Heat stress drives transcription of LTR retrotransposons in the regenerative flatwormMacrostomum lignano DOI Creative Commons
Kirill Ustyantsev, Stijn Mouton, Mikhail V. Biryukov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Abstract The evolutionary arms race between transposable elements (TEs) and their hosts contributes to genomic complexity. As TEs mobilization is deleterious for individual cells organisms, activity restricted. During stress, can be reactivated; however, the exact mechanisms vary. We discovered that in flatworm Macrostomum lignano , LTR retrotransposons hijack heat shock response pathway boost transcription at elevated temperatures. While it has been well-described cruciferous plants, this first report of mechanism animal retrotransposons. Our results suggest a convergent evolution stress from animals plants.

Language: Английский

Developmental onset of planarian whole-body regeneration depends on axis reset DOI Creative Commons
C. Booth,

B STEVENS,

Clover A. Stubbert

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
One probe fits all: a highly customizable modular RNA in situ hybridization platform expanding the application of SABER DNA probes DOI Creative Commons
Kirill Ustyantsev, Mattia Stranges, Filippo Volpe

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 23, 2024

ABSTRACT In situ hybridization (ISH) of RNA is a key method to visualize gene expression patterns in complex biological samples. The technique indispensable for research related e.g. development, disease, function, and the validation novel cell types identified by single-cell sequencing methods. Especially non-mammalian models lacking accessibility broad spectrum antibodies, ISH remains major tool. Diverse available protocols require different custom probe types, design, and/or proprietary signal detection chemistry. This makes it hard navigate beginner increases costs when multiple methods need be applied. Here, we describe OneSABER – unified open platform connecting commonly used canonical recently developed single- multiplex, colorimetric, fluorescent approaches. uses single type DNA probes adapted from amplification exchange reaction (SABER) method. We demonstrate applications proposed framework whole-mount samples regenerative flatworm Macrostomum lignano , advancing this animal as powerful model stem regeneration research.

Language: Английский

Citations

0
Exploring plant and microbial antimicrobials for sustainable public health and environmental preservation DOI Creative Commons

Mayuri Saini,

Baljeet Singh Saharan, Satish Kumar

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 5, 2024

Language: Английский

Citations

0
Macrostomum lignano Complements the Portfolio of Simple Animal Models Used for Marine Toxicological Studies DOI Open Access
Yuan‐Yuan Ma, Thomas Roeder

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13092 - 13092

Published: Dec. 5, 2024

is gaining increasing recognition as a model organism for toxicological studies in marine ecosystems and expands the range of simple animal models currently used. Water pollution caused by human activities not only endangers environmental integrity but also affects health, underlining need to monitor water effectively. This review describes distinctive characteristics

Language: Английский

Citations

0
Heat stress drives transcription of LTR retrotransposons in the regenerative flatwormMacrostomum lignano DOI Creative Commons
Kirill Ustyantsev, Stijn Mouton, Mikhail V. Biryukov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Abstract The evolutionary arms race between transposable elements (TEs) and their hosts contributes to genomic complexity. As TEs mobilization is deleterious for individual cells organisms, activity restricted. During stress, can be reactivated; however, the exact mechanisms vary. We discovered that in flatworm Macrostomum lignano , LTR retrotransposons hijack heat shock response pathway boost transcription at elevated temperatures. While it has been well-described cruciferous plants, this first report of mechanism animal retrotransposons. Our results suggest a convergent evolution stress from animals plants.

Language: Английский

Citations

0