Horizontal transfer and the widespread presence of Galileo transposons in Drosophilidae (Insecta: Diptera) DOI Creative Commons
Henrique R.M. Antoniolli, Sebastián Pita, Maríndia Deprá

et al.

Genetics and Molecular Biology, Journal Year: 2023, Volume and Issue: 46(3 suppl 1)

Published: Jan. 1, 2023

Galileo is a transposon notoriously involved with inversions in Drosophila buzzatii by ectopic recombination. Although widespread Drosophila, little known about this other lineages of Drosophilidae. Here, the abundance canonical and its evolutionary history Drosophilidae genomes was estimated reconstructed across genera within two subfamilies. Sequences were masked these their transposase sequences recovered using BLASTn. Phylogenetic analyses employed to reconstruct compare it that host genomes. found nearly all 163 species, however, only 37 harbored complete sequences. In remaining, highly fragmented. Copies from related species clustered, however horizontal transfer events detected between melanogaster montium groups latter Lordiphosa genus. The similarity virilis willistoni be consequence lineage sorting. Therefore, evolution primarily marked vertical transmission long-term inactivation, mainly through deletion open reading frames. has potential lead copies become miniature inverted-repeat transposable elements.

Language: Английский

Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs DOI Creative Commons
Tarcísio Fontenele de Brito, Maira Arruda Cardoso, Nazerke Atinbayeva

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 20, 2024

Piwi proteins and the associated Piwi-interacting RNAs (piRNAs) coordinate a surveillance system that protects animal genome from DNA damage induced by transposable element (TE) mobilization. While pathway has been described in detail fruit fly

Language: Английский

Citations

2
Embryonic piRNAs target horizontally transferred vertebrate transposons in assassin bugs DOI Open Access
Tarcísio Fontenele de Brito, Maira Arruda Cardoso, Nazerke Atinbayeva

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 24, 2024

Abstract Piwi proteins and the associated Piwi-interacting RNAs (piRNAs) coordinate a surveillance system that protects animal genome from DNA damage induced by transposable element (TE) mobilization. While pathway has been described in detail fruit fly Drosophila melanogaster, much less is known more basal insects. Here, we investigated adaptation of piRNA to horizontally transferred transposons (HTTs) assassin bug Rhodnius prolixus , primary vector Chagas disease. acquired specific classes HTTs feeding on bats, opossums squirrel monkeys. By analyzing temporal dynamics cluster expression production during critical stages development, show peak levels ∼28 nt long piRNAs correlate with reduced HTT resident TE primarily embryogenesis. Strikingly, while TEs seem engage typical ping-pong amplification mechanism, sense antisense instead overlap ∼20 or do not display signatures. These features are explained at least part low number copies inserted into clusters might point non-canonical mechanism biogenesis. Our data reveal piRNA, but siRNA pathway, responded were recently vertebrate tetrapods hematophagous insect medical relevance.

Language: Английский

Citations

1
Phylogenetic relatedness rather than aquatic habitat fosters horizontal transfer of transposable elements in animals DOI Creative Commons
Héloise Müller, Rosina Savisaar, Jean Peccoud

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 21, 2024

Abstract Horizontal transfer of transposable elements (HTT) is an important driver genome evolution, yet the factors conditioning this phenomenon remain poorly characterized. Here, we screened 247 animal genomes from four phyla (annelids, arthropods, mollusks, chordates), spanning 19 independent transitions between aquatic and terrestrial lifestyles, to evaluate suspected positive effects habitat phylogenetic relatedness on HTT. Among 5,952 HTT events recovered, vast majority (>85%) involve DNA transposons, which Mariner-like hAT-like have highest rates horizontal transfer, intra-genomic amplification. Using a novel approach that circumvents putative biases linked inertia taxon sampling, found positively correlate with similarity in type but were not significantly higher than animals. However, modelling number as function divergence time Bayesian framework revealed clear effect most species studied (162 out 247). The very pronounced: typical expected show 10 times more transfers it diverged 125 million years (My) ago 375 My ago. Overall, our study underscores pervasiveness throughout animals impact evolutionary its dynamics. Significance statement Genetic material can be transmitted organisms through other means reproduction, process called transfer. mechanisms underlying unclear, although often involves (TEs). TEs are segments capable jumping within genomes, also occasionally individuals. evidence for nearly 6,000 among animals, based genomic comparisons annelids, chordates mollusks. Contrarily expectations, no excess versus By contrast, analyzed appeared engaged many close distant relatives, highlighting strong TEs.

Language: Английский

Citations

0
Horizontal transfer and the widespread presence of Galileo transposons in Drosophilidae (Insecta: Diptera) DOI Creative Commons
Henrique R.M. Antoniolli, Sebastián Pita, Maríndia Deprá

et al.

Genetics and Molecular Biology, Journal Year: 2023, Volume and Issue: 46(3 suppl 1)

Published: Jan. 1, 2023

Galileo is a transposon notoriously involved with inversions in Drosophila buzzatii by ectopic recombination. Although widespread Drosophila, little known about this other lineages of Drosophilidae. Here, the abundance canonical and its evolutionary history Drosophilidae genomes was estimated reconstructed across genera within two subfamilies. Sequences were masked these their transposase sequences recovered using BLASTn. Phylogenetic analyses employed to reconstruct compare it that host genomes. found nearly all 163 species, however, only 37 harbored complete sequences. In remaining, highly fragmented. Copies from related species clustered, however horizontal transfer events detected between melanogaster montium groups latter Lordiphosa genus. The similarity virilis willistoni be consequence lineage sorting. Therefore, evolution primarily marked vertical transmission long-term inactivation, mainly through deletion open reading frames. has potential lead copies become miniature inverted-repeat transposable elements.

Language: Английский

Citations

0