Pulsed Blue Light and Phage Therapy: A Novel Synergistic Bactericide DOI Creative Commons
Amit Rimon, Jonathan Belin, Ortal Yerushalmy

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 481 - 481

Published: May 9, 2025

Background: Antibiotic-resistant Pseudomonas aeruginosa (P. aeruginosa) strains are an increasing cause of morbidity and mortality. Pulsed blue light (PBL) enhances porphyrin-induced reactive oxygen species has been clinically shown to be harmless the skin at low doses. Bacteriophages, viruses that infect bacteria, offer a promising non-antibiotic bactericidal approach. This study investigates potential synergism between low-dose PBL phage therapy against P. in planktonic cultures preformed biofilms. Methods: We conducted factorial dose–response vitro combining aeruginosa-specific phages with (457 nm, 33 kHz) on both PA14 multidrug-resistant PATZ2 strains. After excluding direct effects titer or activity, we assessed effectiveness using growth curve analysis, CFU, PFU. Biofilm efficacy was evaluated CFU post-sonication, crystal violet staining, live/dead staining confocal microscopy. Finally, (ROS) as mechanism nitro tetrazolium reduction assay. ANOVA Kruskal–Wallis tests post hoc Tukey Conover–Iman were used for comparisons (n = 5 biological replicates technical triplicates). Results: The bacterial lag phase significantly extended alone alone, synergistic effect up 144% (p < 0.001 all), achieving 9 log CFU/mL 24 h 0.001). In biofilms, combinations reduced biofilm biomass viability (% Live, median (IQR): Control 80%; Phage 40%; 25%; PBL&Phage 15%, p Mechanistically, triggered transient ROS cultures, amplified by co-treatment, while biphasic pattern biofilms reflected time-dependent synergy. Conclusions: combined demonstrates Given strong safety profile phages, this approach may lead novel, antibiotic-complementary, safe treatment modality patients suffering from difficult-to-treat antibiotic-resistant infections biofilm-associated infections.

Language: Английский

Pulsed Blue Light and Phage Therapy: A Novel Synergistic Bactericide DOI Creative Commons
Amit Rimon, Jonathan Belin, Ortal Yerushalmy

et al.

Antibiotics, Journal Year: 2025, Volume and Issue: 14(5), P. 481 - 481

Published: May 9, 2025

Background: Antibiotic-resistant Pseudomonas aeruginosa (P. aeruginosa) strains are an increasing cause of morbidity and mortality. Pulsed blue light (PBL) enhances porphyrin-induced reactive oxygen species has been clinically shown to be harmless the skin at low doses. Bacteriophages, viruses that infect bacteria, offer a promising non-antibiotic bactericidal approach. This study investigates potential synergism between low-dose PBL phage therapy against P. in planktonic cultures preformed biofilms. Methods: We conducted factorial dose–response vitro combining aeruginosa-specific phages with (457 nm, 33 kHz) on both PA14 multidrug-resistant PATZ2 strains. After excluding direct effects titer or activity, we assessed effectiveness using growth curve analysis, CFU, PFU. Biofilm efficacy was evaluated CFU post-sonication, crystal violet staining, live/dead staining confocal microscopy. Finally, (ROS) as mechanism nitro tetrazolium reduction assay. ANOVA Kruskal–Wallis tests post hoc Tukey Conover–Iman were used for comparisons (n = 5 biological replicates technical triplicates). Results: The bacterial lag phase significantly extended alone alone, synergistic effect up 144% (p < 0.001 all), achieving 9 log CFU/mL 24 h 0.001). In biofilms, combinations reduced biofilm biomass viability (% Live, median (IQR): Control 80%; Phage 40%; 25%; PBL&Phage 15%, p Mechanistically, triggered transient ROS cultures, amplified by co-treatment, while biphasic pattern biofilms reflected time-dependent synergy. Conclusions: combined demonstrates Given strong safety profile phages, this approach may lead novel, antibiotic-complementary, safe treatment modality patients suffering from difficult-to-treat antibiotic-resistant infections biofilm-associated infections.

Language: Английский

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