Genetic and Epigenetic Changes in Arabidopsis thaliana Exposed to Ultraviolet-C Radiation Stress for 25 Generations

Life, Journal Year: 2025, Volume and Issue: 15(3), P. 502 - 502

Published: March 20, 2025

Continuous exposure to stress contributes species diversity and drives microevolutionary processes. It is still unclear, however, whether epigenetic changes, in the form of epimutations such as, for example, differential DNA methylation, are pre-requisite speciation events. We hypothesized that continuous would increase a higher extent than genetic diversity. In this work, we have analyzed effect 25 consecutive generations UV-C-stress on Arabidopsis thaliana genome epigenome. found no evidence increased tolerance UV-C progeny UV-C-stressed plants (F25UV) as compared control (F25C). Genetic analysis showed an number single nucleotide polymorphisms (SNPs) deletions F25UV plants. Most common SNPs were mutations cytosines, C T, A, G. Analysis cytosine methylation significant percentage methylated cytosines at CG context F25C or F2C (parental control). The most differences between either group observed CHG CHH contexts; hypomethylated contexts was over 10 times F25UC group. clustered separately from other groups both genomic epigenomic analyses. GO term differentially genes revealed enrichments “DNA RNA metabolism”, “response stress”, biotic abiotic stimulus”, “signal transduction”. Our work thus demonstrates increases progeny, with changes occurring many stress-responsive pathways.

Language: Английский

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Epigenetic regulation and beyond in grapevine‐pathogen interactions: a biotechnological perspective

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Abstract As one of the most important crop plants worldwide, understanding mechanisms underlying grapevine response to pathogen attacks is key achieving a productive and sustainable viticulture. Recently, epigenetic regulation in plant immunity has gained significant traction scientific community, not only for its role gene expression but also heritability, giving it enormous biotechnological potential. Epigenetic marks have been shown be dynamically modulated genomic regions upon infection, with some being maintained after such, responsible priming defense genes. In grapevine, however, knowledge still limited, especially regarding biotic stress responses, representing glaring gap this plant. Here, we report integrate current on as well non‐epigenetic non‐coding RNAs stress. We explore how may useful breeding resistance, considering different approaches, from uncovering exploiting natural variation inducing through means.

Language: Английский

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Drought stress memory in maize: understanding and harnessing the past for future resilience

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(5)

Published: April 25, 2025

Language: Английский

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Transgressive and parental dominant gene expression and cytosine methylation during seed development in Brassica napus hybrids

Theoretical and Applied Genetics, Journal Year: 2023, Volume and Issue: 136(5)

Published: April 18, 2023

Transcriptomic and epigenomic profiling of gene expression small RNAs during seed seedling development reveals methylation dominance levels with implications on early stage heterosis in oilseed rape. The enhanced performance hybrids through remains a key aspect plant breeding; however, the underlying mechanisms are still not fully elucidated. To investigate potential role transcriptomic patterns hybrid vigor, we investigated expression, RNA abundance genome-wide from two distant Brassica napus ecotypes developmental stages using next-generation sequencing. A total 31117, 344, 36229 7399 differentially expressed genes, microRNAs, interfering methylated regions were identified, respectively. Approximately 70% or features displayed parental where followed same as parents. Via ontology enrichment microRNA-target association analyses development, found copies reproductive, meiotic genes transgressive paternal patterns. Interestingly, maternal was more prominent hypermethylated downregulated formation, contrasting to general gamete demethylation reported gametogenesis angiosperms. Associations between allowed identification putative epialleles diverse pivotal biological functions formation. Furthermore, most regions, siRNAs transposable elements that flanked without differential expression. This suggests may help maintain context. Differential formation an F1 provide novel insights into roles heterosis.

Language: Английский

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An emerging role beyond genetics: DNA methylation in horticultural quality shaping

The Innovation Life, Journal Year: 2024, Volume and Issue: 2(1), P. 100050 - 100050

Published: Jan. 1, 2024

<p>Horticultural products play an increasingly vital role in addressing the nutritional needs of world’s expanding population, which has surpassed 8 billion. The global trend towards health-oriented diets motivated consumers to seek high-quality natural horticultural food consumption. This highlights pressing requirement for updated guidance and strategies sustainable quality upgrading. Meanwhile, DNA methylation, epigenetic modification having transcriptional-regulation potential, is emerging as a crucial dominator food. In this current investigation, we integrate valuable methylation loci regulating traits fruit vegetable, elucidating underlying mechanisms emphasizing impressive species-specificity. At early stage, most extensively studied aspects include promoter transposable elements. Additionally, delve into locus-specific methylation-editing techniques, whose achievable genetic-modification-free advantages are promising alleviate consumer concerns regarding genetic-modification products. Overall, review devoted providing insights development produce design response security challenges.</p>

Language: Английский

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