Improvement of gain and spatial resolution for impulsive stimulated Brillouin scattering microscopy

Photoacoustics, Journal Year: 2025, Volume and Issue: 42, P. 100696 - 100696

Published: Feb. 10, 2025

Brillouin microscopy has been widely used in the mechanical imaging of cells and tissues, signal-to-noise (SNR) ratio limits spectral integration time. Impulsive stimulated scattering (ISBS) is a new elastic technique. As variant (SBS), ISBS can overcome weak signal spontaneous scattering. A simple model estimate SBS gain. However, theoretical gain not compared with This paper gives estimation, experiments are designed to verify estimation reliability. The heterodyne coefficient be much higher than coefficient. relationship between spatial resolution then discussed. We anticipate that setup optimization improve gain, potentially enabling fast high biological cells.

Language: Английский

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Chlorella-enriched hydrogels protect against myocardial damage and reactive oxygen species production in an in vitro ischemia/reperfusion model using cardiac spheroids

Biofabrication, Journal Year: 2024, Volume and Issue: 17(1), P. 015006 - 015006

Published: Oct. 2, 2024

Abstract Microalgae have emerged as promising photosynthetic microorganisms for biofabricating advanced tissue constructs, with improved oxygenation and reduced reactive oxygen species (ROS) production. However, their use in the engineering of human tissues has been limited due to intrinsic growth requirements, which are not compatible cells. In this study, we first formulated alginate–gelatin (AlgGel) hydrogels increasing densities Chlorella vulgaris . Then, characterised mechanical properties pore size. Finally, evaluated effects on cardiac spheroid (CS) pathophysiological response under control ischemia/reperfusion (I/R) conditions. Our results showed that addition did affect AlgGel properties, while mean size significantly decreased by 35% presence 10 7 cells ml −1 microalgae density. Under normoxic conditions, CS viability starting from 14 d in. No changes nor were measured containing 5 6 our I/R model, all -enriched cell sensitivity hypoxic conditions a corresponding reduction ROS production, well protected against I/R-induced viability. Altogether, support Alg–Gel cardiovascular engineering.

Language: Английский

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Brown Adipose Stem Cell-Loaded Resilin Elastic Hydrogel Rebuilds Cardiac Function after Myocardial Infarction via Collagen I/III Reorganisation

Gels, Journal Year: 2024, Volume and Issue: 10(9), P. 568 - 568

Published: Aug. 31, 2024

Irreversible fibrosis following myocardial infarction (MI) stiffens the infarcted myocardium, which remains challenging to restore. This study aimed investigate whether injectable RLP12 hydrogel, derived from recombinant resilin protein, could serve as a vehicle for stem cells enhance function of myocardium. The hydrogel was prepared and injected into myocardium rats with MI, brown adipose-derived mesenchymal (BADSCs) were loaded. survival differentiation BADSCs in vivo investigated using immunofluorescence one week four weeks after treatment, respectively. heart function, MI area, collagen deposition, microvessel density further assessed treatment through echocardiography, histology, immunohistochemistry, immunofluorescence. shear modulus 10–15 kPa. Four transplantation, significantly improved cardiac by increasing reducing infarct area size deposition rats. Furthermore, distribution ratio III I increased both centre edge areas indicating compliance heart. Moreover, also promoted troponin T- α-smooth muscle-positive cells. can be utilised an treating regulating expression profiles improve mechanical microenvironment site, thereby restoring function. provides novel insights interactions between microenvironment.

Language: Английский

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3D in vitro Modelling of Post-Partum Cardiovascular Health reveals Unique Characteristics and Signatures following Hypertensive Disorders in Pregnancy

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 17, 2024

Background Hypertensive disorders of pregnancy (HDP) affect 2–8% pregnancies and are associated postpartum with increased cardiovascular disease (CVD) risk, although mechanisms poorly understood. Methods Human iPSC-derived cardiomyocytes, cardiac fibroblasts coronary artery endothelial cells were tri-cultured to form spheroids (CS) in collagen type-1 hydrogels containing 10% patient plasma collected five years (n = 5 per group: normotensive control, gestational hypertension (GH) preeclampsia (PE). Plasma-treated CS assessed for cell viability contractile function subjected immunofluorescence staining imaging. A quantitative proteomic analysis samples was conducted (controls n 21; GH 5; PE 12). Results Contraction frequency (CF) PE-treated (CF:45.5 ± 3.4 contractions/minute, p < 0.001) GH-treated (CF:45.7 4.0 0.001), compared controls (CF 21.8 2.6 contractions/minute). Only presented significantly fractional shortening (FS) % (9.95 1.8%, 0.05) (3.7 1.1%). CSs showed a reduction (p an increase α-SMA expression 0.05). Proteomics identified twenty differentially abundant proteins, hemoglobin A2 being the only protein perturbed both versus control Conclusions Using innovative patient-relevant platforms led discovery biomarkers/targets linked death signaling remodeling GH-induced CVD vascular/endothelial dysfunction PE-induced CVD.

Language: Английский

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3D in vitro modelling of post-partum cardiovascular health reveals unique characteristics and signatures following hypertensive disorders in pregnancy

Biology of Sex Differences, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 25, 2024

Hypertensive disorders of pregnancy (HDP) affect 2-8% pregnancies and are associated postpartum with increased cardiovascular disease (CVD) risk, although mechanisms poorly understood.

Language: Английский

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Advances in 3D Bioprinting Technologies for Cardiovascular Intervention and Regeneration Therapeutics

Journal of Cardiovascular Intervention, Journal Year: 2024, Volume and Issue: 4

Published: Dec. 5, 2024

Language: Английский

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