Communications Biology,
Journal Year:
2023,
Volume and Issue:
6(1)
Published: March 16, 2023
Abstract
Bacterial
antibiotic
resistance
is
a
global
health
concern
of
increasing
importance
and
intensive
study.
Although
biofilms
are
common
source
infections
in
clinical
settings,
little
known
about
the
development
within
biofilms.
Here,
we
use
experimental
evolution
to
compare
selection
mutations
planktonic
biofilm
Escherichia
coli
populations
exposed
clinically
relevant
cycles
lethal
treatment
with
aminoglycoside
amikacin.
Consistently,
sbmA
,
encoding
an
inner
membrane
peptide
transporter,
fusA
essential
elongation
factor
G,
rapidly
selected
biofilms,
but
not
cells.
This
due
combination
enhanced
mutation
rate,
increased
adhesion
capacity
protective
biofilm-associated
tolerance.
These
results
show
that
environment
favors
rapid
provide
new
insights
into
dynamic
PLoS Biology,
Journal Year:
2021,
Volume and Issue:
19(11), P. e3001442 - e3001442
Published: Nov. 9, 2021
The
archaeal
tailed
viruses
(arTV),
evolutionarily
related
to
double-stranded
DNA
(dsDNA)
bacteriophages
of
the
class
Caudoviricetes
,
represent
most
common
isolates
infecting
halophilic
archaea.
Only
a
handful
these
have
been
genomically
characterized,
limiting
our
appreciation
their
ecological
impacts
and
evolution.
Here,
we
present
37
new
genomes
haloarchaeal
virus
isolates,
more
than
doubling
current
number
sequenced
arTVs.
Analysis
all
63
available
complete
arTVs,
which
propose
classify
into
14
families
3
orders,
suggests
ancient
divergence
bacterial
points
an
extensive
sharing
genes
involved
in
metabolism
counterdefense
mechanisms,
illuminating
strategies
virus–host
interactions
with
bacteriophages.
Coupling
comparative
genomics
host
range
analysis
on
broad
panel
species
uncovered
4
distinct
groups
viral
tail
fiber
adhesins
controlling
expansion.
survey
metagenomes
using
hallmark
that
global
architecture
arTV
community
is
shaped
through
recurrent
transfers
between
different
biomes,
including
hypersaline,
marine,
anoxic
environments.
Nature Communications,
Journal Year:
2022,
Volume and Issue:
13(1)
Published: March 21, 2022
Hadal
trenches
are
the
deepest
and
most
remote
regions
of
ocean.
The
11-kilometer
deep
Challenger
Deep
is
least
explored
due
to
technical
challenges
sampling
hadal
depths.
It
receives
organic
matter
heavy
metals
from
overlying
water
column
that
accumulate
differently
across
its
V-shaped
topography.
Here,
we
collected
sediments
slope
bottom-axis
enable
insights
into
in
situ
microbial
communities.
Analyses
586
metagenome-assembled
genomes
retrieved
37
metagenomes
show
distinct
diversity
metabolic
capacities
between
sites.
26%
prokaryotic
16S
rDNA
reads
were
novel,
with
novelty
increasing
sediment
These
predominantly
heterotrophic
microbes
can
recycle
macromolecules
utilize
simple
complex
hydrocarbons
as
carbon
sources.
Metagenome
metatranscriptome
data
support
reduction
biotransformation
arsenate
for
energy
gain
present
a
two-fold
greater
accumulation
arsenic
compared
non-hadal
Complete
pathways
anaerobic
ammonia
oxidation
identified
recovered
Our
results
expand
knowledge
microbially-mediated
elemental
cycling
sediments,
reveal
differences
distribution
processes
involved
nitrogen
loss
trench.
Communications Biology,
Journal Year:
2023,
Volume and Issue:
6(1)
Published: March 16, 2023
Abstract
Bacterial
antibiotic
resistance
is
a
global
health
concern
of
increasing
importance
and
intensive
study.
Although
biofilms
are
common
source
infections
in
clinical
settings,
little
known
about
the
development
within
biofilms.
Here,
we
use
experimental
evolution
to
compare
selection
mutations
planktonic
biofilm
Escherichia
coli
populations
exposed
clinically
relevant
cycles
lethal
treatment
with
aminoglycoside
amikacin.
Consistently,
sbmA
,
encoding
an
inner
membrane
peptide
transporter,
fusA
essential
elongation
factor
G,
rapidly
selected
biofilms,
but
not
cells.
This
due
combination
enhanced
mutation
rate,
increased
adhesion
capacity
protective
biofilm-associated
tolerance.
These
results
show
that
environment
favors
rapid
provide
new
insights
into
dynamic
