Divergence in Regulatory Regions and Gene Duplications May Underlie Chronobiological Adaptation in Desert Tortoises DOI

Norman. Mellor,

Timothy H. Webster, Hazel Byrne

et al.

Molecular Ecology, Journal Year: 2024, Volume and Issue: 34(2)

Published: Dec. 3, 2024

Many cellular processes and organismal behaviours are time-dependent, asynchrony of these phenomena can facilitate speciation through reinforcement mechanisms. The Mojave Sonoran desert tortoises (Gopherus agassizii G. morafkai respectively) reside in adjoining deserts with distinct seasonal rainfall patterns they exhibit asynchronous winter brumation reproductive behaviours. We used whole genome sequencing 21 individuals from the two tortoise species an outgroup to understand genes potentially underlying characteristics. Genes within most diverged 1% (F

Language: Английский

Haplotype-resolved chromosomal-level assembly of wasabi (Eutrema japonicum) genome DOI Creative Commons
Hiroyuki Tanaka,

Tatsuki Hori,

Shohei Yamamoto

et al.

Scientific Data, Journal Year: 2023, Volume and Issue: 10(1)

Published: July 11, 2023

Abstract In Japan, wasabi ( Eutrema japonicum ) is an important traditional condiment, and recognized as endemic species. the present study, we generated a chromosome-level haplotype-resolved reference genome for E. using PacBio CLR (continuous long reads), Illumina, Hi-C sequencing data. The consists of 28 chromosomes that contain 1,512.1 Mb sequence data, with scaffold N50 length 55.67 Mb. We also reported subgenome haplotype assignment by read-mapping phylogenic analysis. Three validation methods (Benchmarking Universal Single-Copy Orthologs, Merqury, Inspector) indicated our obtained sequences were high-quality high-completeness assembly. Comparison assemblies from previously published genomes showed was higher quality. Therefore, will serve valuable genetic resource both chemical ecology evolution research genera Brassicaceae, well breeding.

Language: Английский

Citations

5

Increased photosystem II translation efficiency as an important photoprotective mechanism in an Arabidopsis thaliana ecotype (Tibet-0) adapted to high light environments DOI Creative Commons
Man Zhang, Jing Zhao, Wanying Li

et al.

Environmental and Experimental Botany, Journal Year: 2020, Volume and Issue: 183, P. 104350 - 104350

Published: Dec. 16, 2020

The Qinghai-Tibet Plateau (QTP) is the highest and largest young plateau in world, with a wide variety of extreme environments. Due to low density air high transparency atmosphere, QTP has abundant sunshine light intensity. It important for plants evolve mechanism photoprotection adapt high-light conditions. Arabidopsis thaliana an model organism plant science molecular biology. Tibet-0 was first ecotype collected QTP, at >4000 m above sea level. Here, we systematically analyzed photosynthetic responses light. Our results show that more efficient PSII activity under continuous conditions (1000 μmol photons m−2 s−1) compared Columbia (Col-0). Chlorophyll fluorescence analysis showed exhibited enhanced quantum yield (ФPSII), photochemical quenching (qP), electron transport rate (ETR) However, anthocyanin accumulation lower Tibet-0. Blue Native-PAGE immunoblot analyses abundances complexes subunits were significantly higher vs. Col-0 standard Notably, associations core subunit mRNAs psbA, psbB, psbC psbD chloroplast ribosomal protein Tibet-0, based on ribosome-nascent chain complex (RNC)-quantitative PCR. Moreover, expression amounts protein-related genes activated after treatment. These suggest capability arises via constitutively translation efficiency proteins due expression. This study thus provides insight into underlying adaptation altitudes.

Language: Английский

Citations

12

Genomics of ecological adaptation in Canary Island Descurainia (Brassicaceae) and comparisons with other Brassicaceae DOI Creative Commons
Amy C. Jackson, Mark A. Carine, Mark A. Chapman

et al.

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(8)

Published: Aug. 1, 2024

Abstract Oceanic archipelagos provide striking examples of lineages that have radiated over pronounced ecological gradients. Accompanying this diversification, evolved adaptations allowing survival in extreme environments. Here, we investigate the genomic basis adaptation Canary Island Descurainia (Brassicaceae), an island relative Arabidopsis . The seven endemic species diversified situ along elevational and gradient, from low‐elevation scrub to high‐elevation sub‐alpine desert. We first generated a reference genome for millefolia, phylogenetic analysis which placed it as sister D. sophioides Ninety‐six gene families were found be specific millefolia further 1087 1469 expanded or contracted size, respectively, branch. then employed re‐sequencing sample 14 genomes across outgroup. Phylogenomic analyses consistent with previous reconstructions resolving low‐ clades. Using branch‐site dN/dS method, detected positive selection 275 genes on branch separating these positively selected (PSGs) significantly enriched functions related reproduction stress tolerance. Comparing PSGs those elevation and/or latitude other Brassicaceae, little evidence widespread convergence reuse, except two examples, one was significant overlap between Draba nivalis, restricted high latitudes. study suggests transition environments such mountains Islands involves tolerance but repeated evolution different into similar habitats is limited, indicating substantially molecular trajectories adaptation.

Language: Английский

Citations

1

Adaptation of High-Altitude Plants to Harsh Environments: Application of Phenotypic-Variation-Related Methods and Multi-Omics Techniques DOI Open Access

Kai‐Lu Zhang,

Ya-Nan Leng,

Ruirui Hao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12666 - 12666

Published: Nov. 26, 2024

High-altitude plants face extreme environments such as low temperature, oxygen, nutrient levels, and strong ultraviolet radiation, causing them to adopt complex adaptation mechanisms. Phenotypic variation is the core manifestation of ecological evolution. Many have developed a series adaptive strategies through long-term natural selection evolution, enabling survive reproduce under harsh conditions. This article reviews techniques methods used in recent years study evolution high-altitude plants, including transplantation techniques, genomics, transcriptomics, proteomics, metabolomics their applications plant Transplantation technology focuses on phenotypic variation, which refers variations morphological, physiological, biochemical characteristics, exploring key roles utilization, photosynthesis optimization, stress-resistance protection. Multiple omics technologies, metabolomics, revealed genes, regulatory pathways, metabolic networks associated with at genetic molecular levels. At same time, limitations deficiencies current technologies were discussed. In addition, we propose future improvements existing advocate for integration different multiple levels mechanisms environments, thus providing insights research this field.

Language: Английский

Citations

1

Divergence in Regulatory Regions and Gene Duplications May Underlie Chronobiological Adaptation in Desert Tortoises DOI

Norman. Mellor,

Timothy H. Webster, Hazel Byrne

et al.

Molecular Ecology, Journal Year: 2024, Volume and Issue: 34(2)

Published: Dec. 3, 2024

Many cellular processes and organismal behaviours are time-dependent, asynchrony of these phenomena can facilitate speciation through reinforcement mechanisms. The Mojave Sonoran desert tortoises (Gopherus agassizii G. morafkai respectively) reside in adjoining deserts with distinct seasonal rainfall patterns they exhibit asynchronous winter brumation reproductive behaviours. We used whole genome sequencing 21 individuals from the two tortoise species an outgroup to understand genes potentially underlying characteristics. Genes within most diverged 1% (F

Language: Английский

Citations

1