The mechanobiology of biomolecular condensates DOI Creative Commons
Neus Sanfeliu-Cerdán, Michael Krieg

Biophysics Reviews, Journal Year: 2025, Volume and Issue: 6(1)

Published: March 1, 2025

The central goal of mechanobiology is to understand how the mechanical forces and material properties organelles, cells, tissues influence biological processes functions. Since first description biomolecular condensates, it was hypothesized that they obtain are tuned their functions inside cells. Thus, represent an intriguing playground for mechanobiology. idea condensates exhibit diverse adaptive highlights need different states respond external whether these responses linked physiological roles within cell. For example, liquids buffer dissipate, while solids store transmit stress, relaxation time a viscoelastic can act as frequency filter. Hence, liquid-solid transition condensate in force transmission pathway determine signals transduced in-between affecting differentiation, neuronal network dynamics, behavior stimuli. Here, we review our current understanding molecular drivers rigidity phase transitions set forth complex cellular environment. We will then summarize technical advancements were necessary insights into rich fascinating finally, highlight recent examples connection specific Our provide comprehensive summary field on cells harness regulate mechanics achieve

Language: Английский

Perspective: fluorescence lifetime imaging and single-molecule spectroscopy for studying biological condensates DOI Creative Commons
Maria Loidolt-Krüger

Methods in microscopy, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 23, 2025

Abstract Biological condensates, often formed via liquid-liquid phase separation (LLPS), are membraneless compartments organizing biochemical reactions. Recent advances have shifted the focus from identifying condensates to elucidating their dynamic biological functions, such as buffering concentrations, mediating reactions, and regulating signaling. These critical for cellular processes implicated in diseases like cancer neurodegeneration. Advanced microscopy techniques, including fluorescence lifetime imaging (FLIM), FLIM-FRET, correlation spectroscopy (FCS), enable quantitative, real-time investigations of condensate composition, dynamics, material properties, responses environmental stimuli live cells. This perspective highlights utility time-resolved single-molecule techniques shedding light on interactions with membranes, offering insights into physiology pathology.

Language: Английский

Citations

0
The mechanobiology of biomolecular condensates DOI Creative Commons
Neus Sanfeliu-Cerdán, Michael Krieg

Biophysics Reviews, Journal Year: 2025, Volume and Issue: 6(1)

Published: March 1, 2025

The central goal of mechanobiology is to understand how the mechanical forces and material properties organelles, cells, tissues influence biological processes functions. Since first description biomolecular condensates, it was hypothesized that they obtain are tuned their functions inside cells. Thus, represent an intriguing playground for mechanobiology. idea condensates exhibit diverse adaptive highlights need different states respond external whether these responses linked physiological roles within cell. For example, liquids buffer dissipate, while solids store transmit stress, relaxation time a viscoelastic can act as frequency filter. Hence, liquid-solid transition condensate in force transmission pathway determine signals transduced in-between affecting differentiation, neuronal network dynamics, behavior stimuli. Here, we review our current understanding molecular drivers rigidity phase transitions set forth complex cellular environment. We will then summarize technical advancements were necessary insights into rich fascinating finally, highlight recent examples connection specific Our provide comprehensive summary field on cells harness regulate mechanics achieve

Language: Английский

Citations

0