OsWHY1/OsTRXz/OsMORFs complex is essential for RNA modification and early chloroplast development in rice DOI Creative Commons

Xiangzi Zheng,

Qingzheng Lu,

Yuling Luo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract WHIRLY (WHY) proteins are single-stranded DNA/RNA-binding that play multifaceted roles in various plant species. The regulatory mechanisms of WHY rice remains blank. Here we demonstrate OsWHY1 is required for early chloroplast development. CRISPR/Cas9-generated oswhy1 knockout lines displayed albino seedling phenotypes, abnormal structure and comprised redox balance leaves. interacts with multiple plastid proteins, including the thioredoxin OsTRXz two organellar RNA editing factors (OsMORF8 OsMORF9) chloroplasts. Accordingly, several genes dependent on plastid-encoded polymerase (PEP) mutants were significantly depressed at both transcript protein levels. rps14 transcripts splicing rpl2, along their expression, defective mutants. exhibited RNA-binding activity, specifically binding to rpl2 precursor RNAs, which underscores its role as a post-transcriptional regulator essential normal synthesis Loss-of- function either or OsMORF9 disrupted H 2 O homeostasis, processing , suggesting OsWHY1-OsTRXz-OsMORFs module vital maintaining stability integrity through activity recruiting OsMORFs ensure proper modification. One sentence summary integral development

Language: Английский

Functional Characterization of the PoWHY1 Gene from Platycladus orientalis and Its Role in Abiotic Stress Tolerance in Transgenic Arabidopsis thaliana DOI Creative Commons
Chun‐Quan Ou,

Zhiyu Dong,

Xudong Zheng

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 218 - 218

Published: Jan. 14, 2025

The frequent occurrence of extreme weather conditions in the world has brought many unfavorable factors to plant growth, causing growth and development plants be hindered even leading death, with abiotic stress hindering metabolism due severe uncontrollability. WHY1 transcription factor plays a critical role regulating gene expression plants, influencing chlorophyll biosynthesis, development, as well responses environmental stresses. important PoWHY1 adaptation become hot research topic. However, mechanism Platycladus orientalis under is still unclear. Here, was analyzed bioinformatically using P. study material, against Arabidopsis thaliana verified transgenic technology. It found that overexpression increased seed germination, decreased malondialdehyde accumulation, proline content, delayed senescence process salt stress. levels JAZ1, LOX1, ABI1, ABI2 were decreased, while RAB18, APX1, GSTF6, DREB2A increased, indicating enhanced tolerance A. thaliana. Furthermore, also drought From above results, it can concluded maintaining high leaves improve their adaptability. results provide scientific basis for understanding function lay foundation further on gene.

Language: Английский

Citations

0
The transcription factor TaWHY2-6A acts as a positive regulator in response to drought tolerance in transgenic plants DOI
Yang Yu,

Conglei Wang,

Xiao Zhang

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2025, Volume and Issue: unknown, P. 151580 - 151580

Published: March 1, 2025

Language: Английский

Citations

0
Genome-Wide Identification of the Whirly Gene Family and Its Potential Function in Low Phosphate Stress in Soybean (Glycine max) DOI Open Access
Zhimin Li,

Xuhao Zhai,

Lina Zhang

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(7), P. 833 - 833

Published: June 25, 2024

The

Language: Английский

Citations

2
OsWHY1/OsTRXz/OsMORFs complex is essential for RNA modification and early chloroplast development in rice DOI Creative Commons

Xiangzi Zheng,

Qingzheng Lu,

Yuling Luo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract WHIRLY (WHY) proteins are single-stranded DNA/RNA-binding that play multifaceted roles in various plant species. The regulatory mechanisms of WHY rice remains blank. Here we demonstrate OsWHY1 is required for early chloroplast development. CRISPR/Cas9-generated oswhy1 knockout lines displayed albino seedling phenotypes, abnormal structure and comprised redox balance leaves. interacts with multiple plastid proteins, including the thioredoxin OsTRXz two organellar RNA editing factors (OsMORF8 OsMORF9) chloroplasts. Accordingly, several genes dependent on plastid-encoded polymerase (PEP) mutants were significantly depressed at both transcript protein levels. rps14 transcripts splicing rpl2, along their expression, defective mutants. exhibited RNA-binding activity, specifically binding to rpl2 precursor RNAs, which underscores its role as a post-transcriptional regulator essential normal synthesis Loss-of- function either or OsMORF9 disrupted H 2 O homeostasis, processing , suggesting OsWHY1-OsTRXz-OsMORFs module vital maintaining stability integrity through activity recruiting OsMORFs ensure proper modification. One sentence summary integral development

Language: Английский

Citations

0