Contributions of F‐specific subunits to the F plasmid‐encoded type IV secretion system and F pilus DOI Open Access
Kouhei Kishida, Rachel E. Bosserman, Laith Harb

et al.

Molecular Microbiology, Journal Year: 2022, Volume and Issue: 117(5), P. 1275 - 1290

Published: April 18, 2022

F plasmids circulate widely among the Enterobacteriaceae through encoded type IV secretion systems (T4SSF s). Assembly of T4SSF s and associated pili requires 10 VirB/VirD4-like Tra subunits eight or more F-specific subunits. Recently, we presented evidence using in situ cryoelectron tomography (cryoET) that undergo structural transitions when activated for pilus production, assembled are deposited onto alternative basal platforms at cell surface. Here, deleted conserved genes from MOBF12C plasmid pED208 quantitated effects on transfer, production by fluorescence microscopy, elaboration structures cryoET. Mutant phenotypes supported assignment into three functional Classes: (i) TraF, TraH, TraW required all -associated activities, (ii) TraU, TraN, TrbC nonessential but contribute significantly to distinct functions, (iii) TrbB is essential not transfer. Equivalent mutations a phylogenetically distantly related MOB12A conferred similar generally these Class assignments. We present new structure-driven model which steps assembly activation regulate DNA transfer dynamics deposition platforms.

Language: Английский

Verrucomicrobia of the Family Chthoniobacteraceae Participate in Xylan Degradation in Boreal Peat Soils DOI Creative Commons
Andrey L. Rakitin, Irina S. Kulichevskaya, Alexey V. Beletsky

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(11), P. 2271 - 2271

Published: Nov. 8, 2024

The phylum

Language: Английский

Citations

5
The role and function mechanism of tapP in modulating the virulence of Aeromonas hydrophila DOI
Rui Long,

Jinming Wei,

Caijiang Xiong

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 591, P. 741104 - 741104

Published: May 18, 2024

Language: Английский

Citations

4
A Phage‐Based Approach to Identify Antivirulence Inhibitors of Bacterial Type IV Pili DOI Creative Commons

Tori M. Shimozono,

Nancy J. Vogelaar,

Megan T. O’Hara

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(1)

Published: Jan. 1, 2025

The increasing threat of antibiotic resistance underscores the urgent need for innovative strategies to combat infectious diseases, including development antivirulants. Microbial pathogens rely on their virulence factors initiate and sustain infections. Antivirulants are small molecules designed target factors, thereby attenuating microbes. bacterial type IV pilus (T4P), an extracellular protein filament that depends T4P machinery (T4PM) its biogenesis, dynamics function, is a key factor in many significant pathogens. While T4PM presents promising antivirulence target, systematic identification inhibitors multiple constituents remains considerable challenge. Here we report novel high-throughput screening (HTS) approach discovering inhibitors. It uses Pseudomonas aeruginosa, high-priority pathogen, combination with T4P-targeting phage, φKMV. Screening library 2168 compounds using optimised protocol led tuspetinib, based deterrence lysis P. aeruginosa by Our findings show tuspetinib also inhibits two additional phages, while having no effect phage recognises lipopolysaccharides as receptor. Additionally, impedes T4P-mediated motility Acinetobacter species without impacting growth or flagellar motility. This bacterium-phage pairing applicable broad range required infection, paving ways advanced chemotherapeutics against antibiotic-resistant

Language: Английский

Citations

0
The role and function mechanism of pilV in modulating the pathogenicity of Aeromonas hydrophila DOI

Zhengshi Zhang,

Jiayan Ran,

Shengfu Zhao

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742258 - 742258

Published: Feb. 1, 2025

Language: Английский

Citations

0
Synergistic action of mucoactive drugs and phages against Pseudomonas aeruginosa and Klebsiella pneumoniae DOI Creative Commons

Bingrui Sui,

Xiaoyu Li, Na Li

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT N -acetylcysteine (NAC) and ambroxol hydrochloride (AMB) are commonly prescribed alongside antibiotics to alleviate sputum retention in lower respiratory tract infections, which often caused by bacterial pathogens. With the rising threat of antibiotic resistance, phage therapy has emerged as a promising alternative alongside. However, no studies have explored potential interactions between phages these mucoactive agents despite their frequent concurrent use during therapy. Therefore, investigating synergy its subsequent impact on infection dynamics could enhance clinical strategies for treating infections with phages. Our study utilized Pseudomonas aeruginosa strain ZS-PA-35 Klebsiella pneumoniae Kp36, respective phages, investigate presence NAC or AMB. findings indicate that, under specific conditions, can function adjuvants lytic enhancing susceptibility facilitating proliferation. revealed that synergistic strongly influenced physiological characteristics surrounding microenvironments, physiology host tissues, varying outcomes phage-host were observed among different across distinct media. Taken together, our results emphasize complexity AMB, underscoring need caution when using combination treatments. IMPORTANCE used medical treatment patients acute chronic bronchitis. Often, choice AMB is empirically determined physicians. combining remains unclear. To address this gap, comprehensive understanding interplay crucial determine any effects. This aims elucidate how influence targeting receptors, thereby affecting antibacterial activity against . suggest certain provides an adjuvant effect rendering cells more susceptible infection. These contribute advancing therapy, offering insights optimizing efficacy.

Language: Английский

Citations

0
Stimulation of theCaulobacter crescentussurface sensing pathway by deletion of a specialized minor pilin-like gene DOI Creative Commons
Farah Obeid Charrouf, Gregory B. Whitfield, Courtney K. Ellison

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Abstract Bacteria colonize surfaces through complex mechanisms of surface sensing. Pili are dynamic bacterial appendages that play an important role in this process. In Caulobacter crescentus , tension on retracting, surface-bound pili triggers the rapid synthesis adhesive holdfast, which permanently attaches cells to surfaces. However, detailed pilus-mediated sensing unclear. study, we used a genetic screen isolate mutants with altered pilus activity identify genes may be involved surface-sensing. This identified cpaL whose deletion led reduced piliation levels, and surprisingly, threefold increase adhesion due increased holdfast production. To understand finding, compared wild-type mutant under conditions block retraction. While treatment production by triggering surface-sensing pathway, no was observed mutant, suggesting mutation maximally stimulates Furthermore, when grown medium blocks exhibited decreased attachment production, consistent for CpaL pilus-dependent C. . better function CpaL, analyzed its predicted structure, suggested is minor pilin fused mechanosensitive von Willebrand factor type A (vWA) domain could accommodated at tip. These results collectively position as strong candidate mechanosensory element Importance Surface crucial mechanism allows bacteria change their behaviors adapt life surface. recognition initial step toward colonization biofilm formation. tight adherence (Tad) key adaptation. proteins influence process remain unknown. Here, demonstrate potential tip protein, major Tad plays regulation upon contact. By identifying player recognition, our work offers valuable insights into adhesion.

Language: Английский

Citations

0
The role of PilU in the surface behaviors of Pseudomonas aeruginosa DOI Creative Commons
Jingchao Zhang, Yan Luo, Yiwu Zong

et al.

mLife, Journal Year: 2025, Volume and Issue: 4(1), P. 83 - 95

Published: Feb. 1, 2025

Abstract In Pseudomonas aeruginosa , the dynamic activity of type IV pilus (TFP) is essential for various bacterial behaviors. While PilU considered a homolog TFP disassembling motor PilT, its specific roles remain unclear. Using visualization and single‐cell tracking techniques, we characterized dynamics surface behaviors in wild‐type Δ pilU mutants. We found that cells displayed increased numbers but reduced cell movement delayed microcolony formation. Interestingly, beyond affecting twitching motility, formed thick multilayered colony edge on semi‐solid surfaces, slowing expansion. Cell–cell collision responses changed from touch‐turn dominance wild to touch‐upright morphology These findings expand our understanding PilU's physiological provide potential targets developing strategies control P. biofilm formation virulence.

Language: Английский

Citations

0
Phage-host interaction in Pseudomonas aeruginosa clinical isolates with functional and altered quorum sensing systems DOI Creative Commons

Dandan Li,

Na Li, Yu Chen

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

ABSTRACT Quorum sensing (QS) plays a crucial role in regulating key traits, including the upregulation of phage receptors, which leads to heightened susceptibility Pseudomonas aeruginosa . As result, higher cell densities typically increase risk invasions. This has led speculation that bacteria may have evolved strategies counterbalance this increased susceptibility. Additionally, non-synonymous mutations LasR, master regulator QS, are common among cystic fibrosis patients, but impact these on interactions remains poorly understood. Here, we systematically investigated QS shaping using bacterial strains with functional or altered systems. In QS-functional strain ZS-PA-35, disruption Las system reduces type IV pili-dependent phipa2, delaying lysis during early logarithmic growth phase. At high densities, Las-induced dormancy further inhibits proliferation despite enhanced adsorption. Notably, nutrient supplementation fully restores system. contrast, QS-deficient ZS-PA-05, carrying LasR mutation, fails regulate phage-host via QS. Moreover, our findings reveal within mixed microbial populations, cells benefit from presence closely related kin, collectively reduce prey density and limit interaction frequencies under nutrient-rich conditions. These results underscore flexibility QS-regulated defense strategies, highlighting their critical optimizing resilience against predation, particularly heterogeneous communities most vulnerable phages. IMPORTANCE Bacteria developed various combat infection, posing challenges therapy. study, demonstrate quorum systems adapt different survival tactics for prolonged coexistence phages, contingent upon population dynamics. The dynamics infection highlight influence intrinsic heterogeneity mediated by emergence outcomes. variants arise as result coevolutionary processes mechanisms mutational non-mutational strategies. insights enhance comprehension how shield themselves attacks complexity such approaches successful therapeutic interventions.

Language: Английский

Citations

0
Contribution of the type IV pili secretin tapQ to motility, growth, adhesion, stress tolerance and virulence of Aeromonas hydrophila DOI

Zebin Liao,

Rui Long,

Wan’e Ding

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142203 - 142203

Published: March 1, 2025

Language: Английский

Citations

0
Type IV Pili in Thermophilic Bacteria: Mechanisms and Ecological Implications DOI Creative Commons

Naoki A. Uemura,

Daisuke Nakane

Biomolecules, Journal Year: 2025, Volume and Issue: 15(4), P. 459 - 459

Published: March 21, 2025

Type IV pili (T4P) machinery is critical for bacterial surface motility, protein secretion, and DNA uptake. This review highlights the ecological significance of T4P-dependent motility in Thermus thermophilus, a thermophilic bacterium isolated from hot springs. Unlike swimming T4P enables bacteria to move over two-dimensional surfaces through repeated cycles extension retraction pilus filaments. Notably, T. thermophilus exhibits upstream-directed migration under shear stress, known as rheotaxis, which appears represent an adaptive strategy unique thriving rapid water flows. Furthermore, contributes capture phages, indicating their multifunctionality natural environments. Understanding dynamics provides insights into survival evolution extreme habitats.

Language: Английский

Citations

0