Toward Resolving Heterogeneous Mixtures of Nanocarriers in Drug Delivery Systems through Light Scattering and Machine Learning DOI

Allan Mancoo,

Mariana Silva, C. Lopes

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 8, 2025

Nanocarriers (NCs) have emerged as a revolutionary approach in targeted drug delivery, promising to enhance efficacy and reduce toxicity through precise targeting controlled release mechanisms. Despite their potential, the clinical adoption of NCs is hindered by challenges physicochemical characterization, essential for ensuring safety, efficacy, quality control. Traditional characterization methods, such dynamic light scattering nanoparticle tracking analysis, offer limited insights, primarily focusing on particle size concentration, while techniques like high-performance liquid chromatography mass spectrometry are hampered extensive sample preparation, high costs, potential degradation. Addressing these limitations, this work presents cost-effective methodology leveraging optical forces, combined with machine learning algorithms, characterize polydisperse mixtures, including lipid-based NCs. We prove that our provides quantification relative concentration complex suspensions detecting changes refractive index polydispersity without preparation or destruction, offering high-throughput solution NC delivery systems. Experimental validation demonstrates method's characterizing commercially available synthetic nanoparticles Doxoves, liposomal formulation Doxorubicin used cancer treatment, marking significant advancement toward reliable, noninvasive can accelerate translation nanocarrier-based therapeutics.

Language: Английский

Investigation of Soft Matter Nanomechanics by Atomic Force Microscopy and Optical Tweezers: A Comprehensive Review DOI Creative Commons
Alessandro Magazzù, Carlos Marcuello

Nanomaterials, Journal Year: 2023, Volume and Issue: 13(6), P. 963 - 963

Published: March 7, 2023

Soft matter exhibits a multitude of intrinsic physico-chemical attributes. Their mechanical properties are crucial characteristics to define their performance. In this context, the rigidity these systems under exerted load forces is covered by field biomechanics. Moreover, cellular transduction processes which involved in health and disease conditions significantly affected exogenous biomechanical actions. framework, atomic force microscopy (AFM) optical tweezers (OT) can play an important role determine parameters investigated at single-molecule level. This review aims fully comprehend interplay between soft systems. particular, we outline capabilities AFM OT compared other classical bulk techniques nanomechanical such as Young’s modulus. We also provide some recent examples measurements performed using hydrogels, biopolymers systems, among others. expect present manuscript will aid potential readers stakeholders understand applications

Language: Английский

Citations

114
Transverse optical gradient force in untethered rotating metaspinners DOI Creative Commons
Einstom Engay, Mohammad Mahdi Shanei, Vasilii Mylnikov

et al.

Light Science & Applications, Journal Year: 2025, Volume and Issue: 14(1)

Published: Jan. 8, 2025

Language: Английский

Citations

3
Metareview: a survey of active matter reviews DOI Creative Commons
Michael te Vrugt, Raphael Wittkowski

The European Physical Journal E, Journal Year: 2025, Volume and Issue: 48(2)

Published: Feb. 1, 2025

In the past years, amount of research on active matter has grown extremely rapidly, a fact that is reflected in particular by existence more than 1000 reviews this topic. Moreover, field become very diverse, ranging from theoretical studies statistical mechanics particles to applied work medical applications microrobots and biological systems artificial swimmers. This makes it difficult get an overview over as whole. Here, we provide such form metareview article surveys existing review articles books matter. Thereby, provides useful starting point for finding literature about specific

Language: Английский

Citations

2
Generating free-space structured light with programmable integrated photonics DOI Creative Commons
Johannes Bütow, Jörg S. Eismann, V. Sharma

et al.

Nature Photonics, Journal Year: 2024, Volume and Issue: 18(3), P. 243 - 249

Published: Jan. 12, 2024

Language: Английский

Citations

14
Optothermal Revolution: Colloids in an Optical Ring Trap DOI

Rahul Chand,

Ashutosh Shukla, G. V. Pavan Kumar

et al.

ACS Photonics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Directional motion is commonly observed in various living active systems, such as bacterial colonies moving through confined environments. In these the dynamics arise from collective effects of mutual interactions between individual elements, well their with obstacles or boundaries. this study, we turn our focus to an artificial system and experimentally investigate emergence directional revolution dimer trimer structures composed colloidal particles ring-shaped optical illumination. case, movement exclusively facilitated by optothermal interactions─without any direct mechanical force applied external field. Depending on absorption properties particles, can exhibit both attractive repulsive characteristics. The provide necessary driving that propels motion, while serve control structural parameters system. arrangement interaction within fuel controlled, revolution, gradient acting a confining factor, guiding along specific path. Notably, systems be tuned altering intensity This study useful model for understanding insights into biological where group environmental are key coordinated movement.

Language: Английский

Citations

1
Light, Matter, Action: Shining Light on Active Matter DOI Creative Commons
Marcel Rey, Giovanni Volpe, Giorgio Volpe

et al.

ACS Photonics, Journal Year: 2023, Volume and Issue: 10(5), P. 1188 - 1201

Published: April 17, 2023

Light carries energy and momentum. It can therefore alter the motion of objects on atomic to astronomical scales. Being widely available, readily controllable, broadly biocompatible, light is also an ideal tool propel microscopic particles, drive them out thermodynamic equilibrium, make active. Thus, light-driven particles have become a recent focus research in field soft active matter. In this Perspective, we discuss advances control matter with light, which has mainly been achieved using intensity. We highlight some first attempts utilize light's additional properties, such as its wavelength, polarization, then argue that fully exploiting all properties will play critical role increasing level over actuation well flow itself through it. This enabling step advance design systems, their functionalities, transfer toward technological applications.

Language: Английский

Citations

18
Optical Forces on Multipoles Induced by the Belinfante Spin Momentum DOI
Yuan Zhou, Yanan Zhang, Xiaohao Xu

et al.

Laser & Photonics Review, Journal Year: 2023, Volume and Issue: 17(11)

Published: June 27, 2023

Abstract The Belinfante spin momentum (BSM) is a fundamental yet enigmatic quantity of electromagnetic fields. It vanishes from the global field, but can be detected locally, manifesting itself as an optical force on small particles. Hitherto, however, BSM concept well established only within dipole approximation, and there no explicit experimental evidence for its action multipoles. Here, theoretical model multipolar forces, exerted generic Mie particles supporting multipoles arbitrary order, developed. This mechanical observed experimentally structured light which suppresses effect spin–orbit coupling. also selectively enhance while restraining dipolar component probe particle (Au sphere). These results constitute distinct chapter in physics high‐order interactions light–matter systems facilitate additional progress optomechanics, manipulation, Mie‐tronics.

Language: Английский

Citations

18
Topology of molecular deformations induces triphasic catch bonding in selectin–ligand bonds DOI Creative Commons
Casey O. Barkan, Robijn Bruinsma

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(6)

Published: Jan. 31, 2024

Among the long-standing efforts to elucidate physical mechanisms of protein–ligand catch bonding, particular attention has been directed at family selectin proteins. Selectins exhibit slip, catch–slip, and slip–catch–slip with minor structural modifications causing major changes in selectins’ response force. How can a single mechanism allow interconversion between these various behaviors? We present unifying theory selectin–ligand using structurally motivated free energy landscape show how topology force-induced deformations molecular system produces full range observed behaviors. find that pathway bond rupture deforms non-trivial ways, such unbinding dynamics depend sensitively on This implies severe breakdown Bell’s theory—a paradigmatic used widely modeling—raising questions about suitability modeling other bonds. Our approach be applied broadly systems.

Language: Английский

Citations

8
Integrated Microfluidics for Single‐Cell Separation and On‐Chip Analysis: Novel Applications and Recent Advances DOI Creative Commons
Hazal Kutluk, Martina Viefhues, Iordania Constantinou

et al.

Small Science, Journal Year: 2024, Volume and Issue: 4(4)

Published: Feb. 2, 2024

From deciphering infection and disease mechanisms to identifying novel biomarkers personalizing treatments, the characteristics of individual cells can provide significant insights into a variety biological processes facilitate decision‐making in biomedical environments. Conventional single‐cell analysis methods are limited terms cost, contamination risks, sample volumes, times, throughput, sensitivity, selectivity. Although microfluidic approaches have been suggested as low‐cost, information‐rich, high‐throughput alternative conventional isolation methods, limitations such necessary off‐chip pre‐ post‐processing well systems designed for workflows restricted their applications. In this review, comprehensive overview recent advances integrated microfluidics on‐chip three prominent application domains provided: investigation somatic (particularly cancer immune cells), stem cells, microorganisms. Also, use cell separation (e.g., dielectrophoresis) unconventional or ways, which advance integration multiple systems, is discussed. Finally, critical discussion related current how they could be overcome provided.

Language: Английский

Citations

8
Programmable photoacoustic patterning of microparticles in air DOI Creative Commons

Ruoqin Zhang,

Xichuan Zhao,

Jinzhi Li

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 16, 2024

Abstract Optical and acoustic tweezers, despite operating on different physical principles, offer non-contact manipulation of microscopic mesoscopic objects, making them essential in fields like cell biology, medicine, nanotechnology. The advantages limitations optical complement each other, particularly terms trapping size, force intensity, flexibility. We use photoacoustic effects to generate localized Lamb wave capable mapping arbitrary laser pattern shapes. By using waves vibrate the surface multilayer membrane, we can tens thousands particles into desired simultaneously. Moreover, by quickly successively adjusting shape, microparticles flow dynamically along corresponding elastic fields, creating a frame-by-frame animation. Our approach merges programmable adaptability tweezers with potent capabilities waves, paving way for wave-based techniques, such as microparticle assembly, biological synthesis, microsystems.

Language: Английский

Citations

7