Multiple Isoforms of the Activin-like receptor Baboon differentially regulate proliferation and conversion behaviors of neuroblasts and neuroepithelial cells in theDrosophilalarval brain DOI Creative Commons
Gyunghee Lee, Aidan J. Peterson, Myung-Jun Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 9, 2024

Abstract In Drosophila coordinated proliferation of two neural stem cells, neuroblasts (NB) and neuroepithelial (NE) is pivotal for proper larval brain growth that ultimately determines the final size performance an adult brain. The displays phases based on behaviors NB NEs: first one in early stages, influenced by nutritional status second last stage, promoted ecdysone signaling after critical weight checkpoint. Mutations baboon ( babo ) gene produces three isoforms (BaboA-C), all acting as type-I receptors Activin-type transforming factor β (TGF-β) signaling, cause a small phenotype due to severely reduced cells. this study we show loss function affects NBs NEs well conversion from both phases. By analyzing -null newly generated isoform-specific mutants CRISPR mutagenesis RNAi knockdowns cell- stage-specific manner, further demonstrate differential contributions these cellular events with BaboA playing major role. Our data expression EcR-B1 also regulated primarily along other isoforms. Blocking EcR cells results more severe than baboA -knockdown alone. summary, our proposes Babo-mediated promotes achieves upstream phase. Author Summary Evolutionarily conserved TGF-β pathway widely utilized regulator diverse processes development vertebrates invertebrates. A key element Activin type receptor Babo. isoforms, each unique ligand preference. uncovers NEs, together responsible magnitude growth. Three act individually or regulate coordination developmental status. findings emphasize crucial postembryonic neurogenesis generates 90% neuronal population

Language: Английский

Timing Drosophila development through steroid hormone action DOI Creative Commons

Hannah Morrow,

Christen K. Mirth

Current Opinion in Genetics & Development, Journal Year: 2024, Volume and Issue: 84, P. 102148 - 102148

Published: Jan. 24, 2024

Specifically timed pulses of the moulting hormone ecdysone are necessary for developmental progression in insects, guiding development through important milestones such as larval moults, pupation and metamorphosis. It also coordinates acquisition cell identities, known patterning, growth a tissue-specific manner. In absence ecdysone, receptor heterodimer Ecdysone Receptor Ultraspiracle represses expression target primary response genes, which become de-repressed titre rises. However, signalling elicits both repressive activating responses temporal To understand how achieves specificity, this review explores layers gene regulation involved stage-appropriate Drosophila fruit flies.

Language: Английский

Citations

4
The endocuticle structural glycoprotein AgSgAbd‐2‐like is required for cuticle formation and survival in the melon aphid Aphis gossypii DOI Open Access

Mingyu Guo,

Xueting Qu,

Shenhang Cheng

et al.

Insect Science, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

Abstract Cuticular proteins are essential for cuticle formation, molting, and survival in insects. However, functional analysis of cuticular the melon aphid has been limited. In this study, we identified an endocuticle structural glycoprotein (ESG) AgSgAbd‐2‐like Aphis gossypii , which is a member RR‐1 subfamily CPR (cuticular protein containing conserved Rebers–Riddiford motif) chitin‐binding proteins. When double‐stranded RNA delivered epidermally, knocked down, resulting molting defects mortality. The expression comparatively low prior to increases following molting. Ecdysone signaling consistently suppresses . Histologically, whole thinner interference (RNAi) aphids, leading cause Furthermore, knockdown any other homolog ESGs, including AgSgAbd‐4, AgSgAbd‐4‐like, AgSgAbd‐8‐like, AgSgAbd‐9‐like, results death, like that by RNAi. These indicate ESGs formation could be potential targets RNAi‐based pest management.

Language: Английский

Citations

0
Survival, Movement, and Lifespan: Decoding the Roles of Patched‐Related in Drosophila melanogaster DOI Open Access
Cristina Parada, Daniel Prieto

Archives of Insect Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 118(3)

Published: March 1, 2025

ABSTRACT Patched‐related (Ptr) is a transmembrane protein implicated in developmental processes Drosophila melanogaster , yet its precise role remains incompletely understood. Here, we use Ptr 23c null mutants to investigate the functional significance of through entire life cycle monitoring survival during embryonic, larval, pupal and adult development, studying larval locomotion muscle structure. We report that larvae displayed impaired hatching, indicative defective embryonic development. Moreover, mutant exhibited reduced mobility lethargy, suggesting potential involvement neuromuscular function. Morphological analysis somatic muscles revealed enlarged cell nuclei. Despite high preadult mortality, subset adults display an unexpected extension lifespan compared controls, implicating regulation longevity. Our findings provide critical insights into multifaceted highlighting contributions post‐embryonic survival, function, regulation. This study underscores exploring broader genetic networks unravel complexities processes.

Language: Английский

Citations

0
Interactions of juvenile hormone, 20-hydroxyecdysone, developmental genes, and miRNAs during pupal development in Apis mellifera DOI Creative Commons
Thiago S. Depintor, Flávia Cristina de Paula Freitas, Nídia Aparecida Hernandes

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 26, 2025

Abstract Insect development is primarily controlled by juvenile hormone (JH) and 20-hydroxyecdysone (20E), which regulate gene cascades leading to changes in phenotype, physiology, behavior. Besides these hormones, microRNAs play a crucial role insect regulating expression at the post-transcriptional level. To advance molecular understanding of holometabolous developmental events, we investigate pupal phase honeybee, Apis mellifera . In this study, assessed profiles genes components JH 20E – Usp, ftz-f1, EcR, Met, Chd64, InR-2, Kr-h1 Tai as well miRNA-34 miRNA-281 during A. We then analyzed impact treatments on their putative regulators, microRNAs. Overall, selected miRNAs remained stable or were downregulated following treatment, while with JH, upregulated most our candidate Notably, profile Met , an intracellular receptor showed strong correlation fluctuations titers development. Furthermore, computational analysis, followed experimental assays, points both miR-34 miR-281 potential regulators This study paves way for better how hormones interact (miR-34 miR-281) honeybees, elucidating piece complex network interactions.

Language: Английский

Citations

0
Transcriptional induction by ecdysone in Drosophila salivary glands involves an increase in chromatin accessibility and acetylation DOI Creative Commons

А. А. Евдокимова,

Т. Д. Колесникова, M. Yu. Mazina

et al.

Nucleic Acids Research, Journal Year: 2025, Volume and Issue: 53(7)

Published: April 10, 2025

Abstract Transcriptional activation by 20-hydroxyecdysone (20E) in Drosophila provides an excellent model for studying tissue-specific responses to steroids. An increase the 20E concentration regulates degradation of larval and proliferation adult tissues during metamorphosis. To study 20E-dependent transcription, we used natural system controlling concentration—the E23 membrane transporter—which exports from cell. We artificially expressed suppress first wave 20E-inducible transcription at expression revealed a plethora genes salivary glands, while mildly affecting brain. described mechanisms transcriptional glands. depletion decreased binding Pol II TFIID subunit, TBP, promoters primary targets, demonstrating role initiation. At target loci, resulted malfunctioning sites co-bound with EcR CBP/Nejire enriched H3K27Ac mark inherent active enhancers. these sites, was found control chromatin accessibility acetylation. suggest that activity ‘active’ ecdysone-sensitive elements responsible status targets glands wandering larvae.

Language: Английский

Citations

0
Insect nuclear receptors: From orphans to ligands DOI
Wen Liu, Hang Chen, Kirst King‐Jones

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Wolbachia-mediated reduction in the glutamate receptor mGluR promotes female promiscuity and bacterial spread DOI Creative Commons
Brandt Warecki, Giovanni Vega,

Sommer Fowler

et al.

Cell Reports, Journal Year: 2025, Volume and Issue: unknown, P. 115629 - 115629

Published: May 1, 2025

The molecular mechanisms by which parasites mediate host behavioral changes remain largely unexplored. Here, we examine Drosophila melanogaster infected with Wolbachia, a symbiont transmitted through the maternal germline, and find Wolbachia infection increases female receptivity to male courtship hybrid mating. colonize regions of brain that control sense perception behavior. Quantitative global proteomics identify 177 differentially abundant proteins in larval brains. Genetic alteration levels three these adults, metabotropic glutamate receptor mGluR, transcription factor TfAP-2, odorant binding protein Obp99b, each mimic effect on receptivity. Furthermore, >700 are detected Through abundance modeling analyses, distinguish several Wolbachia-produced as potential effectors. These results networks modify behavior promote mating success aid spread Wolbachia.

Language: Английский

Citations

0
From Nanocrystals and Nanocomposites to Microcrystals: The Role of Simonkolleite/ZnO in Overcoming Bacterial Resistance and Ensuring Biocompatibility DOI Creative Commons
Jerusa Maria de Oliveira,

Maria Paula Camargo Costa,

Hugo Félix Perini

et al.

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Language: Английский

Citations

0
Modulation of a critical period for motor development in Drosophila by BK potassium channels DOI Creative Commons
Simon A. Lowe, Abigail D. Wilson, Gabriel Aughey

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(15), P. 3488 - 3505.e3

Published: July 24, 2024

Language: Английский

Citations

3
The Molecular Substrates of Insect Eusociality DOI
Sarah D. Kocher, Callum J. Kingwell

Annual Review of Genetics, Journal Year: 2024, Volume and Issue: 58(1), P. 273 - 295

Published: Aug. 15, 2024

The evolution of eusociality in Hymenoptera—encompassing bees, ants, and wasps—is characterized by multiple gains losses social living, making this group a prime model to understand the mechanisms that underlie behavior complexity. Our review synthesizes insights into evolutionary history molecular basis eusociality. We examine new evidence for key hypotheses pathways regulate behaviors, highlighting convergent on shared toolkit includes insulin/insulin-like growth factor signaling (IIS) target rapamycin (TOR) pathways, juvenile hormone ecdysteroid signaling, epigenetic regulation. emphasize how crosstalk among these nutrient-sensing endocrine enables insects integrate external environmental stimuli, including cues, with internal physiology behavior. argue examining as an integrated regulatory circuit exploring architecture evolves alongside can open door understanding origin complex life histories behaviors group.

Language: Английский

Citations

3