Protein Purification Using Chitosan‐Modified Polyacrylonitrile Membranes DOI

Gizele G. F. Andrade,

Andressa Nathally Rocha Leal, Jeferson Gomes de Andrade

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

ABSTRACT Protein purification is essential for biomedical and industrial applications, but current methods often face challenges like low efficiency, high cost, limited selectivity. To overcome these drawbacks, we developed electrospun polyacrylonitrile (PAN) membranes with chitosan (Cs) evaluated their ability to capture bovine serum albumin (BSA) immunoglobulin G (IgG) from aqueous solutions. Comparing PAN/Cs pristine PAN membranes, showed Cs significantly enhances BSA adsorption. The were characterized using scanning electron microscopy, Fourier Transform Infrared Spectroscopy, tensile testing, surface area analysis, energy‐dispersive X‐ray spectroscopy, contact angle measurements. exhibited smooth, uniform fibers averaging 0.454 μm in diameter. maximum adsorption capacity () was 75.61 mg g −1 21.17 PAN, while IgG, 28.83 19.77 PAN. SDS‐PAGE electrophoresis confirmed that the eluted proteins maintained quality integrity. Elution efficiency of reached 89.13% 62.16% IgG. Additionally, selectively captured IgG when co‐dissolved These results position as promising materials efficient, cost‐effective, selective protein purification, potential applications both fields.

Language: Английский

Synthesis and characterization of Zn3Mn0.5Li0.2Ti4−xCexO12 nanostructures: spectroscopic and electrochemical insights for enhanced storage performance DOI
Amany M. El Nahrawy, M. K. Seddeek

Applied Physics A, Journal Year: 2025, Volume and Issue: 131(3)

Published: Feb. 5, 2025

Language: Английский

Citations

0
Impedance Spectroscopy and Antimicrobial Activity in ZnO–Cellulose/Chitosan@Cu2+ Wavy‐Like Nanocomposites Formed via Semi‐Dissolved Chitosan Templating DOI

Amel Mohamed Abouelnaga

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(3)

Published: March 1, 2025

ABSTRACT In this study, an advanced approach to synthesizing wavy ZnO–cellulose/chitosan@Cu 2+ nanocomposites with improved functionality and conductivity was semi‐dissolving chitosan as a dual‐functionality template. The template method uses form semi‐porous structures ethylene glycol the solvent crystallization agent for hybrid nanostructures. surface morphology, stability, conductive nano‐nanostructures significantly improve microstructural, electrical, antimicrobial properties of Cu‐doped ZnO–cellulose/chitosan formed composite morphology thermal are characterized by thermogravimetric analysis (TGA) field emission scanning electron microscope (SEM–EDX). revealed that nanostructures exhibited steady‐state plateau at low frequencies, which represents DC conductivity, while transferred frequency‐dependent increase higher frequencies due hopping conduction mechanism, Ac conductivity. impedance spectroscopy represented Cole–Cole indicated non‐Debye relaxation processes. equivalent circuit parameters estimated different samples showed segmental capacitance (C1) resistance (R1) values ranging from 4.5665 × 10 −11 F 2.3882 −10 4.9 6 Ω 5.244 5 Ω, Cu concentration increased zero (A0) 4 mol%(A2), respectively. activity using Well diffusion technique. demonstrated inhibition zone diameters 19 31 mm against various microorganisms, including 30 Aspergillus fumigatus 28 Escherichia coli A3 (6 mol% ). These findings suggest can be utilized in biomedical fields such wound dressings, environmental conservation, food packaging, coatings.

Language: Английский

Citations

0
Synthesis and Characterization of Coenzyme Q10 onto Nanoporous Calcium Silicate-Based Systems for Wound Healing DOI Creative Commons
H. Ismail,

Doaa Ahmed El-Setouhy,

Basant A. Habib

et al.

ECS Journal of Solid State Science and Technology, Journal Year: 2024, Volume and Issue: 13(8), P. 083010 - 083010

Published: Aug. 1, 2024

This study explores the microstructure, spectroscopic, and bonding arrangements within bioactive calcium silicate magnesium systems loaded with different values (1–2.5 wt%) Coenzyme (CoQ 10 ) ratios, synthesized using sol-gel processes. The investigation utilizes X-ray diffraction (XRD), transmission electron microscopy, Fourier transform infrared (FTIR) spectroscopy to analyze samples. assignment of FTIR bonds, examining changes in silicate-based bonds environment under effect CoQ10 ratio. observed frequency shifts intensity variations linked composition, are attributed a reduction local symmetry resulting from introducing oxides CoQ silica network. XRD results contribute valuable insights into structural role materials , thereby enhancing our understanding release process. Approval drug loading both nanosystems was recognized by bands, particle distribution, valuation activity showed two nanoparticles. Additionally, wound healing studies revealed biocompatibility response

Language: Английский

Citations

0
Protein Purification Using Chitosan‐Modified Polyacrylonitrile Membranes DOI

Gizele G. F. Andrade,

Andressa Nathally Rocha Leal, Jeferson Gomes de Andrade

et al.

Journal of Applied Polymer Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 20, 2024

ABSTRACT Protein purification is essential for biomedical and industrial applications, but current methods often face challenges like low efficiency, high cost, limited selectivity. To overcome these drawbacks, we developed electrospun polyacrylonitrile (PAN) membranes with chitosan (Cs) evaluated their ability to capture bovine serum albumin (BSA) immunoglobulin G (IgG) from aqueous solutions. Comparing PAN/Cs pristine PAN membranes, showed Cs significantly enhances BSA adsorption. The were characterized using scanning electron microscopy, Fourier Transform Infrared Spectroscopy, tensile testing, surface area analysis, energy‐dispersive X‐ray spectroscopy, contact angle measurements. exhibited smooth, uniform fibers averaging 0.454 μm in diameter. maximum adsorption capacity () was 75.61 mg g −1 21.17 PAN, while IgG, 28.83 19.77 PAN. SDS‐PAGE electrophoresis confirmed that the eluted proteins maintained quality integrity. Elution efficiency of reached 89.13% 62.16% IgG. Additionally, selectively captured IgG when co‐dissolved These results position as promising materials efficient, cost‐effective, selective protein purification, potential applications both fields.

Language: Английский

Citations

0