Plant-mediated rifampicin treatment of Bemisia tabaci disrupts but does not eliminate endosymbionts

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: Dec. 1, 2022

Abstract Whiteflies are among the most important global insect pests in agriculture; their sustainable control has proven challenging and new methods needed. Bacterial symbionts of whiteflies poorly understood potential target novel whitefly methods. harbour an obligatory bacterium, Candidatus Portiera aleyrodidarum, a diverse set facultative bacterial endosymbionts. Function microbial community is largely due to difficulty selective elimination without removal primary endosymbiont. Since discovery secondary endosymbionts, antibiotic rifampicin emerged as used tool for manipulation. Its effectiveness however much less clear, with contrasting reports on its effects endosymbiont community. The present study builds upon recent method application evaluates ability eliminate two endosymbionts (Rickettsia Arsenophnus). Our results show that reduces but does not any three Additionally, causes direct negative effect whiteflies, likely by disrupting mitochondria. Taken together, signify end era studies. Finally, we propose refinement current quantification data analysis which yields additional insights cellular metabolic scaling.

Language: Английский

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Susceptibility of Bemisia tabaci (Hemiptera: Aleyrodidae) Adult Populations to Imidacloprid in Georgia, USA

Journal of Entomological Science, Journal Year: 2024, Volume and Issue: 59(2)

Published: Jan. 3, 2024

Imidacloprid has been one of the most widely used insecticides for managing sweetpotato whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) in United States since U.S. Environmental Protection Agency first registered it 1994. A major control failure occurred state Georgia 2017 when B. tabaci;–induced economic losses horticultural and field crops surpassed approximately US$160 million. Vegetable growers have historically imidacloprid management, which likely led to insecticide failures whiteflies spring vegetables. Despite this, only a single site documentation resistance adults from 2007 exists, making current status unknown. Thus, is that an ongoing risk imidacloprid-resistant tabaci; infestations. No multicounty, extensive survey dose response exists Georgia. Therefore, adult mortality bioassay range concentrations was evaluate populations several counties South Georgia, where these occurred. This included maximum concentration representing highest labeled rate. Dose not uniform across locations, with areas displaying unexpected susceptibility imidacloprid. Median lethal (LC50s) ranging 0.02 196.05 mg active ingredient per liter were substantially lower than reference Florida population. baseline information critical future evaluations this management programs.

Language: Английский

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Modified Maximum Dose Bioassay for Assessing Insecticide Response in Field Populations of Bemisia tabaci (Hemiptera: Aleyrodidae)1

Journal of Entomological Science, Journal Year: 2024, Volume and Issue: 59(4)

Published: April 19, 2024

The sweetpotato whitefly, Bemisia tabaci (Gennadius) MEAM1 (Hemiptera: Aleyrodidae), continues to be a major pest of vegetable cultivation in Georgia, USA. Field-by-field surveying is an effective approach determining the susceptibility status B. population insecticide. During 2020–2022, modified maximum dose bioassay method was tested characterize insecticide response field populations several commonly used insecticides for whitefly management Tift Co., GA, and surrounding areas. A rapid these evaluations that allowed assessments before spray applications reduce adult life stage this species. results were produced within 24-h following root drench period. Our survey suggests neonicotinoids dinotefuran flupyradifurone most from Insecticide Resistance Action Committee (IRAC) group 4A. Cyantraniliprole also effective, with 88 86% mortality exposure high (maximum) low doses, respectively. Conversely, levels control using another diamide, cyclaniliprole, notably lower. Adding provided early indication inefficient product potentially indicating increase resistance. Specifically, significant difference between doses dose–response curve had shifted toward resistance development each population. proposed meant systemic offer quick responses on adults. use efficient will improve prioritizing or rotation program.

Language: Английский

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Role of eggplant trichome in whitefly oviposition and its relevance to biological control under greenhouse conditions

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 30, 2024

Language: Английский

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Attraction of Sweet Potato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), and Two Generalist Predators to Green Leaf Volatile Compounds

Insects, Journal Year: 2024, Volume and Issue: 15(10), P. 750 - 750

Published: Sept. 28, 2024

Traditionally, olfaction was thought to play a minor role in the behavioral ecology of sweet potato whitefly, Bemisia tabaci (Gennadius). However, recent research is uncovering significant potential for whitefly management based on olfaction. Incorporating chemical attractants with standard programs could significantly improve control. The integration biological control exceptionally promising. Therefore, response B. and two generalist predators green leaf volatiles (E)-2-hexenal, (Z)-3-hexenal, (Z)-3-hexenyl acetate, (Z)-3-hexe-1-ol were investigated Y-tube olfactometers. Three four resulted attraction female tabaci. Blend optimization indicated two-chemical blend be most attractive In addition, this Macrolophus praeclarus (Distant) but did not elicit from either male or Delphastus catalinae (Horn). further developed as lure attract its predator, M. praeclarus.

Language: Английский

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RNA Interference in the Study of Gene Functions in Aphids and Other Hemipterans and the Fight against Their Harmful Representatives

Biology Bulletin Reviews, Journal Year: 2024, Volume and Issue: 14(S3), P. S351 - S375

Published: Dec. 1, 2024

Language: Английский

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Vector-mediated viral exchange between crops and weedy plants

Journal of Pest Science, Journal Year: 2023, Volume and Issue: 97(1), P. 155 - 171

Published: June 28, 2023

Language: Английский

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RNA interference in the study of gene functions in aphids and other hemipterans and the fight against their harmful representatives

Plant Protection News, Journal Year: 2023, Volume and Issue: 106(1), P. 26 - 48

Published: May 24, 2023

RNA interference (RNAi) is an important mechanism that protects cells of eukaryotic organisms from foreign genetic information and regulates physiological processes. Discovery RNAi development the methods for targeted suppression expression individual genes have opened up wide opportunities elucidating gene functions solving many problems in biology, medicine, plant protection. This approach can contribute to new effective, highly specific, environmentally safe ways control harmful insects. Several examples practical use combating chewing pests been already reported. Due their phloem-feeding, as well delivering constructs into body, insects with piercing-sucking mouthparts – aphids, bugs, other hemipterans deserves separate consideration. review discusses criteria selecting target issues functional activity solved by RNAi, double-stranded small RNAs various Hemiptera, non-target effects prevent them, possible outcomes using this hemipteran agricultural plants vectors dangerous human animal diseases.

Language: Английский

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A new pest suction machine to control Bemisia tabaci (Hemiptera: Aleyrodidae) in tomato greenhouses

Journal of Economic Entomology, Journal Year: 2024, Volume and Issue: 117(4), P. 1616 - 1622

Published: May 17, 2024

Abstract The sweetpotato whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) is challenging to control using chemical pesticides owing its resistance many insecticides. Thus, there has been an increasing demand for alternative measures. this study evaluated the efficacy of a newly designed pest suction machine manage whiteflies on tomato plants (Solanum lycopersicum L.) (Solanales: Solanaceae) in greenhouses over 2 seasons. comprised battery-powered cart with mounted unit, ultrasonic device, and green lights. Ultrasonic irradiation provided non-contact vibration, facilitating movement adult away from plants, lights attracted them device. This combination effectively captured adults, even weak force, saving electricity consumption. was further by measuring number adults caught nymphs remaining leaves. population considerably lower treated blocks than non-treated autumn trial. reduced density without pesticides. Although lot optimizations would be required, additional strategy that may incorporated integrated management greenhouse plants.

Language: Английский

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Involvement of the cytochrome P450 genes CYP6DW3 and CYP4C64 in afidopyropen resistance in Bemisia tabaci Mediterranean (Q Biotype)

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

The tobacco whitefly, Bemisia tabaci, is a notorious pest affecting various crops globally, and it exhibits high levels of resistance to insecticides. Afidopyropen recently commercialized pyropene insecticide for B. tabaci control with selectivity novel mode action. We previously identified level afidopyropen in field-collected population after selection the lab, named HD-Afi strain. In present study, minimal cross-resistance strain was found between other common chemical agents. However, P450 enzyme activity 2.18 times susceptible HD-S. Expression analysis revealed that two 12 candidate genes, namely CYP6DW3 CYP4C64, were significantly up-regulated HD-Afi. Silencing CYP4C64 by RNA interference (RNAi) substantially increased susceptibility whitefly adults, confirming their involvement resistance. Homology modeling molecular docking analyses demonstrated stable binding free energies -6.87 -6.11 kcal mol-1, respectively. findings this study suggest induction facilitates detoxification, contributing development tabaci.

Language: Английский

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