Multiple paths towards repeated phenotypic evolution in the spiny-leg adaptive radiation (Tetragnatha; Hawaii) DOI Creative Commons
José Cerca, Darko D. Cotoras, Cindy G. Santander

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 2, 2022

Abstract The repeated evolution of phenotypes is ubiquitous in nature and offers some the clearest evidence role natural selection evolution. genomic basis phenotypic often complex can arise from a combination gene flow, shared ancestral polymorphism de novo mutation. Here, we investigate ecomorph adaptive radiation Hawaiian spiny-leg Tetragnatha . This comprises four ecomorphs that are microhabitat-specialists, differ body pigmentation size (Green, Large Brown, Maroon, Small Brown). Using 76 newly generated low-coverage, whole-genome resequencing samples, coupled with population phylogenomic tools, studied evolutionary history to understand lineage genetic underpinnings Congruent previous works, find each has evolved twice, exception Brown ecomorph, which three times. Maroon likely involved hybridization events, whereas Green because either standing variation or Pairwise comparisons based on fixation index (F ST ) show divergent regions include genes functions associated (melanization), learning, neuronal synapse activity, circadian rhythms. These results linked multiple suggests previously unknown learning rhythms ecomorph.

Language: Английский

Pronounced expression of extracellular matrix proteoglycans regulated by Wnt pathway underlies the parallel evolution of lip hypertrophy in East African cichlids DOI Creative Commons
Nagatoshi Machii, Ryo Hatashima, Tatsuya Niwa

et al.

Published: April 28, 2024

Abstract Cichlid fishes inhabiting the East African Great Lakes, Victoria, Malawi, and Tanganyika, are textbook examples of parallel evolution, as they have acquired similar traits independently in each three lakes during process adaptive radiation. In particular, “hypertrophied lip” has been highlighted a prominent example evolution. However, underlying molecular mechanisms remain poorly understood. this study, we conducted an integrated comparative analysis between hypertrophied normal lips cichlids across based on histology, proteomics, transcriptomics. Histological proteomic analyses revealed that were characterized by enlargement proteoglycan-rich layer, which versican periostin proteins abundant. Transcriptome expression extracellular matrix-related genes, including collagens, glycoproteins proteoglycans, was higher lips, regardless their phylogenetic relationships. addition, Wnt signaling pathway, is involved promoting proteoglycan expression, highly expressed both juvenile adult stages lips. Our comprehensive showed different origins can be explained transcriptomic profiles, may provide important clues into phenotypic parallelisms cichlids.

Language: Английский

Citations

0
Genetic relationships between and within some Malawian cichlid genera DOI
Shaharior Hashem, Koichiro Kawai,

T. Kushida

et al.

Journal of Fish Biology, Journal Year: 2024, Volume and Issue: 105(3), P. 931 - 943

Published: June 24, 2024

Genetic relationships were examined for a total of 90 individuals species or var. from 13 genera Malawian cichlids based on the sequences an amplified 991-bp fragment mtDNA control region (mtDNA-CR). In network analysis, no exclusive clades made by all members any in this study. However, congeneric observed Buccochromis, Copadichromis, Protomelas, and Sciaenochromis, whereas Mylochromis, Nimbochromis, Otopharynx. non-mbuna, Aulonocara-Lethrinops group was divided into two groups, mean genetic distance larger much lower mbuna than other non-mbuna. Overall within genus generally low mbuna, it relatively high tree each genus, more large observed, some clades, such as those Aulonocara hansbaenschi nyassae Aulonocara, Lethrinops micrentodon sp. "gold harbor" Lethrinops, Otopharynx ovatus brooksi Otopharynx, very deeply differentiated. Besides, species, Pseudotropheus crabro, extremely differentiated genus. These results suggest widespread morphological convergence across taxa parallel with deep differentiation long evolutionary story possibility generation hybridization small non-mbuna species. Furthermore, taxonomical reexamination is necessary strong support connection.

Language: Английский

Citations

0
Pronounced expression of extracellular matrix proteoglycans regulated by Wnt pathway underlies the parallel evolution of lip hypertrophy in East African cichlids DOI Open Access
Nagatoshi Machii, Ryo Hatashima, Tatsuya Niwa

et al.

Published: Sept. 5, 2024

Cichlid fishes inhabiting the East African Great Lakes, Victoria, Malawi, and Tanganyika, are textbook examples of parallel evolution, as they have acquired similar traits independently in each three lakes during process adaptive radiation. In particular, “hypertrophied lip” has been highlighted a prominent example evolution. However, underlying molecular mechanisms remain poorly understood. this study, we conducted an integrated comparative analysis between hypertrophied normal lips cichlids across based on histology, proteomics, transcriptomics. Histological proteomic analyses revealed that were characterized by enlargement proteoglycan-rich layer, which versican periostin proteins abundant. Transcriptome expression extracellular matrix-related genes, including collagens, glycoproteins proteoglycans, was higher lips, regardless their phylogenetic relationships. addition, genes Wnt signaling pathway, is involved promoting proteoglycan expression, highly expressed both juvenile adult stages lips. Our comprehensive showed different origins can be explained transcriptomic profiles, may provide important clues into phenotypic parallelisms cichlids.

Language: Английский

Citations

0
Pronounced expression of extracellular matrix proteoglycans regulated by Wnt pathway underlies the parallel evolution of lip hypertrophy in East African cichlids DOI Open Access
Nagatoshi Machii, Ryo Hatashima, Tatsuya Niwa

et al.

Published: Sept. 5, 2024

Cichlid fishes inhabiting the East African Great Lakes, Victoria, Malawi, and Tanganyika, are textbook examples of parallel evolution, as they have acquired similar traits independently in each three lakes during process adaptive radiation. In particular, “hypertrophied lip” has been highlighted a prominent example evolution. However, underlying molecular mechanisms remain poorly understood. this study, we conducted an integrated comparative analysis between hypertrophied normal lips cichlids across based on histology, proteomics, transcriptomics. Histological proteomic analyses revealed that were characterized by enlargement proteoglycan-rich layer, which versican periostin proteins abundant. Transcriptome expression extracellular matrix-related genes, including collagens, glycoproteins proteoglycans, was higher lips, regardless their phylogenetic relationships. addition, Wnt signaling pathway, is involved promoting proteoglycan expression, highly expressed both juvenile adult stages lips. Our comprehensive showed different origins can be explained transcriptomic profiles, may provide important clues into phenotypic parallelisms cichlids.

Language: Английский

Citations

0
Population genomic analysis reveals cryptic population structure in the commercially important Lake Malawi cichlid Copadichromis mloto (IIes, 1960) DOI
Wilson Sawasawa, Alexander Hooft van Huysduynen, Sophie Gresham

et al.

Hydrobiologia, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Language: Английский

Citations

0
A whole-body micro-CT scan library that captures the skeletal diversity of Lake Malawi cichlid fishes DOI Creative Commons

Callum V Bucklow,

Martin J. Genner, George F. Turner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 1, 2023

ABSTRACT Here we describe a dataset of freely available, readily processed, whole-body μ CT-scans 56 species (116 specimens) Lake Malawi cichlid fishes that captures considerable majority the morphological variation present in this remarkable adaptive radiation. We contextualise scanned specimens within discussion their respective ecomorphological groupings and suggest possible macroevolutionary studies could be conducted with these data. also methodology to efficiently CT-scan (on average) 23 per hour, limiting scanning time alleviating financial cost whilst maintaining high resolution. demonstrate utility method by reconstructing 3D models multiple bones from dataset. hope will enable further study fascinating system permit wider-scale comparisons other radiations.

Language: Английский

Citations

0
A new species of deep-water Lethrinops (Cichlidae) from Lake Malawi DOI Creative Commons
George F. Turner

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: May 2, 2022

Abstract A new species of cichlid fish, Lethrinops atrilabris is described from specimens collected by trawling at a depth around 90m off Monkey Bay, southern Lake Malawi. It assigned to the genus on basis its vertical flank barring, lack enlarged cephalic lateral line canal pores and form lower jaw dental arcade. can be distinguished congeneric male breeding dress contrasting barring dark ventral surface, most strikingly lips, throat chest, relatively small known maximum size (<75mm SL), large eyes (38-41% head length), laterally compressed body (depth 2.5-2.7 times max width) gillraker count (13-14).

Language: Английский

Citations

0
Multiple paths towards repeated phenotypic evolution in the spiny-leg adaptive radiation (Tetragnatha; Hawaii) DOI Creative Commons
José Cerca, Darko D. Cotoras, Cindy G. Santander

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Dec. 2, 2022

Abstract The repeated evolution of phenotypes is ubiquitous in nature and offers some the clearest evidence role natural selection evolution. genomic basis phenotypic often complex can arise from a combination gene flow, shared ancestral polymorphism de novo mutation. Here, we investigate ecomorph adaptive radiation Hawaiian spiny-leg Tetragnatha . This comprises four ecomorphs that are microhabitat-specialists, differ body pigmentation size (Green, Large Brown, Maroon, Small Brown). Using 76 newly generated low-coverage, whole-genome resequencing samples, coupled with population phylogenomic tools, studied evolutionary history to understand lineage genetic underpinnings Congruent previous works, find each has evolved twice, exception Brown ecomorph, which three times. Maroon likely involved hybridization events, whereas Green because either standing variation or Pairwise comparisons based on fixation index (F ST ) show divergent regions include genes functions associated (melanization), learning, neuronal synapse activity, circadian rhythms. These results linked multiple suggests previously unknown learning rhythms ecomorph.

Language: Английский

Citations

0