Klotho attenuates glucocorticoid-induced osteoblast cytotoxicity via Wnt signaling pathway modulation DOI
Sen Wang, Miao He, Xiao Liang

et al.

Published: April 28, 2025

Abstract Background Glucocorticoids are commonly prescribed in clinical settings; however, their prolonged use at high doses can adversely affect human health. One significant complication following glucocorticoid therapy is glucocorticoid-induced osteoporosis (GIO), which second incidence only to senile osteoporosis. Objective Based on previous research indicating that Klotho alleviates dexamethasone-induced osteoblast cytotoxicity through the NF-kB pathway, we aimed explore underlying mechanisms greater depth. Methods We assessed impact of Lithium chloride (LiCl), a Wnt pathway activator, cell and viability. Cytotoxicity was specifically quantified by Annexin V/PI flow cytometry. performed qRT-PCR Western blotting analyses scrutinize expressions genes proteins associated with both canonical non-canonical signaling pathways. Results Dexamethasone treatment induced an upregulation ligand, Wnt5a, downregulation Wnt3a, along its downstream marker, β-catenin. Transfection counteracted these effects. Conclusion has potential modulate pathways, thereby counteracting glucocorticoids.

Language: Английский

Klotho attenuates glucocorticoid-induced osteoblast cytotoxicity via Wnt signaling pathway modulation DOI
Sen Wang, Miao He, Xiao Liang

et al.

Published: April 28, 2025

Abstract Background Glucocorticoids are commonly prescribed in clinical settings; however, their prolonged use at high doses can adversely affect human health. One significant complication following glucocorticoid therapy is glucocorticoid-induced osteoporosis (GIO), which second incidence only to senile osteoporosis. Objective Based on previous research indicating that Klotho alleviates dexamethasone-induced osteoblast cytotoxicity through the NF-kB pathway, we aimed explore underlying mechanisms greater depth. Methods We assessed impact of Lithium chloride (LiCl), a Wnt pathway activator, cell and viability. Cytotoxicity was specifically quantified by Annexin V/PI flow cytometry. performed qRT-PCR Western blotting analyses scrutinize expressions genes proteins associated with both canonical non-canonical signaling pathways. Results Dexamethasone treatment induced an upregulation ligand, Wnt5a, downregulation Wnt3a, along its downstream marker, β-catenin. Transfection counteracted these effects. Conclusion has potential modulate pathways, thereby counteracting glucocorticoids.

Language: Английский

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