Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro DOI Creative Commons
David J. Dowling, Simone Schüller, Soumik Barman

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 27, 2023

Abstract Vaccination reduces morbidity and mortality due to infections, but efficacy may be limited distinct immunogenicity at the extremes of age. This raises possibility employing adjuvants enhance protection. Early IFNγ production is a hallmark effective vaccine in adults serving as biomarker that predict adjuvanticity. We utilized mass cytometry (CyTOF) dissect source adjuvant-induced cytokine human blood mononuclear cells (BMCs) from newborns (~39-week-gestation), (~18-63 years old) elders (>65 age) after stimulation with pattern recognition receptors agonist (PRRa) adjuvants. Dimensionality reduction analysis CyTOF data mapped BMC compartment, elucidated age-specific immune responses profiled PRR-mediated activation monocytes DCs upon adjuvant stimulation. Furthermore, we demonstrated PRRa mediated innate induction NK key TLR7/8 (TLR7/8a) specific responses. Hierarchical clustering revealed age TLR7/8a-specific accumulation producing γδ T cells. Our study demonstrates application cutting-edge computational approaches characterize across immunologically groups inform identification bespoke adjuvantation systems tailored immunity vulnerable populations.

Language: Английский

Memory Cells in Infection and Autoimmunity: Mechanisms, Functions, and Therapeutic Implications DOI Creative Commons
Shilpi Giri, Lalit Batra

Vaccines, Journal Year: 2025, Volume and Issue: 13(2), P. 205 - 205

Published: Feb. 19, 2025

Memory cells are central to the adaptive immune system's ability remember and respond effectively previously encountered pathogens. While memory provide robust protection against infections, they can also contribute autoimmunity when regulation fails. Here, we review roles of T B in infection autoimmunity, focusing on their differentiation, activation, effector functions, underlying regulatory mechanisms. We elaborate precise mechanisms by which autoimmune diseases, highlighting insights from current research how pathogenic responses formed sustained autoimmunity. Finally, explore potential therapeutic strategies aimed at modulating prevent or treat disorders, including cell-depleting therapies (e.g., Rituximab), cell-targeting agents Abatacept), cytokine inhibitors IL-17 IL-23 blockers) that currently used diseases such as rheumatoid arthritis, multiple sclerosis, psoriasis.

Language: Английский

Citations

0
Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro DOI Creative Commons
Simone Schüller, Soumik Barman, Raúl Méndez-Giráldez

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 8, 2024

Vaccination reduces morbidity and mortality due to infections, but efficacy may be limited distinct immunogenicity at the extremes of age. This raises possibility employing adjuvants enhance protection. Early IFNγ production is a hallmark effective vaccine in adults serving as biomarker that predict adjuvanticity. We utilized mass cytometry (CyTOF) dissect source adjuvant-induced cytokine human blood mononuclear cells (BMCs) from newborns (~39-week-gestation), (~18-63 years old) elders (>65 age) after stimulation with pattern recognition receptors agonist (PRRa) adjuvants. Dimensionality reduction analysis CyTOF data mapped BMC compartment, elucidated age-specific immune responses profiled PRR-mediated activation monocytes DCs upon adjuvant stimulation. Furthermore, we demonstrated PRRa mediated innate induction NK key TLR7/8 (TLR7/8a) specific responses. Hierarchical clustering revealed age TLR7/8a-specific accumulation producing γδ T cells. Our study demonstrates application cutting-edge computational approaches characterize across immunologically groups inform identification bespoke adjuvantation systems tailored immunity vulnerable populations.

Language: Английский

Citations

3
Lung influenza virus specific memory CD4 T cell location and optimal cytokine production are dependent on interactions with lung antigen-presenting cells DOI Creative Commons
Kerrie E Hargrave, Julie C. Worrell, Chiara Pirillo

et al.

Mucosal Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: June 1, 2024

Language: Английский

Citations

1
Adapting for success: T cell features at tissue sites DOI Creative Commons
Philip P. Ahern, Emily Gwyer Findlay

Discovery Immunology, Journal Year: 2024, Volume and Issue: 3(1)

Published: Jan. 1, 2024

Language: Английский

Citations

0
Lung structural cell dynamics are altered by influenza virus infection experience leading to rapid immune protection following viral re-challenge DOI Creative Commons
Julie C. Worrell, Kerrie E Hargrave, George Finney

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Lung structural cells, including epithelial cells and fibroblasts, form barriers against pathogens trigger immune responses following infections such as influenza A virus. This response leads to the recruitment of innate adaptive required for viral clearance. Some these recruited remain within lung infection contribute enhanced control subsequent infections. There is growing evidence that can also display long-term changes or insults. Here we investigate mouse endothelial virus find all three cell types maintain an imprint infection, particularly in genes associated with communication T cells. from IAV-infected mice functional by more rapidly controlling than naïve animals. rapid anti-viral increased expression molecules communicate demonstrates sustained functions infection. These data suggest could be effective targets vaccines boost durable protective immunity. Graphical Highlights blood inflammatory (IAV) at least 40 days post-infection. In vivo re-infection a spatially restricted compared primary are not early after Ex IAV animals absence

Language: Английский

Citations

0
Immune profiling of age and adjuvant-specific activation of human blood mononuclear cells in vitro DOI Creative Commons
David J. Dowling, Simone Schüller, Soumik Barman

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 27, 2023

Abstract Vaccination reduces morbidity and mortality due to infections, but efficacy may be limited distinct immunogenicity at the extremes of age. This raises possibility employing adjuvants enhance protection. Early IFNγ production is a hallmark effective vaccine in adults serving as biomarker that predict adjuvanticity. We utilized mass cytometry (CyTOF) dissect source adjuvant-induced cytokine human blood mononuclear cells (BMCs) from newborns (~39-week-gestation), (~18-63 years old) elders (>65 age) after stimulation with pattern recognition receptors agonist (PRRa) adjuvants. Dimensionality reduction analysis CyTOF data mapped BMC compartment, elucidated age-specific immune responses profiled PRR-mediated activation monocytes DCs upon adjuvant stimulation. Furthermore, we demonstrated PRRa mediated innate induction NK key TLR7/8 (TLR7/8a) specific responses. Hierarchical clustering revealed age TLR7/8a-specific accumulation producing γδ T cells. Our study demonstrates application cutting-edge computational approaches characterize across immunologically groups inform identification bespoke adjuvantation systems tailored immunity vulnerable populations.

Language: Английский

Citations

1