Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 423, P. 136729 - 136729
Published: Oct. 2, 2024
Language: Английский
North American Journal of Aquaculture, Journal Year: 2025, Volume and Issue: unknown
Published: March 18, 2025
ABSTRACT Objective The larvae death caused by the infection of Micropterus salmoides rhabdovirus (MSRV) has seriously affected development Largemouth Bass nigricans industry. Therefore, it is necessary to establish a fast, convenient, and sensitive detection method detect MSRV-positive fish quickly control virus transmission. Methods In this study, primers probes were designed based on G gene MSRV. Through primer screening, specificity, sensitivity, repeatability tests, reverse transcription multienzyme isothermal rapid amplification (RT-MIRA) combined with lateral flow dipstick (LFD) was established. Additionally, we applied published transcription-polymerase chain reaction simultaneously clinical samples. Results temperature 42°C, total duration entire process 35 min. specificity test revealed that MSRV could be specifically detected had no cross-reaction other common susceptible pathogens fish. sensitivity indicated limit for recombinant plasmid standard 102 copies/μL. repetitive showed good reproducibility. There significant difference between results RT-MIRA-LFD RT-PCR. Conclusions established in study detecting simple, highly sensitive, specific, without need precise or expensive equipment. suitable resource-limited laboratories on-site detection.
Language: Английский
Citations
0
Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 113779 - 113779
Published: April 1, 2025
Language: Английский
Citations
0
Animals, Journal Year: 2024, Volume and Issue: 14(5), P. 682 - 682
Published: Feb. 22, 2024
The Caliciviridae family includes several viral pathogens of humans and animals, including norovirus (NoV), genus Norovirus, feline calicivirus (FCV), Vesivirus. Due to their resistance in the environment, NoV FCV may give rise nosocomial infections, indirect transmission plays a major role diffusion susceptible populations. A pillar control viruses resistant an environment is adoption prophylaR1.6ctic measures, disinfection. Since NoVs are not cultivatable common cell cultures, has been largely used as surrogate for assessment effective disinfectants. Ozone (O3), molecule with strong oxidizing properties, shown microbicidal activity on bacteria, fungi, protozoa, viruses. In this study, virucidal antiviral activities O3/O2 gas mixture containing O3 were tested at different concentrations (20, 35, 50 μg/mL) distinct contact times against FCV. showed dose- time-dependent fashion. Ozonation could be considered valid strategy disinfection environments risk contamination by NoV.
Language: Английский
Citations
0
Foodborne Pathogens and Disease, Journal Year: 2024, Volume and Issue: 21(12), P. 738 - 744
Published: Aug. 26, 2024
Highlighting rotavirus (RV) as a significant food and waterborne pathogen, particularly affecting infants children, causing serious gastrointestinal infections dehydration, is important. It should be noted that there are debates regarding the effectiveness outcomes of RV vaccination. In contrast to Turkey's nonmandatory vaccination policy, many developed countries implement mandatory policies, raising questions about their impact on disease prevalence healthcare expenditures. Our study aims comprehensively understand in Turkey compare them with have policies. We found similar, even better, hospitalization rates, length hospital stays, laboratory parameters demonstrating independent approach. These findings contribute valuable insights global strategies control.
Language: Английский
Citations
0
Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 423, P. 136729 - 136729
Published: Oct. 2, 2024
Language: Английский
Citations
0