High speed multiplex SRS microscopy for biomedical studies DOI
Zhi Li, Yajuan Li, Jorge Villazon

et al.

Published: Oct. 5, 2023

High-speed multiplex Stimulated Raman Scattering (SRS) microscopy has emerged as a powerful imaging technique in the field of biomedical research. This cutting-edge technology combines benefits traditional spectroscopy with high speed and resolution microscopy, enabling real-time, label-free, non-invasive visualization biological samples at molecular level. In this article, we delve into principles, advantages, applications high-speed SRS context studies.

Language: Английский

The applications of bio-orthogonal Raman labels for visualizing lipids in eukaryotic cells DOI

Ning Xu,

Dawei Sun, Hongwei Sun

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 183, P. 118099 - 118099

Published: Dec. 12, 2024

Language: Английский

Citations

0
Metabolic nanoscopy enhanced by experimental and computational approaches DOI Creative Commons

Hongje Jang,

Shuang Wu, Yajuan Li

et al.

npj Imaging, Journal Year: 2024, Volume and Issue: 2(1)

Published: Dec. 12, 2024

The advances in microscopy techniques have led to new findings biology. recent super-resolution technologies revealed precise molecular distribution. visualize the distributions of multiple molecules biological samples from experimental computational approaches are reviewed. By summarizing techniques, future direction collaborative research is highlighted show nanoscopic chemical details samples.

Language: Английский

Citations

0
Seeing the Action of Lipid Droplets in Aging and Longevity DOI
Bo Tao, Hongyuan Yang, Rong Fan

et al.

GEN Biotechnology, Journal Year: 2023, Volume and Issue: 2(4), P. 287 - 289

Published: Aug. 1, 2023

Language: Английский

Citations

0
Super resolution multimodal microscopy for studying spatially correlated metabolism in aging and diseases DOI Open Access
Hongje Jang, Zhi Li, Ellen Ackerstaff

et al.

Published: Oct. 5, 2023

In this study, we developed a new technique to analyze spatial correlation between metabolic activities of multiple biomolecules. Using multimodal imaging platform integrating Stimulated Raman Scattering (SRS), Multiphoton Fluorescence (MPF), and Second Harmonic Generation (SHG), together with an image deconvolution algorithm, obtained super resolution images biomolecular metabolism investigated the correlations distributions metabolites in tissues such as breast cancer tissues. Further, Pearson's Correlation Coefficient based algorithm examine co-registration co-regulation channels super-resolved nanoscopic Regions Interest (ROIs). The coefficient-based potentially facilitate early-stage detection, mechanistic understanding cancer.

Language: Английский

Citations

0
High speed multiplex SRS microscopy for biomedical studies DOI
Zhi Li, Yajuan Li, Jorge Villazon

et al.

Published: Oct. 5, 2023

High-speed multiplex Stimulated Raman Scattering (SRS) microscopy has emerged as a powerful imaging technique in the field of biomedical research. This cutting-edge technology combines benefits traditional spectroscopy with high speed and resolution microscopy, enabling real-time, label-free, non-invasive visualization biological samples at molecular level. In this article, we delve into principles, advantages, applications high-speed SRS context studies.

Language: Английский

Citations

0