Microfluidics: The future of sperm selection in assisted reproduction

Andrology, Journal Year: 2023, Volume and Issue: 12(6), P. 1236 - 1252

Published: Dec. 26, 2023

Obtaining functional sperm cells is the first step to treat infertility. With ever-increasing trend in male infertility, clinicians require access effective solutions that are able single out most viable spermatozoa, which would max chance for a successful pregnancy. The new generation techniques selection involve microfluidics, offers laminar flow and low Reynolds number within platforms can provide unprecedented opportunities selection. Previous studies showed microfluidic novel approach this challenge since then researchers across globe have attacked problem from multiple angles.

Language: Английский

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Advanced Sperm Selection Techniques for Assisted Reproduction

Journal of Personalized Medicine, Journal Year: 2024, Volume and Issue: 14(7), P. 726 - 726

Published: July 4, 2024

Male infertility accounts for approximately 40% of cases. There are many causes male infertility, including environmental factors, age, lifestyle, infections, varicocele, and cancerous pathologies. Severe oligozoospermia, cryptozoospermia, azoospermia (obstructive non-obstructive) identified as severe factor once considered conditions sterility. Today, in vitro fertilization (IVF) techniques the only treatment strategy cases which new methodologies have been developed manipulation spermatozoa to achieve increase success rates. This review is an update techniques, particular sperm selection, emphasizing clinical case-specific methodology. The IVF process related diagnosis, appropriate choice treatment, effective preparation selection. In fact, selecting best guarantee optimal paternal heritage means increasing blastulation, implantation, ongoing pregnancy live birth rates, resulting greater techniques.

Language: Английский

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Porcine sperm bind to an oviduct glycan coupled to glass surfaces as a model of sperm interaction with the oviduct

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 8, 2025

During transport through the oviduct, sperm interact with epithelial cells by attaching to specific glycans, a mechanism believed select and prolong their viability. An in vitro model of sperm-oviduct interactions was developed, consisting glass surface (either slide or coverslip) which an oviduct glycan (sulfated Lewis X trisaccharide; suLeX) is coupled. The ability porcine attach suLeX-surfaces detach response progesterone mature cumulus-oocyte complexes (COCs) validated. suLeX-coverslip adapted for fertilization (IVF), termed glycan-IVF, allowing first bind suLeX before transferring COCs. glycan-IVF method produced percentage fertilized oocytes comparable that conventional IVF (75.1 vs. 72.0%). Finally, maintain fertilizing over time assessed. After 24 h incubation, bound sustained, compared unmodified coverslips (12.0 1.0%, p < 0.05). Furthermore, polyspermic zygotes reduced (17.7 41.3%, This study validated studying interactions, potential applications assisted reproductive technologies.

Language: Английский

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IVF – through the looking glass

Reproductive BioMedicine Online, Journal Year: 2025, Volume and Issue: 50(4), P. 104835 - 104835

Published: April 1, 2025

Language: Английский

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Assessment of sperm DNA integrity and ICSI outcome after sperm cryopreservation

Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Journal Year: 2025, Volume and Issue: 77(3)

Published: Jan. 1, 2025

ABSTRACT The association between suboptimal sperm parameters and DNA damage in mature spermatozoa indicates that some individuals may have intrinsic issues with spermatogenesis. objective of this study is to evaluate the impact cryopreservation reactive oxygen species (ROS) on stability, as assessed by Sperm Chromatin Structure Assay (SCSA) motility viability, ICSI Result. Participants contacted Fertility Clinic provided written informed consent. All data were subsequently handled processed a confidential anonymous manner. There was source 300 participants (600 semen samples) study, divided into 3 groups. female partners underwent ICSI, (some oocytes injected fresh sperms others cryopreserved for same patient (liquid nitrogen at−196°C, accordance conventional protocols). Cryopreservation from fertile men does not seem adversely affect integrity either or prepared samples. However, morphology both samples significantly compromised process. No evidence cryopreserving healthy males would compromise genetic material laboratory-prepared

Language: Английский

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Sperm in the Mammalian Female Reproductive Tract: Surfing Through the Tract to Try to Beat the Odds

Annual Review of Animal Biosciences, Journal Year: 2023, Volume and Issue: 12(1), P. 301 - 319

Published: Oct. 31, 2023

Mammalian sperm are deposited in the vagina or cervix/uterus at coitus artificial insemination, and fertilizing move through female reproductive tract to ampulla of oviduct, site fertilization. But destination most is not oviduct. Most carried by retrograde fluid flow vagina, phagocytosed, and/or do pass barriers on pathway The that reach fertilization exceptions winners one stringent selection processes nature. This review discusses challenges encounter how few overcome them. goal must navigate viscoelastic fluid, swim vigorously cooperatively along walls tract, avoid innate immune system, respond potential cues direct their movement.

Language: Английский

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Running the gauntlet: challenges to genome integrity in spermiogenesis

Nucleus, Journal Year: 2024, Volume and Issue: 15(1)

Published: April 9, 2024

Species' continuity depends on gametogenesis to produce the only cell types that can transmit genetic information across generations. Spermiogenesis, which encompasses post-meiotic, haploid stages of male gametogenesis, is a process leads formation sperm cells well-known for their motility. Spermiogenesis faces three major challenges. First, after two rounds meiotic divisions, genome lacks repair templates (no sister chromatids, no homologous chromosomes), making it incredibly vulnerable any genomic insults over an extended time (typically days-weeks). Second, becomes transcriptionally silent, difficult respond new perturbations as spermiogenesis progresses. Third, histone-to-protamine transition, essential package genome, counterintuitively involves DNA break formation. How handles these challenges remains poorly understood. In this review, we discuss each challenge and intersection with biology protamines. Finally, implication protamines in evolution.

Language: Английский

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A high-resolution spatial map of cilia-associated proteins based on characterization of the human fallopian tube-specific proteome

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 9, 2024

Abstract Molecular changes in the fallopian tubes (FT) play a crucial role development of cancer and reproductive disorders. Here, we aimed to map key FT proteins on single-cell level utilizing an integrated transcriptomics proteomics approach. Based RNA-seq, 315 genes were identified as elevated FT, out which majority associated with motile cilia function. An in-depth spatial characterization was performed for 130 these other human tissues cilia, localizing different subcellular structures ciliated cells. The specificity cells validated RNA-seq in-situ mass-spectrometry data. Our approach enabled us identify 34 novel cilia-related lacking previous evidence protein level, well several not described context biology. high-resolution aids further disentangling pathways involved infertility diseases linked cilia-specific functions.

Language: Английский

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Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 104(1), P. 151471 - 151471

Published: Dec. 17, 2024

After insemination, a subpopulation of sperm reaches the oviducts and binds to isthmic epithelial cells form "sperm reservoir". Our objective was explore role annexin A5 (ANXA5), protein that with high affinity phosphatidylserine (PS), in formation reservoir pigs. Phosphatidylserine detected on head approximately 10 % boar at ejaculation. Porcine ANXA5 immunodetected strong signal luminal cilia isthmus derived spheroids (IES). Exogenous PS between 0.01 0.1 µg/mL recombinant porcine (rpANXA5) above inhibited binding IES without reducing motility. Pre-incubation sperm, but not IES, rpANXA5 IES. Under capacitating conditions, proportion live exposure ability bind increased within 30 min. Conversely, acrosome reaction decreased prevented In conclusion, membrane remodelling during capacitation enhanced motile resulting interaction ciliary spheroids. These findings support for PS-ANXA5 mammalian females.

Language: Английский

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D-Mannose-Mediated metabolic pathways sustain the molecular signatures of sperm function and fertilization

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

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