intelligence Play a Durable Role in Biomedical Research and Practice? DOI Open Access
Pierre Bongrand

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13371 - 13371

Published: Dec. 13, 2024

During the last decade, artificial intelligence (AI) was applied to nearly all domains of human activity, including scientific research. It is thus warranted ask whether AI thinking should be durably involved in biomedical This problem addressed by examining three complementary questions (i) What are major barriers currently met investigators? suggested that during 2 decades there a shift towards growing need elucidate complex systems, and this not sufficiently fulfilled previously successful methods such as theoretical modeling or computer simulation (ii) potential meet aforementioned need? it recent well-suited perform classification prediction tasks on multivariate possibly help data interpretation, provided their efficiency properly validated. (iii) Recent representative results obtained with machine learning suggest may comparable displayed operators. concluded play an important role practice. Also, already other physics, combining conventional might generate further progress new applications, involving heuristic interpretation.

Language: Английский

Citations

0

Confocal Microscopy: Revolutionizing Three-Dimensional Imaging In Biological Research. DOI Open Access
Ashish Dwivedi

African Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: unknown, P. 7662 - 7671

Published: Dec. 16, 2024

Confocal microscopy is a high-resolution imaging technique that uses laser scanning to create sharp, three-dimensional images of biological samples, improving the visualization complex specimens.Confocal has wide range applications including stem cell and biofilm analysis, disease diagnosis etc. owing its ability capture detailed cellular structures, allowing for detection abnormalities in tissue morphology organization.The growing interest this technology underscores importance understanding foundations effectively address challenges further advance applications.This review explores history, principles, confocal biology, highlighting trends, challenges, future advancements technology.

Language: Английский

Citations

0