Heteromorphic ZZ/ZW sex chromosomes sharing gene content with mammalian XX/XY are conserved in Madagascan chameleons of the genus Furcifer DOI Creative Commons
Michail Rovatsos, Sofia Mazzoleni, Barbora Augstenová

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 6, 2023

Abstract Chameleons are well-known lizards with unique morphology and physiology, but their sex determination has remained poorly studied. Madagascan chameleons of the genus Furcifer have cytogenetically distinct Z W chromosomes occasionally 1 2 /Z multiple neo-sex chromosomes. To identify gene content chromosomes, we microdissected sequenced F. cf. oustaleti (ZZ/ZW) pardalis (Z W). In addition, genomes a male female lateralis performed comparative coverage analysis between sexes. Despite notable heteromorphy distinctiveness in heterochromatin content, share approximately 90% content. This finding demonstrates poor correlation degree differentiation at cytogenetic sequence level. The test homology based on comparison copy number variation revealed that heterogamety differentiated stable for least 20 million years. These co-opted role same genomic region as viviparous mammals, lacertids geckos Paroedura , which makes these groups excellent model studies convergent divergent evolution

Language: Английский

The first complete assembly for a lungless urodelan with a "miniaturized" genome, the Northern Dusky Salamander (Plethodontidae: Desmognathus fuscus) DOI Creative Commons
Edward A. Myers, R. Alexander Pyron

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 2, 2024

Salamanders have some of the largest genomes among animals. However, there is a great disparity in total size, ranging from ~8-120GB depending on lineage. Species lungless genus Desmognathus (Plethodontidae) are smallest, with estimated genome sizes 13-15GB. Salamander exceptional interest numerous topics genome-size evolution, genetic basis evolutionary differences life history, and physiological regeneration, vision, immunity. their large limited previous attempts at sequencing assembly, particularly given difficulties mapping extensive repeat regions short-read data. Here we assemble draft fuscus using PacBio HiFi reads generate transcriptomic data two specimens. The combined assembly resulted 16.1GB 19,640 contigs an N/L50 2,455/1.75MB, longest contig 27.9MB. transcriptome nearly complete 93% 5,310 BUSCO tetrapod orthologs identified. Attempts to scaffold these existing Ambystoma only 5.8GB D. this reference. This low success suggests substantial syntenic sequence divergence across salamanders, which may be result significant miniaturization genome. Identification annotation transposable elements reveals that 26% single copy 74% corresponding TEs. most common class TE LTRs (35% genome) LINEs (~15% genome). relative landscape TEs shows early expansion slow contraction LINEs, followed by quick recent both DNA transposons. will serve as important reference for amphibian genomics.

Language: Английский

Citations

3
Heteromorphic ZZ/ZW sex chromosomes sharing gene content with mammalian XX/XY are conserved in Madagascan chameleons of the genus Furcifer DOI Creative Commons
Michail Rovatsos, Sofia Mazzoleni, Barbora Augstenová

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Feb. 28, 2024

Abstract Chameleons are well-known lizards with unique morphology and physiology, but their sex determination has remained poorly studied. Madagascan chameleons of the genus Furcifer have cytogenetically distinct Z W chromosomes occasionally 1 2 /Z multiple neo-sex chromosomes. To identify gene content chromosomes, we microdissected sequenced F. oustaleti (ZZ/ZW) pardalis (Z W). In addition, genomes a male female lateralis performed comparative coverage analysis between sexes. Despite notable heteromorphy distinctiveness in heterochromatin content, share approximately 90% content. This finding demonstrates poor correlation degree differentiation at cytogenetic level. The test homology based on comparison copy number variation revealed that heterogamety differentiated stable for least 20 million years. These co-opted role same genomic region as viviparous mammals, lacertids geckos Paroedura , which makes these groups excellent model studies convergent divergent evolution

Language: Английский

Citations

2
Heteromorphic ZZ/ZW sex chromosomes sharing gene content with mammalian XX/XY are conserved in Madagascan chameleons of the genus Furcifer DOI Creative Commons
Michail Rovatsos, Sofia Mazzoleni, Barbora Augstenová

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 6, 2023

Abstract Chameleons are well-known lizards with unique morphology and physiology, but their sex determination has remained poorly studied. Madagascan chameleons of the genus Furcifer have cytogenetically distinct Z W chromosomes occasionally 1 2 /Z multiple neo-sex chromosomes. To identify gene content chromosomes, we microdissected sequenced F. cf. oustaleti (ZZ/ZW) pardalis (Z W). In addition, genomes a male female lateralis performed comparative coverage analysis between sexes. Despite notable heteromorphy distinctiveness in heterochromatin content, share approximately 90% content. This finding demonstrates poor correlation degree differentiation at cytogenetic sequence level. The test homology based on comparison copy number variation revealed that heterogamety differentiated stable for least 20 million years. These co-opted role same genomic region as viviparous mammals, lacertids geckos Paroedura , which makes these groups excellent model studies convergent divergent evolution

Language: Английский

Citations

0