Biology, Journal Year: 2023, Volume and Issue: 12(2), P. 323 - 323

Published: Feb. 16, 2023

Toll-like receptors (TLRs) have been implicated in the regulation of various metabolism pathways, addition to their function innate immunity. Here, we investigate metabolic TLR2 a larval zebrafish system. We studied larvae from tlr2 mutant and wild type sibling controls an unchallenged normal developmental condition using transcriptomic metabolomic analyses methods. RNAseq was used evaluate differences between wild-type control found signature set 149 genes be significantly altered gene expression. The expression level several confirmed by qPCR analyses. Gene enrichment analysis (GSEA) revealed differential two genotypes related valine, leucine, isoleucine degradation glycolysis gluconeogenesis. Using 1H nuclear magnetic resonance (NMR) metabolomics, that glucose metabolites with were present at higher levels mutant. Furthermore, is mutants fluorometric assay. Therefore, shown TLR2, its immunity, has controlling during vertebrate development. functions are associated transcriptional enzymes involved could explain different glucose, lactate, succinate, malate

Language: Английский