High-speed
multiplex
Stimulated
Raman
Scattering
(SRS)
microscopy
has
emerged
as
a
powerful
imaging
technique
in
the
field
of
biomedical
research.
This
cutting-edge
technology
combines
benefits
traditional
spectroscopy
with
high
speed
and
resolution
microscopy,
enabling
real-time,
label-free,
non-invasive
visualization
biological
samples
at
molecular
level.
In
this
article,
we
delve
into
principles,
advantages,
applications
high-speed
SRS
context
studies.
Biomedical Optics Express,
Journal Year:
2023,
Volume and Issue:
14(11), P. 5764 - 5764
Published: Sept. 28, 2023
Triple
negative
breast
cancer
(TNBC)
is
a
highly
aggressive
form
of
cancer.
Detecting
TNBC
early
crucial
for
improving
disease
prognosis
and
optimizing
treatment.
Unfortunately,
conventional
imaging
techniques
fall
short
in
providing
comprehensive
differentiation
subtypes
due
to
their
limited
sensitivity
inability
capture
subcellular
details.
In
this
study,
we
present
multimodal
platform
that
integrates
heavy
water
(D
2
O)-probed
stimulated
Raman
scattering
(DO-SRS),
two-photon
fluorescence
(TPF),
second
harmonic
generation
(SHG)
imaging.
This
allows
us
directly
visualize
quantify
the
metabolic
activities
at
level.
By
utilizing
DO-SRS
imaging,
were
able
identify
distinct
levels
de
novo
lipogenesis,
protein
synthesis,
cytochrome
c
heterogeneity,
lipid
unsaturation
rates
various
subtype
tissues.
Simultaneously,
TPF
provided
spatial
distribution
mapping
NAD[P]H
flavin
signals
tissues,
revealing
high
redox
ratio
significant
turnover
rate
BL2
(HCC1806)
samples.
Furthermore,
SHG
enabled
observe
diverse
orientations
collagen
fibers
with
higher
anisotropy
tissue
boundary
compared
center.
Our
offers
sensitive
approach
characterizing
not
only
TNBC,
but
also
other
cancers.
The Analyst,
Journal Year:
2024,
Volume and Issue:
149(19), P. 4789 - 4810
Published: Jan. 1, 2024
One
of
the
challenges
facing
biology
is
to
understand
metabolic
events
at
a
single
cellular
level.
While
approaches
examine
dynamics
protein
distribution
or
report
on
spatiotemporal
location
signalling
molecules
are
well-established,
tools
for
dissection
metabolism
in
living
cells
less
common.
Advances
Raman
spectroscopy,
such
as
stimulated
scattering
(SRS),
beginning
offer
new
insights
into
range
experimental
systems,
including
model
organisms
and
clinical
samples,
across
disciplines.
Despite
power
imaging,
it
remains
relatively
under-used
technique
approach
biological
problems,
part
because
specialised
nature
analysis.
To
raise
profile
this
method,
here
we
consider
some
key
studies
which
illustrate
how
spectroscopy
has
revealed
fatty
acid
lipid
systems.
The
powerful
non-invasive
offers
suite
biomolecular
scientists
address
within
informs
underpins
function.
We
potential
applications,
discuss
recent
advances,
direction
travel
research
area.
Cancer Discovery,
Journal Year:
2024,
Volume and Issue:
14(11), P. 2071 - 2088
Published: Nov. 1, 2024
Abstract
Precision
oncology
tailors
treatment
strategies
to
a
patient’s
molecular
and
health
data.
Despite
the
essential
clinical
value
of
current
diagnostic
methods,
hematoxylin
eosin
morphology,
immunohistochemistry,
gene
panel
sequencing
offer
an
incomplete
characterization.
In
contrast,
highly
multiplexed
tissue
imaging
allows
spatial
analysis
dozens
markers
at
single-cell
resolution
enabling
complex
tumor
ecosystems;
thereby
it
has
potential
advance
our
understanding
cancer
biology
supports
drug
development,
biomarker
discovery,
patient
stratification.
We
describe
available
modalities,
discuss
their
advantages
disadvantages
for
use,
paths
implement
these
into
practice.
Significance:
This
review
provides
guidance
on
how
high-resolution,
samples
can
be
integrated
workflows.
It
systematically
compares
existing
emerging
technologies
outlines
applications
in
field
precision
oncology,
bridging
ever-evolving
landscape
research
with
practical
implementation
possibilities
routine
We
have
developed
a
novel
methodology
to
capture
images
of
various
biomolecules
at
resolution
surpassing
the
traditional
diffraction
limit
optical
microscopy.
By
harnessing
multimodal
imaging
platform
that
combines
stimulated
Raman
scattering
(SRS),
multiphoton
fluorescence
(MPF),
and
second
harmonic
generation
(SHG),
together
with
sophisticated
image
deconvolution
algorithms,
we
successfully
generated
super-resolution
reveal
details
biomolecular
metabolism.
These
enable
us
explore
intricate
associations
between
metabolic
activities
spatial
distribution
metabolites
within
breast
cancer
tissues.
To
enhance
accuracy
this
measurement
technique,
in
study,
designed
pre-processing
workflow
incorporates
both
denoising
drift
correction
processes.
Our
cutting-edge,
nonlinear
approach,
when
applied
context
new
workflow,
holds
significant
promise
for
advancing
early
detection
cancer,
prognostication,
evaluation
therapeutic
outcomes,
deepening
our
mechanistic
understanding
diseases.
We
have
developed
a
novel
stimulated
Raman
scattering
(SRS)
imaging
platform
to
visualize
various
biomolecules
at
resolution
beyond
the
traditional
diffraction
limit
of
optical
microscopy.
Applying
super-resolution
deconvolution
algorithm,
Adam
based
Pointillism
Deconvolution
(A-PoD),
hyperspectral
SRS
images,
we
can
measure
nanoscopic
distributions
these
molecules
in
cells.
In
this
study,
showcase
application
A-PoD
for
images
lipids,
protein,
unsaturated
lipid,
and
saturated
lipids
rat
olfactory
bulbs
under
hypoxia.
The
analysis
result
shows
distinct
lipid
glomeruli
hypoxia
control
rats.
This
approach
capability
revealing
molecular
composition
metabolic
activities.
When
applied
context
with
new
workflow,
holds
significant
promise
advancing
early
detection
understanding
diseases.
