Protein Homeostasis Impairment Alters Phenotypic Heterogeneity of Biofilm Communities DOI Creative Commons

Judith Matavacas,

Claes von Wachenfeldt

Molecular Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT Biofilms are highly organized, cooperating communities of microorganisms encased in a self‐produced extracellular matrix, providing resilience against external stress such as antimicrobial agents and host defenses. A hallmark biofilms is their phenotypic heterogeneity, which enhances the overall growth survival community. In this study, we demonstrate that removing dnaK tig genes encoding core molecular chaperones DnaK (Hsp70 homolog) Trigger factor disrupted protein homeostasis Bacillus subtilis resulted formation an extremely mucoid biofilm with aberrant architecture, compromised structural integrity, altered heterogeneity. These changes include large reduction motile subpopulation overrepresentation matrix producers endospores. Overproduction poly‐γ‐glutamic acid contributed crucially to phenotype architecture. Homeostasis impairment, triggered by elevated temperatures, wild‐type cells led phenotypes similar those observed strains lacking both . Our findings show disruption homeostasis, whether due absence or because environmental factors, severely features.

Language: Английский

Protein Homeostasis Impairment Alters Phenotypic Heterogeneity of Biofilm Communities DOI Creative Commons

Judith Matavacas,

Claes von Wachenfeldt

Molecular Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT Biofilms are highly organized, cooperating communities of microorganisms encased in a self‐produced extracellular matrix, providing resilience against external stress such as antimicrobial agents and host defenses. A hallmark biofilms is their phenotypic heterogeneity, which enhances the overall growth survival community. In this study, we demonstrate that removing dnaK tig genes encoding core molecular chaperones DnaK (Hsp70 homolog) Trigger factor disrupted protein homeostasis Bacillus subtilis resulted formation an extremely mucoid biofilm with aberrant architecture, compromised structural integrity, altered heterogeneity. These changes include large reduction motile subpopulation overrepresentation matrix producers endospores. Overproduction poly‐γ‐glutamic acid contributed crucially to phenotype architecture. Homeostasis impairment, triggered by elevated temperatures, wild‐type cells led phenotypes similar those observed strains lacking both . Our findings show disruption homeostasis, whether due absence or because environmental factors, severely features.

Language: Английский

Citations

0