The Landscape of the DNA Transposons in the Genome of the Horezu_LaPeri Strain of Drosophila melanogaster DOI Creative Commons
Alexandru Marian Bologa, Ileana Stoica, Nicoleta Denisa Constantin

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(6), P. 494 - 494

Published: May 25, 2023

Natural transposons (NTs) represent mobile DNA sequences found in both prokaryotic and eukaryotic genomes. Drosophila melanogaster (the fruit fly) is a model organism with NTs standing for about 20% of its genome has contributed significantly to the understanding various aspects transposon biology. Our study describes an accurate approach designed map class II (DNA transposons) Horezu_LaPeri fly strain, consecutive Oxford Nanopore Technology sequencing. A whole bioinformatics analysis was conducted using Genome ARTIST_v2, LoRTE RepeatMasker tools identify insertions. Then, gene ontology enrichment performed order evaluate potential adaptive role some Herein, we describe insertions specific predictive functional insertional alleles. The PCR validation P-element this along putative consensus sequence KP element, also reported. Overall, strain contains several associated genes known be involved processes. For these genes, alleles obtained via mobilization artificial were previously This very alluring aspect, as it suggests that mutagenesis experiments conducting predictions laboratory strains may confirmed by mirroring which are expected at least natural strains.

Language: Английский

On the origin of the P-element invasion in Drosophila simulans DOI Creative Commons
Filip Wierzbicki, Riccardo Pianezza, Divya Selvaraju

et al.

Mobile DNA, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 26, 2025

Language: Английский

Citations

0
The Transposition Rate Has Little Influence on the Plateauing Level of the P-element DOI Creative Commons
Robert Kofler, Viola Nolte, Christian Schlötterer

et al.

Molecular Biology and Evolution, Journal Year: 2022, Volume and Issue: 39(7)

Published: June 22, 2022

The popular trap model assumes that the invasions of transposable elements (TEs) in mammals and invertebrates are stopped by piRNAs emerge after insertion TE into a piRNA cluster. It remains, however, still unclear which factors influence dynamics invasions. activity (i.e., transposition rate) is one frequently discussed key factor. Here we take advantage temperature-dependent P-element, widely studied eukaryotic TE, to test how affects invasion. We monitored P-element invasion experimental Drosophila simulans populations at hot cold culture conditions. Despite marked differences rates, reached very similar copy numbers both temperatures. reduction rate upon approaching number plateau was accompanied amounts against Nevertheless, also observed fewer insertions clusters than expected, not compatible with simple model. ping-pong cycle, degrades transcripts, becomes typically active plateaued. generated model, few parameters, largely captures dynamics. conclude has most only minor on abundance, but other factors, such as paramutations or selection shaping composition.

Language: Английский

Citations

18
Associated technologies for genome editing DOI
Kui Li, Yulian Mu, Bingyuan Wang

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 95 - 205

Published: Jan. 1, 2024

Language: Английский

Citations

1
The Landscape of the DNA Transposons in the Genome of the Horezu_LaPeri Strain of Drosophila melanogaster DOI Creative Commons
Alexandru Marian Bologa, Ileana Stoica, Nicoleta Denisa Constantin

et al.

Insects, Journal Year: 2023, Volume and Issue: 14(6), P. 494 - 494

Published: May 25, 2023

Natural transposons (NTs) represent mobile DNA sequences found in both prokaryotic and eukaryotic genomes. Drosophila melanogaster (the fruit fly) is a model organism with NTs standing for about 20% of its genome has contributed significantly to the understanding various aspects transposon biology. Our study describes an accurate approach designed map class II (DNA transposons) Horezu_LaPeri fly strain, consecutive Oxford Nanopore Technology sequencing. A whole bioinformatics analysis was conducted using Genome ARTIST_v2, LoRTE RepeatMasker tools identify insertions. Then, gene ontology enrichment performed order evaluate potential adaptive role some Herein, we describe insertions specific predictive functional insertional alleles. The PCR validation P-element this along putative consensus sequence KP element, also reported. Overall, strain contains several associated genes known be involved processes. For these genes, alleles obtained via mobilization artificial were previously This very alluring aspect, as it suggests that mutagenesis experiments conducting predictions laboratory strains may confirmed by mirroring which are expected at least natural strains.

Language: Английский

Citations

0