Architecture of the cortical actomyosin network driving apical constriction inC. elegans DOI Creative Commons
Pu Zhang, Taylor N. Medwig-Kinney, Bob Goldstein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 1, 2023

Abstract Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In Drosophila ventral furrow, network has sarcomere-like architecture, with radially polarized actin filaments centrally enriched non-muscle myosin II activating kinase. To determine if this broadly conserved architecture constriction, we examined C. elegans gastrulation, in which two endodermal precursor cells internalize from surface embryo. Quantification protein localization showed neither NMY-2 nor myosin-activating kinase MRCK-1 at center apex. Further, visualization barbed- pointed-end capping proteins revealed do not exhibit radial polarization Taken together observations made other organisms, our results demonstrate diverse architectures used animal to accomplish constriction. Summary Through live-cell imaging endogenously-tagged proteins, Zhang, Medwig-Kinney, Goldstein show organized diffusely, contrast previously observed furrow.

Language: Английский

Canonical Wnt pathway modulation is required to correctly execute multiple independent cellular dynamic programs during cranial neural tube closure DOI Creative Commons

A. Bogart,

Eric Brooks

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Mechanical control of neural plate folding by apical domain alteration DOI Creative Commons
Miho Matsuda, Jan Rozman, Sassan Ostvar

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Dec. 20, 2023

Abstract Vertebrate neural tube closure is associated with complex changes in cell shape and behavior, however, the relative contribution of these processes to tissue folding not well understood. At onset Xenopus folding, we observed alternation apically constricted expanded cells. This apical domain heterogeneity was accompanied by biased orientation along anteroposterior axis, especially at plate hinges, required planar polarity signaling. Vertex models suggested that dispersed isotropically constricting cells can cause elongation adjacent Consistently, ectoderm, cell-autonomous constriction neighbor expansion. Thus, a subset may initiate bending, whereas ‘tug-of-war’ contest between force-generating responding reduces its shrinking body axis. mechanism an alternative anisotropic junctions are perpendicular We propose reflect polarity-dependent mechanical forces operating during folding.

Language: Английский

Citations

4
Architecture of the cortical actomyosin network driving apical constriction in C. elegans DOI Creative Commons
Pu Zhang, Taylor N. Medwig-Kinney, Bob Goldstein

et al.

The Journal of Cell Biology, Journal Year: 2023, Volume and Issue: 222(9)

Published: June 23, 2023

Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In Drosophila ventral furrow, network has sarcomere-like architecture, with radially polarized actin filaments centrally enriched non-muscle myosin II activating kinase. To determine if this broadly conserved architecture constriction, we examined C. elegans gastrulation, in which two endodermal precursor cells internalize from surface embryo. Quantification protein localization showed neither NMY-2 nor myosin-activating kinase MRCK-1 at center apex. Further, visualization barbed- pointed-end capping proteins revealed do not exhibit radial polarization Our results demonstrate apically constrict using mixed-polarity filament activator distributed throughout network. Taken together observations made other organisms, our diverse architectures used animal to accomplish constriction.

Language: Английский

Citations

3
The RhoGEF protein Plekhg5 self-associates via its PH domain to regulate apical cell constriction DOI

Ivan K. Popov,

Jiahui Tao, Chenbei Chang

et al.

Molecular Biology of the Cell, Journal Year: 2024, Volume and Issue: 35(10)

Published: Aug. 28, 2024

RhoGEFs are critical activators of Rho family small GTPases and regulate diverse biological processes, such as cell division tissue morphogenesis. We reported previously that the RhoGEF gene plekhg5 controls apical constriction bottle cells at blastopore lip during Xenopus gastrulation, but detailed mechanism action is not understood in depth. In this study, we show localization Plekhg5 cortex depends on its N-terminal sequences intact guanine nucleotide exchange activity, whereas C-terminal prevent ectopic protein to basolateral compartment. also reveal self-associates via PH domain, interaction leads functional rescue two mutants lack region factor respectively, trans. A point mutation domain corresponding a variant associated with human disease loss self-association failure mutant induce constriction. Taken together, our results suggest PH-mediated domain-mediated subcellular both crucial for function inducing

Language: Английский

Citations

0
Architecture of the cortical actomyosin network driving apical constriction inC. elegans DOI Creative Commons
Pu Zhang, Taylor N. Medwig-Kinney, Bob Goldstein

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 1, 2023

Abstract Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In Drosophila ventral furrow, network has sarcomere-like architecture, with radially polarized actin filaments centrally enriched non-muscle myosin II activating kinase. To determine if this broadly conserved architecture constriction, we examined C. elegans gastrulation, in which two endodermal precursor cells internalize from surface embryo. Quantification protein localization showed neither NMY-2 nor myosin-activating kinase MRCK-1 at center apex. Further, visualization barbed- pointed-end capping proteins revealed do not exhibit radial polarization Taken together observations made other organisms, our results demonstrate diverse architectures used animal to accomplish constriction. Summary Through live-cell imaging endogenously-tagged proteins, Zhang, Medwig-Kinney, Goldstein show organized diffusely, contrast previously observed furrow.

Language: Английский

Citations

0