Primary metabolomics and transcriptomic techniques were used to explore the regulatory mechanisms that may influence the flavor characteristics of fresh Corylus heterophylla × Corylus avellana

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 30, 2025

To explore the flavor related regulatory mechanisms of fresh Corylus heterophylla × avellana , a joint analysis metabolome and transcriptome were utilized to compare two typical C. varieties with different flavors (‘yuzhui’ ‘pingou21’) in this paper. The results showed that genes including E2.4.1.67-1 E2.4.1.67-2 SUS-1 SUS-2 SUS-4 SUS-5 SUS-7 SUS-8 SUS-9 UGP2-2 identified as responsible for regulating levels stachyose, manninotriose raffinose hazelnuts. CS OGDH deemed involved citric acid cycle, which was central metabolic pathway generated energy through oxidation carbohydrates, fats proteins trpD ALDO PK-1 PK-2 ilvH argE-1 argE-4 argE-5 argD PDAH GLTI regarded biosynthesis various amino acids like tryptophan, valine, alanine, arginine. These determined taste important precursors other flavor-related compounds. LOX2S-2 LOX2S-3 LOX2S-4 LCAT3 viewed regulation lipid biosynthesis, specifically involving 13(S)-HPODE, 9,10,13-trihome 13(S)-HOTrE . findings highlight significance metabolites internal shaping cultivated temperate continents. This study provides theoretical basis breeding excellent food functional hazelnut varieties.

Language: Английский

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Haplotype-resolved T2T genome assemblies and pangenome graph of pear reveal diverse patterns of allele-specific expression and the genomic basis of fruit quality traits

Plant Communications, Journal Year: 2024, Volume and Issue: 5(10), P. 101000 - 101000

Published: June 10, 2024

Hybrid crops often exhibit increased yield and greater resilience, yet the genomic mechanism(s) underlying hybrid vigor or heterosis remain unclear, hindering our ability to predict expression of phenotypic traits in breeding. Here, we generated haplotype-resolved T2T genome assemblies two pear varieties, 'Yuluxiang' (YLX) 'Hongxiangsu' (HXS), which share same maternal parent but differ their paternal parents. We then used these explore genome-scale landscape allele-specific (ASE) create a pangenome graph for pear. ASE was observed close 6000 genes both cultivars. A subset related aspects fruit quality such as sugars, organic acids, cuticular wax were identified, suggesting important contributions heterosis. Specifically, Ma1, gene regulating acidity, is absent haplotypes HXS YLX. built based on seven published genomes. Resequencing data 139 cultivated genotypes (including 97 sequenced here) subsequently aligned graph, revealing numerous structural variant hotspots selective sweeps during diversification. As predicted, Ma1 allele found be varieties with low acid content, this association functionally validated by overexpression calli. Overall, results reveal fruit-quality provide robust reference high-resolution discovery mapping.

Language: Английский

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Genome-wide identification, comparative analysis and functional roles in flavonoid biosynthesis of cytochrome P450 superfamily in pear (Pyrus spp.)

BMC Genomic Data, Journal Year: 2023, Volume and Issue: 24(1)

Published: Oct. 3, 2023

The cytochrome P450 (CYP) superfamily is the largest enzyme metabolism family in plants identified to date, and it involved many biological processes, including secondary metabolite biosynthesis, hormone stress resistance. However, gene has not been well studied pear (Pyrus spp.).Here, comprehensive identification a comparative analysis of members were conducted cultivated wild genomes. In total, 338, 299 419 genes Chinese white pear, European respectively. Based on phylogenetic analyses, divided into ten clans, comprising 48 families. motif structure analyses further supported this classification. expansion was attributed whole-genome single-gene duplication events. Several clusters detected, which have resulted from tandem proximal duplications. Purifying selection major force imposed long-term evolution genes. Gene dosage balance, subfunctionalization neofunctionalization jointly drove retention functional diversification pairs. association between transcriptome expression profiles flavonoid content during fruit development, three candidate as being closely associated with one verified using qRT-PCR its function validated through transient transformation fruit.The study results provide insights functions pear.

Language: Английский

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Exogenous GA3 Enhances Nitrogen Uptake and Metabolism under Low Nitrate Conditions in ‘Duli’ (Pyrus betulifolia Bunge) Seedlings

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7967 - 7967

Published: July 21, 2024

'Duli' (Pyrus betulifolia Bunge) is one of the main rootstocks pear trees in China. Gibberellin (GA) a key plant hormone and roles GA nitrate (NO3-) uptake metabolism plants remain unclear. In this study, we investigated effects exogenous GA3 on N seedlings under NO3- deficiency. The results showed that significantly improves growth On hand, altered root architecture, increased content endogenous hormones (GA3, IAA, ZR), enhanced photosynthesis; other it activities N-metabolizing enzymes accumulation N, expression levels absorption (PbNRT2) genes (PbNR, PbGILE, PbGS, PbGOGAT). However, did not delay degradation chlorophyll. Paclobutrazol had opposite effect growth. Overall, can increase relieve inhibition

Language: Английский

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PyTRM112 is involved in the regulation of GA-mediated fruit size and shape in Pyrus pyrifolia

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 333, P. 113248 - 113248

Published: May 4, 2024

Language: Английский

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Identification and Characterization of the BBX Gene Family in Pomegranate (Punica granatum L.) and Its Potential Role in Anthocyanin Accumulation During Fruit Ripening

Horticulturae, Journal Year: 2025, Volume and Issue: 11(5), P. 507 - 507

Published: May 8, 2025

B-box (BBX) genes, as zinc finger transcription factors (TFs), play essential roles in regulating plant growth and development. In this study, we identified 23 BBX genes the pomegranate (Punica granatum L.) genome. These were classified into five groups based on distribution of conserved domains phylogenetic relationships. Each PgBBX group exhibited similar molecular weights, theoretical isoelectric points (pI), gene structures, motif distributions compared with members Arabidopsis Chinese white pear corresponding groups. Syntenic analysis revealed segmental duplications eight pairs within Additionally, twenty-seven thirty-one orthologous between AtBBX, PbBBX, respectively. Promoter presence types cis-acting elements responding to light, phytohormones, stress, developmental signaling, potential (TFs). GO enrichment confirmed that most function TF involved RNA-seq data indicated PgBBX5 was primarily expressed leaves flowers, increased expression different fruit tissues during ripening. Moreover, showed a high degree sequence similarity anthocyanin-related homologs, including AtBBX24, PhBBX24, FaBBX24, MdCOL4, PyBBX24. During ripening ‘Tunisia’ fruits, positively correlated dynamic changes anthocyanin content key biosynthetic transport genes. Furthermore, subcellular localization suggested encodes nuclear-localized protein. This study provides comprehensive characterization family, offering valuable insights mechanisms underlying accumulation

Language: Английский

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PbrSYP71 regulates ER accumulation by interacting with actin during pollen tube growth in Pyrus

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 20, 2024

Abstract The uneven distribution of endoplasmic reticulum (ER) underlies the rapid polar growth pollen tubes. However, mechanism governing ER remains elusive. In this study, we have identified a tube-specific syntaxin protein, PbrSYP71. Our findings reveal that both overexpression and knocking down PbrSYP71 inhibited tube growth. Subcellular localization analysis demonstrates anchors to via its transmembrane structure. Overexpression leads clustered in tube, while abolishes distribution. Remarkably, transient Δ ABD , lacking actin binding domain (ABD) PbrSYP71, has no impact on or Further investigation indicates is positioned F-actin direct interaction with F-actin. assists moving towards apex displaying autonomous mobility. study elucidates maintains by tethering F-actin, facilitating movement for pear elongation. These insights shed light mechanisms polarized cell

Language: Английский

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Comparative analysis of POD genes and their expression under multiple hormones in Pyrus bretschenedri

BMC Genomic Data, Journal Year: 2024, Volume and Issue: 25(1)

Published: May 6, 2024

Abstract Background Class III peroxidase (POD) enzymes play vital roles in plant development, hormone signaling, and stress responses. Despite extensive research on POD families various species, the knowledge regarding family Chinese pear ( Pyrus bretschenedri ) is notably limited. Results We systematically characterized 113 genes, designated as PbPOD1 to PbPOD113 based their chromosomal locations. Phylogenetic analysis categorized these genes into seven distinct subfamilies (I VII). The segmental duplication events were identified a prevalent mechanism driving expansion of gene family. Microsynteny analysis, involving comparisons with , Fragaria vesca Prunus avium mume persica highlighted conservation duplicated regions persistence through purifying selection during evolutionary process. expression patterns PbPOD performed across organs diverse fruit development stages using transcriptomic data. Furthermore, we stress-related cis -acting elements within promoters underscoring involvement hormonal environmental Notably, qRT-PCR analyses revealed distinctive response melatonin (MEL), salicylic acid (SA), abscisic (ABA), methyl jasmonate (MeJA), reflecting responsiveness abiotic role growth development. Conclusions In this study, investigated potential functions dynamics positioning them promising candidates for further valuable indicators enhancing quality molecular breeding strategies.

Language: Английский

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The MYC transcription factor PbrMYC8 negatively regulates PbrMSL5 expression to promote pollen germination in Pyrus

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134640 - 134640

Published: Aug. 12, 2024

Language: Английский

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GH3 Gene Family Identification in Chinese White Pear (Pyrus bretschneideri) and the Functional Analysis of PbrGH3.5 in Fe Deficiency Responses in Tomato

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12980 - 12980

Published: Dec. 3, 2024

Iron (Fe) deficiency poses a major threat to pear (

Language: Английский

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