Prolonged Immunomodulator Delivery Boosts Monocyte Exosome Secretion and Elevates Cathelicidin/LL-37 Content DOI
Dongxin Ma, Yajuan Su, Navatha Shree Sharma

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 799 - 810

Published: Dec. 19, 2024

Human cathelicidin LL-37 offers significant benefits to the immune system and in treating various diseases, but its therapeutic potential is hindered by low activity instability physiological environments. Here, we introduce a strategy boost levels exosomes derived from THP-1 monocytes incubating cells with electrospun nanofibers containing immunomodulators (e.g., 1α, 25-dihydroxyvitamin D3 VID400). Notably, incubation immunomodulator-loaded not only increased content also significantly enhanced production of engineered exosomes. Moreover, these demonstrated multiple biological activities, including promoting skin cell proliferation migration, enhancing endothelial tube formation, exhibiting antibacterial properties. Collectively, this study presents an approach increasing both yield their content, potentially offering promising option for wound healing, tissue regeneration, infectious disease treatment.

Language: Английский

Exosome-carried miR-1248 from adipose-derived stem cells improves angiogenesis in diabetes-associated wounds DOI
Xichao Jian,

Jiansu Han,

Xin Liu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 297, P. 139822 - 139822

Published: Jan. 13, 2025

Language: Английский

Citations

1
Recent advances and future prospects of engineered exosomes as advanced drug and gene delivery systems DOI
Waleed Y. Rizg, Majed Alghamdi, Sherif El Saadany

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2025, Volume and Issue: 106, P. 106696 - 106696

Published: Feb. 6, 2025

Language: Английский

Citations

1
Advances of exosomes in diabetic wound healing DOI Creative Commons
Wenqi Jin, Yi Li,

Meirong Yu

et al.

Burns & Trauma, Journal Year: 2025, Volume and Issue: 13

Published: Jan. 1, 2025

Poor wound healing is a refractory process that places an enormous medical and financial burden on diabetic patients. Exosomes have recently been recognized as crucial players in the of lesions. They excellent stability, homing effects, biocompatibility, reduced immunogenicity novel cell-free therapies. In addition to transporting cargos target cells enhance intercellular communication, exosomes are beneficial nearly every phase healing. participate modulating inflammatory response, accelerating proliferation reepithelization, increasing angiogenesis, regulating extracellular matrix remodeling. Accumulating evidence indicates hydrogels or dressings conjunction with can prolong duration exosome residency wounds. This review provides overview mechanisms, delivery, clinical application, engineering, existing challenges use repair. We also propose future directions for biomaterials incorporating exosomes: 2D 3D scaffolds, loaded healing-promoting gases, intelligent biomaterials, prospect systematic application exosomes. These findings may might shed light treatments enlighten some studies improve quality life among diabetes

Language: Английский

Citations

1
Therapeutic potential of exosomal lncRNAs derived from stem cells in wound healing: focusing on mesenchymal stem cells DOI Creative Commons

Ali Morabbi,

Mohammad Karimian

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 11, 2025

The self-renewal ability and multipotency of stem cells give them great potential for use in wound healing. Stem cell-derived exosomes, owing to their close biological resemblance parent cells, offer a more efficient, safer, economical approach facilitating cellular communication interactions within different environments. This makes particularly valuable the treatment both acute chronic wounds, such as lacerations, burns, diabetic ulcers. Long non-coding RNAs (lncRNAs) enclosed one leading actors these extracellular microvesicles, through interaction with miRNAs regulation various signaling pathways involved inflammation, angiogenesis, cell proliferation, migration, could heal wounds. Exosome-derived lncRNAs from facilitate matrix remodeling between macrophages fibroblasts. Moreover, alongside regulating expression inflammatory cytokines, controlling reactive oxygen species levels, enhancing autophagic activity, they also modulate immune responses support Regulating genes related by increasing blood supply accelerating delivery essential substances environment, is another effect exosomal derived These can enhance skin healing homeostasis, proliferation differentiation wound-healing process, fibroblast viability migration injury site. Ultimately, exosome-derived novel insights into molecular mechanisms underlying improved They pave way therapeutic strategies, fostering further research better future. Meanwhile, exosomes mesenchymal due exceptional regenerative properties, well have emerged innovative tools review article aims narrate roles focus on cells.

Language: Английский

Citations

0
A systematic review and meta-analysis to investigate the effectiveness of exosome for diabetic wounds DOI Creative Commons
Lan Chen, Jia Liu, Y L He

et al.

Journal of Tissue Viability, Journal Year: 2025, Volume and Issue: 34(3), P. 100917 - 100917

Published: April 17, 2025

Exosomes, small endosome-derived membrane vesicles, have shown significant potential as wound healing therapies. However, translating experimental research into commercially available treatments remains a challenge. This systematic review and meta-analysis provide comprehensive evaluation of the current on exosome-based A search PubMed Google Scholar was conducted to identify full-text articles published between 2010 February 2024 mammalian-derived exosomes in healing. Of 138 identified studies, 19 met inclusion criteria for meta-analysis. Exosome-based therapies were found enhance by promoting angiogenesis, re-epithelialization, collagen deposition while reducing scar formation. this area is highly variable, with differences cell sources, biomaterials, delivery methods. Further comparative studies are needed optimize cellular systems, biomaterials. The reliance rodent models limitation, progress toward large-scale testing more advanced vivo has been slow. Addressing these challenges crucial clinical translation scalable, viable treatments.

Language: Английский

Citations

0
Antimicrobial Dual-Crosslinked Hydrogel Synergizes Bioengineered Extracellular Vesicles for Enhanced Diabetic Wound Healing DOI Creative Commons

Yikun Ju,

Yang Pu, Xiangjun Liu

et al.

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101870 - 101870

Published: May 1, 2025

Language: Английский

Citations

0
Analysis of clinical characteristics in patients with diabetic foot ulcers undergoing amputation and establishment of a nomogram prediction model DOI Creative Commons
Tiantian Xu,

Leiwing Hu,

Banglin Xie

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 14, 2024

To assess the accuracy of a nomogram for predicting risk lower-extremity amputation (LEA) in individuals with diabetic foot ulcers (DFUs). We retrospectively analyzed data from 144 patients DFUs at Department Orthopedics First Affiliated Hospital Nanchang University, collected between January 2020 and December 2023. Univariate analysis determined primary predictive factors amputation, followed by single multifactor logistic regression analyses to indentify independent factors. These were utilized develop prediction model using R4.3.3, was created. Its performance verified receiver operating characteristic (ROC), corrected calibration, clinical decision curves. Twelve identified 20 variables, including age, Wagner grades, peripheral angiopathy diabetes (PAD), chronic kidney disease(CKD), C-reactive protein(CRP) number blood sugar abnormalities(BSA) etc. Multivariate logical illustrated that PAD, CRP, CKD, BSA The area under curve (AUC) ROC 0.967, revised calibration demonstrated strong fitting ability. This is valuable tool screening LEA preventing DFU progressing into amputation.

Language: Английский

Citations

2
Application of 3D printing in the treatment of diabetic foot ulcers: current status and new insights DOI Creative Commons
Xinrui Li, Ai Xin, Bo Wang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 13, 2024

Background and Aims Diabetic foot ulcers (DFUs) are a serious complication of diabetes mellitus (DM), affecting around 25% individuals with DM. Primary treatment DFU involves wound off-loading, surgical debridement, dressings to provide moist environment, vascular assessment, appropriate antibiotics through multidisciplinary approach. Three-dimensional (3D) printing technology is considered an innovative tool for the management DFUs. The utilization 3D in modernization traditional methods exploration new techniques. This review discusses recent advancements application care, development personalized interventions Methods We searched electronic database years 2019–2024. Studies related use were included. Results A total 25 identified articles based on search citation network analysis. After removing duplicates, 18 remained, three that did not meet inclusion criteria removed after reading title/abstract. 97 relevant included during references. In total, 112 Conclusion offers unparalleled advantages, particularly realm treatment. amalgamation has yielded favorable outcomes decelerating progression DFUs facilitating healing. However, there limited body research regarding domain

Language: Английский

Citations

1
Prolonged Immunomodulator Delivery Boosts Monocyte Exosome Secretion and Elevates Cathelicidin/LL-37 Content DOI
Dongxin Ma, Yajuan Su, Navatha Shree Sharma

et al.

ACS Applied Materials & Interfaces, Journal Year: 2024, Volume and Issue: 17(1), P. 799 - 810

Published: Dec. 19, 2024

Human cathelicidin LL-37 offers significant benefits to the immune system and in treating various diseases, but its therapeutic potential is hindered by low activity instability physiological environments. Here, we introduce a strategy boost levels exosomes derived from THP-1 monocytes incubating cells with electrospun nanofibers containing immunomodulators (e.g., 1α, 25-dihydroxyvitamin D3 VID400). Notably, incubation immunomodulator-loaded not only increased content also significantly enhanced production of engineered exosomes. Moreover, these demonstrated multiple biological activities, including promoting skin cell proliferation migration, enhancing endothelial tube formation, exhibiting antibacterial properties. Collectively, this study presents an approach increasing both yield their content, potentially offering promising option for wound healing, tissue regeneration, infectious disease treatment.

Language: Английский

Citations

0