Multi-Residue Detection of Eight Glucocorticoids by Nano-Au/Fluticasone Propionate Electrochemical Immunosensor DOI Creative Commons
Guozheng Zhao

Molecules, Journal Year: 2023, Volume and Issue: 28(18), P. 6619 - 6619

Published: Sept. 14, 2023

Based on an indirect competitive method, a novel nano-Au/fluticasone propionate electrochemical immunosensor was successfully fabricated by combining the nanoscale effect, superior conductivity of nano-Au, stable Au−S chemical bond as well strong interaction between glucocorticoid and receptor, which used to simultaneously detect eight kinds glucocorticoids. The modified immunosensors’ properties were explored means cyclic voltammetry (CV) method impedance spectroscopy (EIS) measurements. Two factors (glucocorticoid receptor concentration, incubation time) studied in order obtain optimal results. presents attractive performance with wide linear range (between 0.1 1500 ng⋅mL−1) low detection limit 0.057 0.357 ng⋅mL−1), realizing rapid multi-residue large class glucocorticoids (hydrocortisone, triamcinolone) detected actual skincare samples, obtained satisfactory

Language: Английский

Adrenal response to competitive singing: glucocorticoid metabolites in male Saltator similis (Aves, Thraupidae) DOI Creative Commons

Carolina Lorieri-Vanin,

Heriberto Barbosa-Moyano, Clarice Diniz Alvarenga

et al.

Conservation Physiology, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 1, 2025

Abstract Song competitions involving passerines, such as the Green-winged Saltator (Saltator similis), are legally permitted in Brazil and attract widespread participation. This study aimed to assess adrenal response male S. similis by comparing glucocorticoid metabolite (GCM) levels uro-faecal extract samples collected during three with those from a rest day (3 days before competition, D-3), competition (D-1), of (D0) after (D1). Simultaneously, we examined potential variation GCM among other males not engaged song but subjected ex situ conditions much like participating males. were measured using direct enzyme immunoassay (EIA, CJM006), which was physiologically (ACTH challenge) analytically validated (parallelism, accuracy precision tests) for species under study. The results indicated that average concentration lower group (33.43 ± 22.09 ng/g) compared control (70.09 29.45 ng/g; P = 0.01). However, concentrations spiked significantly on (D0: 38.29 26.12 (D-3: 28.64 17.86 0.02), suggesting acute stress response. Given elevated observed competitions, further research is necessary confirm welfare these birds explore long-term effects stressors.

Language: Английский

Citations

0
Fecal Glucocorticoid Metabolite Responses of Brown Kiwi (Apteryx mantelli) to Ambassador Program Participation and Translocation: Implications for Captive Management and Welfare DOI Creative Commons

Kathleen Brader,

Natalia A. Prado, Janine L. Brown

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(8), P. 1156 - 1156

Published: April 17, 2025

The brown kiwi (Apteryx mantelli) is a flightless, nocturnal bird native to New Zealand and classified as “At Risk” due predation from domestic dogs ferrets. In the U.S., have been managed under AZA Animal Population Management Studbook Program since 2006, with Smithsonian National Zoological Park (NZP) Conservation Biology Institute (SCBI) maintaining species 1968. However, because they are nocturnal, not good exhibit animals often difficult for zoo visitors observe during day. To address this, NZP launched “Meet-A-Kiwi” ambassador program in 1989 engage public raise awareness. ran successfully 28 years until 2017, when renovations closed Bird House at Washington, DC, birds were moved Front Royal, VA campus. Over time, concerns about suitability of arose do imprint on people. This study assessed effects outreach events, housing, relocation adrenal activity five (four males, one female) by measuring fecal glucocorticoid metabolite (fGCM) concentrations, physiological stress indicator, March October 2016. Two males participated (ambassadors), while two female did (controls). results showed no significant differences fGCM concentrations between control birds, suggesting that cause undue stress. individual factors (age, sex, hatching type, display status) associated highlighting need personalized management. Further longitudinal studies needed explore responses captive conditions.

Language: Английский

Citations

0
Validation of enzyme immunoassays for quantifying sex steroid hormones in tropical screech owls (Megascops choliba) DOI Creative Commons
Heriberto Barbosa-Moyano, Rogério Loesch Zacariotti, Cláudio Alvarenga de Oliveira

et al.

General and Comparative Endocrinology, Journal Year: 2023, Volume and Issue: 345, P. 114386 - 114386

Published: Sept. 23, 2023

Language: Английский

Citations

4
Adreno-immunological response to healthcare facility noise during rehabilitation of Tropical Screech Owls DOI Creative Commons
Heriberto Barbosa-Moyano,

Mayra Hespanhol Frediani,

Melissa Peixoto Prosperi

et al.

Global Ecology and Conservation, Journal Year: 2024, Volume and Issue: 50, P. e02845 - e02845

Published: Feb. 16, 2024

Wild animals, particularly those affected by urban expansion, frequently face challenges such as vehicle collisions, and encounters with domestic dogs. Rehabilitation in care facilities, usually conducted ex-situ conditions, poses considerable challenges. In rescue centers, these animals are exposed to a new environment characterized human noise the presence of multiple species confined space. This can potentially trigger stress responses immunosuppression, further compromising their health. study aimed assess endocrine immunological parameters owls (Megascops choliba) under for continuous 49-day period. Twelve adult were audio recordings from center between days 15 35. Fecal samples collected each owl every seven days, well blood eight birds. analyzed glucocorticoid metabolites (GCM), while used measure plasma corticosterone levels, bacterial killing ability (BKA), heterophil-to-lymphocyte ratio (H/L). Our findings revealed that GCM concentration increased during exposure regardless sex, levels remained unchanged. BKA decreased compared before treatment, but H/L did not differ time points. These results show induces alterations allostatic mediators owls, requiring research address other physiological changes individuals may affect recovery maintenance care.

Language: Английский

Citations

1
Multi-Residue Detection of Eight Glucocorticoids by Nano-Au/Fluticasone Propionate Electrochemical Immunosensor DOI Creative Commons
Guozheng Zhao

Molecules, Journal Year: 2023, Volume and Issue: 28(18), P. 6619 - 6619

Published: Sept. 14, 2023

Based on an indirect competitive method, a novel nano-Au/fluticasone propionate electrochemical immunosensor was successfully fabricated by combining the nanoscale effect, superior conductivity of nano-Au, stable Au−S chemical bond as well strong interaction between glucocorticoid and receptor, which used to simultaneously detect eight kinds glucocorticoids. The modified immunosensors’ properties were explored means cyclic voltammetry (CV) method impedance spectroscopy (EIS) measurements. Two factors (glucocorticoid receptor concentration, incubation time) studied in order obtain optimal results. presents attractive performance with wide linear range (between 0.1 1500 ng⋅mL−1) low detection limit 0.057 0.357 ng⋅mL−1), realizing rapid multi-residue large class glucocorticoids (hydrocortisone, triamcinolone) detected actual skincare samples, obtained satisfactory

Language: Английский

Citations

0