A biobank resource for placental malaria and placental health research DOI Creative Commons

Melvin Mbalitsi,

Mercy Singoei, Samuel Chenge

et al.

Open Research Europe, Journal Year: 2024, Volume and Issue: 4, P. 273 - 273

Published: Dec. 24, 2024

Background Malaria during pregnancy is a serious threat to maternal and child health in malaria-endemic regions. often leads placental malaria, which has severely detrimental effects on fetal outcomes, including growth restriction, preterm delivery, stillbirth. However, the mechanisms by malaria causes adverse outcomes remain poorly understood. Crucially, there are no tools for detecting and/or intervening pregnancy. This mainly because placenta inaccessible research Nonetheless, its accessibility after delivery offers unique opportunities studying diseases. To support such research, collecting biobanking naturally-infected samples geographical context diseases of interest occur crucial. Methods Here, we describe establishment biobank obtained from region Kenya, aims enhance capacity this important but under-studied challenge. Histology, PCR, isothermal identical multirepeat sequence PCR were used determine status samples. Results Our characterization indicates that affects most pregnancies region, despite mothers being asymptomatic having received anti-malaria chemoprophylaxis. Of techniques detect detected highest number malaria-positive (54.2%), compared with histology (37.3%), tissue (17%). results various did not overlap fully. Conclusion observations highlight need rigorously characterize regions, using multiple complementary techniques, even cases undetectable It may also be beneficial obtain non-malaria-endemic regions use as malaria-naïve controls.

Language: Английский

Endocytosis at the maternal-fetal interface: balancing nutrient transport and pathogen defense DOI Creative Commons
Mingming Fan, Hongyu Wu, Amanda N. Sferruzzi‐Perri

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 18, 2024

Endocytosis represents a category of regulated active transport mechanisms. These encompass clathrin-dependent and -independent mechanisms, as well fluid phase micropinocytosis macropinocytosis, each demonstrating varying degrees specificity capacity. Collectively, these mechanisms facilitate the internalization cargo into cellular vesicles. Pregnancy is one such physiological state during which endocytosis may play critical roles. A successful pregnancy necessitates ongoing communication between maternal fetal cells at maternal-fetal interface to ensure immunologic tolerance for semi-allogenic fetus whilst providing adequate protection against infection from pathogens, viruses bacteria. It also requires nutrients across interface, but restriction potentially harmful chemicals drugs allow development. In this context, trogocytosis, specific form endocytosis, plays crucial role in immunological prevention. thought significant nutrient toxin handling though its remain less understood. comprehensive understanding not only enhances our knowledge interactions essential identifying pathogenesis pathologies new avenues therapeutic intervention.

Language: Английский

Citations

3
A biobank resource for placental malaria and placental health research DOI Creative Commons

Melvin Mbalitsi,

Mercy Singoei, Samuel Chenge

et al.

Open Research Europe, Journal Year: 2024, Volume and Issue: 4, P. 273 - 273

Published: Dec. 24, 2024

Background Malaria during pregnancy is a serious threat to maternal and child health in malaria-endemic regions. often leads placental malaria, which has severely detrimental effects on fetal outcomes, including growth restriction, preterm delivery, stillbirth. However, the mechanisms by malaria causes adverse outcomes remain poorly understood. Crucially, there are no tools for detecting and/or intervening pregnancy. This mainly because placenta inaccessible research Nonetheless, its accessibility after delivery offers unique opportunities studying diseases. To support such research, collecting biobanking naturally-infected samples geographical context diseases of interest occur crucial. Methods Here, we describe establishment biobank obtained from region Kenya, aims enhance capacity this important but under-studied challenge. Histology, PCR, isothermal identical multirepeat sequence PCR were used determine status samples. Results Our characterization indicates that affects most pregnancies region, despite mothers being asymptomatic having received anti-malaria chemoprophylaxis. Of techniques detect detected highest number malaria-positive (54.2%), compared with histology (37.3%), tissue (17%). results various did not overlap fully. Conclusion observations highlight need rigorously characterize regions, using multiple complementary techniques, even cases undetectable It may also be beneficial obtain non-malaria-endemic regions use as malaria-naïve controls.

Language: Английский

Citations

0