Tolerance and Resistance of Pseudomonas aeruginosa Biofilms to Antimicrobial Agents—How P. aeruginosa Can Escape Antibiotics

Frontiers in Microbiology, Journal Year: 2019, Volume and Issue: 10

Published: May 3, 2019

The ESKAPE pathogen Pseudomonas aeruginosa is recognized as an important cause of chronic infections due to its ability form biofilms where the bacteria are present in aggregates encased a self-produced extracellular matrix and difficult or impossible eradicate with antibiotic treatment. P. causing lungs patients cystic fibrosis obstructive lung disease, well urinary tract permanent bladder catheter, ventilator-associated pneumonia intubated patients, also wounds. Antibiotic treatment cannot these biofilm their intrinsic tolerance development mutational resistance. antibiotics multifactorial involving physical, physiological genetic determinants, whereas resistance caused by mutations driven repeated exposure high levels antibiotics. In this review both antimicrobial discussed. Possible therapeutic approaches based on understanding mechanisms involved resistances addressed.

Language: Английский

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New Perspectives in Biofilm Eradication

ACS Infectious Diseases, Journal Year: 2017, Volume and Issue: 4(2), P. 93 - 106

Published: Dec. 27, 2017

Microbial biofilms, which are elaborate and highly resistant microbial aggregates formed on surfaces or medical devices, cause two-thirds of infections constitute a serious threat to public health. Immunocompromised patients, individuals who require implanted artificial limbs, organ transplants, external life support those with major injuries burns, particularly prone become infected. Antibiotics, the mainstay treatments bacterial infections, have often proven ineffective in fight against microbes when growing as date, no antibiotic has been developed for use biofilm infections. Antibiotic resistance is rising, but biofilm-mediated multidrug transcends this being adaptive broad spectrum dependent growth state organisms. Therefore, treatment biofilms requires drug developers start thinking outside constricted "antibiotics" box find alternative ways target Here, we highlight recent approaches combating focusing eradication preformed including electrochemical methods, promising antibiofilm compounds progress delivery strategies enhance bioavailability potency agents.

Language: Английский

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Evolutionary pathways to antibiotic resistance are dependent upon environmental structure and bacterial lifestyle

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Sept. 13, 2019

Bacterial populations vary in their stress tolerance and population structure depending upon whether growth occurs well-mixed or structured environments. We hypothesized that evolution biofilms would generate greater genetic diversity than environments lead to different pathways of antibiotic resistance. used experimental whole genome sequencing test how the biofilm lifestyle influenced rate, mechanisms, pleiotropic effects resistance ciprofloxacin Acinetobacter baumannii populations. Both evolutionary dynamics identities mutations differed between lifestyle. Planktonic experienced selective sweeps including primary topoisomerase drug targets, whereas biofilm-adapted acquired regulators efflux pumps. An overall trade-off fitness level emerged, wherein clones were less resistant planktonic but more fit absence drug. However, developed collateral sensitivity cephalosporins, demonstrating clinical relevance on

Language: Английский

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Inhibiting bacterial cooperation is an evolutionarily robust anti-biofilm strategy

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Jan. 9, 2020

Abstract Bacteria commonly form dense biofilms encased in extracellular polymeric substances (EPS). Biofilms are often extremely tolerant to antimicrobials but their reliance on shared EPS may also be a weakness as social evolution theory predicts that inhibiting traits can select against resistance. Here we show of Salmonella is cooperative trait whose benefit among cells, and inhibition reduces both cell attachment antimicrobial tolerance. We then compare an inhibitor conventional evolutionary experiment. While resistance rapidly evolves, see no inhibition. further resistant strain outcompeted by susceptible under treatment, explaining why does not evolve. Our work suggests targeting viable solution the problem

Language: Английский

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Biofilm formation and its implications on the properties and fate of microplastics in aquatic environments: A review

Journal of Hazardous Materials Advances, Journal Year: 2022, Volume and Issue: 6, P. 100077 - 100077

Published: April 21, 2022

Particulate plastic fragments (micro and nano-plastics) in aquatic environments provide abundant solid substrates, which serve as an important habitat for a variety of microorganisms. Surfaces microplastics (MPs) exhibit hydrophobicity that facilitate the adsorption dissolved organic carbon (DOC) environment. Furthermore, MPs act substrata, well source including readily bioavailable DOC, promote formation microbial biofilms. These biofilms have varied metabolic actions govern subsequent succession micro- meso-organisms habitation MPs. The assemblage ecosystems colonising environment is often referred to “plastisphere”. Polymer type, environmental conditions, nutrient status, salinity, season, affect composition biofilm. Microbial accelerated by biofilm on particulate plastics enables movement microorganisms, especially environment, impacts transport toxicity contaminants associated with these fragments. This review paper describes processes formation, factors affecting implications mobility degradation MPs, bioavailability

Language: Английский

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Marine Actinomycetes, New Sources of Biotechnological Products

Marine Drugs, Journal Year: 2021, Volume and Issue: 19(7), P. 365 - 365

Published: June 25, 2021

The Actinomycetales order is one of great genetic and functional diversity, including diversity in the production secondary metabolites which have uses medical, environmental rehabilitation, industrial applications. Secondary produced by actinomycete species are an abundant source antibiotics, antitumor agents, anthelmintics, antifungals. These actinomycete-derived medicines circulation as current treatments, but actinomycetes also being explored potential sources new compounds to combat multidrug resistance pathogenic bacteria. Actinomycetes a solve concerns another area recent investigation, particularly their utility bioremediation pesticides, toxic metals, radioactive wastes, biofouling. Other applications include biofuels, detergents, food preservatives/additives. Exploring other unique properties will allow for deeper understanding this interesting taxonomic group. Combined with engineering, microbial experimental evolution, enhancement techniques, it reasonable assume that use marine continue increase. Novel products begin be developed diverse applied research purposes, zymology enology. This paper outlines knowledge usage research, focusing on isolates providing direction future research.

Language: Английский

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Insights into biodegradation of antibiotics during the biofilm-based wastewater treatment processes

Journal of Cleaner Production, Journal Year: 2023, Volume and Issue: 393, P. 136321 - 136321

Published: Feb. 4, 2023

Language: Английский

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Microbial Biofilms in Urinary Tract Infections and Prostatitis: Etiology, Pathogenicity, and Combating strategies

Pathogens, Journal Year: 2016, Volume and Issue: 5(4), P. 65 - 65

Published: Nov. 30, 2016

Urinary tract infections (UTIs) are one of the most important causes morbidity and health care spending affecting persons all ages. Bacterial biofilms play an role in UTIs, responsible for persistent leading to recurrences relapses. UTIs associated with microbial developed on catheters account a high percentage nosocomial common source Gram-negative bacteremia hospitalized patients. The purpose this mini-review is present etiology female UTI different male prostatitis syndromes, their consequences, as well challenges therapy.

Language: Английский

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Extracellular-matrix-mediated osmotic pressure drives Vibrio cholerae biofilm expansion and cheater exclusion

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: Aug. 15, 2017

Biofilms, surface-attached communities of bacteria encased in an extracellular matrix, are a major mode bacterial life. How the material properties matrix contribute to biofilm growth and robustness is largely unexplored, particular response environmental perturbations such as changes osmotic pressure. Here, using Vibrio cholerae our model organism, we show that during active cell growth, production enables biofilm-dwelling cells establish pressure difference between external environment. This promotes expansion on nutritious surfaces by physically swelling colony, which enhances nutrient uptake, matrix-producing outcompete non-matrix-producing cheaters via physical exclusion. Osmotic together with crosslinking also controls submerged biofilms their susceptibility invasion planktonic cells. As basic physicochemical principles universal, findings may have implications for other biofilm-forming species.Most live biofilms, matrix. Yan et al. increases within biofilm, promoting exclusion non-matrix producing cheaters.

Language: Английский

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Symbiosis in eukaryotic evolution

Journal of Theoretical Biology, Journal Year: 2017, Volume and Issue: 434, P. 20 - 33

Published: Feb. 28, 2017

Language: Английский

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