How condensed are mitotic chromosomes? DOI
Hideaki Konishi, Hironori Funabiki

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(11)

Published: Oct. 14, 2024

Chromosomes undergo dramatic compaction during mitosis, but accurately measuring their volume has been challenging. Employing serial block face scanning electron microscopy, Cisneros-Soberanis et al. (https://doi.org/10.1083/jcb.202403165) report that mitotic chromosomes compact to a nucleosome concentration of ∼760 µM.

Language: Английский

Nanoscale analysis of human G1 and metaphase chromatin in situ DOI Creative Commons
Jon K. Chen, Tingsheng Liu, Shujun Cai

et al.

The EMBO Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

1
Dynamics of microcompartment formation at the mitosis-to-G1 transition DOI Creative Commons
Viraat Y. Goel, Nicholas Aboreden, James M. Jusuf

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

As cells exit mitosis and enter G1, mitotic chromosomes decompact transcription is reestablished. Previously, Hi-C studies showed that essentially all interphase 3D genome features including A/B-compartments, TADs, CTCF loops, are lost during mitosis. However, remains insensitive to such as microcompartments, nested focal interactions between cis -regulatory elements (CREs). We therefore applied Region Capture Micro-C from G1. Unexpectedly, we observe microcompartments in prometaphase, which further strengthen ana/telophase before gradually weakening Loss of loop extrusion through condensin depletion differentially impacts large suggesting they partially distinct. Using polymer modeling, show microcompartment formation favored by chromatin compaction disfavored activity, explaining why likely provides a particularly favorable environment. Our results suggest CREs exhibit intrinsic homotypic affinity leading formation, may explain transient transcriptional spiking observed upon exit.

Language: Английский

Citations

3
How condensed are mitotic chromosomes? DOI
Hideaki Konishi, Hironori Funabiki

The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(11)

Published: Oct. 14, 2024

Chromosomes undergo dramatic compaction during mitosis, but accurately measuring their volume has been challenging. Employing serial block face scanning electron microscopy, Cisneros-Soberanis et al. (https://doi.org/10.1083/jcb.202403165) report that mitotic chromosomes compact to a nucleosome concentration of ∼760 µM.

Language: Английский

Citations

0