Culture‐Independent Species‐Level Taxonomic and Functional Characterisation of Bacteroides, the Core Bacterial Genus Within Reptile Guts

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

The genus Bacteroides is a widespread and abundant bacterial taxon associated with gut microbiotas. Species within fill many niches, including as mutualists, commensals pathogens for their hosts. Within reptiles, dominant, 'core' bacterium that sometimes exhibits increased abundance in times of food scarcity, such during hibernation. Here, we take two-pronged approach to better characterise populations reptile guts. Firstly, leverage published 16S rRNA gene sequence datasets determine the species-level distributions members Secondly, mine publicly available metagenomes extract data from birds, amphibians mammals, compare functional potential different host taxa. analyses revealed B. acidifaciens most common species guts, orders reptiles differ which they harbour. taxonomy recovered metagenomic assembly did not between or substantially across mammals. Metagenome-assembled genomes were marginally more related when hosts closely related, particular harbouring markedly unique MAGs compared other Our findings indicate harbour similar profiles broad comparisons, but some differences groups, appears perform largely roles vertebrate guts regardless relatedness.

Language: Английский

Increased host diversity limits bacterial generalism but may promote microbe-microbe interactions

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 26, 2024

Abstract Host-associated bacteria vary in their host breadth, which can impact ecological interactions. By colonizing diverse hosts, generalists have disproportionate impacts. For bacteria, generalism may advantageous, particularly when the availability of specific hosts is variable. It unclear how much ability to evolve generalism, by inhabiting constrained host-associated bacteria. We hypothesized that constraints on bacterial will differ depending species. To test this, we assessed patterns diversity and specialization cloacal microbiomes reptile communities from temperate zone tropics, where abundance species varies substantially. Within these communities, generalist taxa tended be Proteobacteria, whereas specialists Firmicutes. found were less prevalent highest keeping with Proteobacteria communities. Generalist became relatively widespread across only two most therefore conclude constraint not driven absolute incompatibility some species, but rather competition adapted specialist lineages. In high-diversity successful generalists, typically disproportionately likely co-occur one another evolutionarily disparate within community. Our data indicate lineages adapt evolutionary pressures high either specializing or forming cohorts co-occurring

Language: Английский

In Situ Localization of Staphylococcus shinii and Staphylococcus succinus in Infected Rhipicephalus microplus Ticks: Implications for Biocontrol Strategies

Pathogens, Journal Year: 2024, Volume and Issue: 13(12), P. 1025 - 1025

Published: Nov. 21, 2024

Rhipicephalus microplus is a blood-sucking parasite that causes heavy infestations on cattle and vector for severe tick-borne diseases, such as anaplasmosis babesiosis, poses significant threat to the industry. Cattle ticks show increasing acaricide resistance, which creates an additional problem concerning inefficient chemical control of tick populations in cattle-grazing areas, necessitating exploration alternative biocontrol methods. Our study aimed demonstrate acaropathogenic efficacy two bacterial species during experimental infections R. microplus. data confirmed S. shinii succinus exhibited properties against microplus, demonstrated by tracking fluorescent-labeled bacteria within engorged-tick body. experiments revealed both could infect hemolymph, salivary glands, vestibular vagina tick, inducing histological changes affected organs may impair feeding well reproductive capabilities. Gené’s organ infection was detected only succinus. findings offer valuable insights developing strategies manage effectively.

Language: Английский