Biology,
Journal Year:
2023,
Volume and Issue:
12(2), P. 323 - 323
Published: Feb. 16, 2023
Toll-like
receptors
(TLRs)
have
been
implicated
in
the
regulation
of
various
metabolism
pathways,
addition
to
their
function
innate
immunity.
Here,
we
investigate
metabolic
TLR2
a
larval
zebrafish
system.
We
studied
larvae
from
tlr2
mutant
and
wild
type
sibling
controls
an
unchallenged
normal
developmental
condition
using
transcriptomic
metabolomic
analyses
methods.
RNAseq
was
used
evaluate
differences
between
wild-type
control
found
signature
set
149
genes
be
significantly
altered
gene
expression.
The
expression
level
several
confirmed
by
qPCR
analyses.
Gene
enrichment
analysis
(GSEA)
revealed
differential
two
genotypes
related
valine,
leucine,
isoleucine
degradation
glycolysis
gluconeogenesis.
Using
1H
nuclear
magnetic
resonance
(NMR)
metabolomics,
that
glucose
metabolites
with
were
present
at
higher
levels
mutant.
Furthermore,
is
mutants
fluorometric
assay.
Therefore,
shown
TLR2,
its
immunity,
has
controlling
during
vertebrate
development.
functions
are
associated
transcriptional
enzymes
involved
could
explain
different
glucose,
lactate,
succinate,
malate
Heliyon,
Journal Year:
2024,
Volume and Issue:
10(7), P. e28429 - e28429
Published: March 22, 2024
Rapid
ascent
to
high-altitude
areas
above
2500
m
often
leads
acute
high
altitude
illness
(AHAI),
posing
significant
health
risks.
Current
models
for
AHAI
research
are
limited
in
their
ability
accurately
simulate
the
environment
drug
screening.
Addressing
this
gap,
a
novel
static
self-assembled
water
vacuum
transparent
chamber
was
developed
induce
zebrafish.
This
study
identified
6000
2
h
as
optimal
condition
induction
Under
these
conditions,
notable
behavioral
changes
including
slow
movement,
abnormal
exploration
behavior
and
Novel
tank
test.
Furthermore,
model
demonstrated
oxidative
stress-related
markers
included
increased
levels
of
malondialdehyde,
decreased
glutathione,
activities
superoxide
dismutase
catalase,
inflammatory
IL-6,
IL-1β
TNF-α,
cell
infiltration
mild
edema
gill
tissue,
mirroring
clinical
pathophysiology
observed
patients.
innovative
zebrafish
not
only
offers
more
accurate
representation
but
also
provides
high-throughput
platform
discovery
pathogenesis
research.
Journal of Toxicology and Environmental Health,
Journal Year:
2023,
Volume and Issue:
86(11), P. 372 - 381
Published: April 26, 2023
Chloramine
T,
a
sodium
p-toluene
sulfonchloramide,
is
known
to
possess
wide
spectrum
of
biocidal
activity
and
employed
as
disinfectant
in
fish
farms
treat
bacterial
infections.
Although
T
may
effectively
combat
pathogens,
the
sublethal
lethal
effects
changes
acetylcholinesterase
(AChE)
remain
poorly
elucidated
using
Danio
rerio
(zebrafish)
embryos.
Zebrafish
considered
model
organism
for
toxicant
screening
research
exhibits
mammalian-like
physiological
responses
when
exposed
environmental
pollutants.
The
aim
this
study
was
(1)
determine
LC50
after
96
hr
exposure,
(2)
verify
on
developmental
morphology,
(3)
evaluate
AChE
zebrafish
exposure
performed
16,
32,
64,
128,
or
256
mg/L
concentrations.
mortality
values
were
143.05
±
3.11
130.97
7.4
at
24
hr,
respectively.
Data
demonstrated
delayed
hatching,
reduced
heartbeats,
cardiac
edema,
equilibrium
disruption
hatched
larvae
throughout
embryonic
development.
In
addition,
inhibited
64
128
treatment,
corroborating
sub-lethality
results
observed
embryo
development
demonstrating
an
larvae.
Fishes,
Journal Year:
2024,
Volume and Issue:
9(5), P. 164 - 164
Published: May 2, 2024
Data
variability
complicates
reproducibility
and
the
interpretation
of
experimental
results.
Different
animal
models
have
been
employed
to
decrease
enhance
power.
However,
variation
frequently
persists
among
within
strains/lines.
In
zebrafish
(Danio
rerio),
inbred
lines
(e.g.,
NHGRI-1)
derived
from
wild-type
produced
greatly
genetic
variation,
with
goal
providing
better
understanding
backgrounds
that
may
influence
outcome
studies
employing
such
lines.
We
hypothesized
variations
in
morphological
phenotypes
shaped
by
environmental
stressors
early
development
are
correlated
intrinsic
degree
compared
(yolk–chorion
ratio,
body
mass,
embryo
total
length,
condition
factor,
specific
growth
rate)
AB
NHGRI-1
as
a
function
their
responses
altered
temperature
oxygen
availability
during
first
7
days
post-fertilization.
Overall,
both
showed
similar
developmental
trajectories
for
yolk–chorion
length.
Additionally,
factor
rate
each
line,
regardless
hypoxia.
Importantly,
coefficient
variable
was
significantly
lower
than
larvae
151
187
assessed
endpoints.
Thus,
low-heterozygosity
line
can
be
useful
decreasing
inter-individual
stressors,
thereby
aiding
results
enhancing
reproducibility.
Deleted Journal,
Journal Year:
2024,
Volume and Issue:
7(2), P. 31 - 42
Published: June 5, 2024
Two
aquatic
ambient
factors,
viz.
Dissolved
Oxygen
(DO)
and
pH
were
selected
for
the
study
on
effect
of
oxidative
stress
in
liver
zebrafish.
The
zebrafish
exposed
to
four
DO
saturation
levels
(20-30%,
40-50%,
60-70%
80%
above)
five
(4.5-5.5,
5.5-6.5,
6.5-7.5,
7.5-8.5
8.5-9.5)
different
periods
(DO,
4h-16h
at
4hr
interval;
pH,
1h-4h
1
hr
interval).
antioxidants
studied
catalase,
glutathione
superoxide
dismutase
(SOD).
malondialdehyde
(MDA)
was
assessed
as
a
bioindicator
stress.
Results
showed
that
fish
suffered
from
all
(except
above,
control)
control).
However,
highest
generation
MDA
observed
20-30%
level
4.5-5.5
level.
Under
level,
12
h
2
treatments.
Accordingly,
antioxidant
enzymes
SOD
also
low
activity
treatment
h,
respectively.
It
evident
alkaline
(8.5-9.5),
although
altered
activity,
but
not
high
4.5-5.5,
compared
control.
