A novel male accessory gland peptide reduces female post-mating receptivity in the brown planthopper DOI Open Access
Yijie Zhang, Ning Zhang, Rongfa Bu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Mating in insects commonly induces a profound change the physiology and behavior of female that serves to secure numerous viable offspring ensure paternity for male by reducing receptivity further mating attempts. Here, we set out characterize post-mating response (PMR) pest insect, brown planthopper (BPH) Nilaparvata lugens identify functional analog sex peptide (SP) and/or other seminal fluid factors contribute PMR Drosophila . We find BPHs display distinct lasts about 4 days includes with decreased males increased oviposition. Extract from accessory glands (MAG) injected into virgin females triggers similar PMR, lasting 24h. Since SP does not exist BPHs, screened candidate mediators performing transcriptional proteomics analysis MAG extract. identified novel 51 amino acid present only BPHs. This peptide, designate maccessin (macc), affects PMR. Females mated macc knockdown wild type second mating, which occur controls. However, oviposition is affected. Injection recombinant reduces receptivity, no effect on Thus, so far promotes Our suggests gene encoding precursor restricted species closely related Author summary In insects, often long-lasting physiology, called (PMR). ensures offspring, but also inhibiting demonstrate , displays egg laying. furthermore extracted gland generates Next, unique (designated maccessin) this responsible reduced affect The appears close relatives N. factor, known induce occurs limited number species.

Language: Английский

Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito DOI Creative Commons
Olivia V. Goldman,

Alexandra E. DeFoe,

Yanyan Qi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Language: Английский

Citations

1
Two neuropeptides that promote blood-feeding inAnopheles stephensimosquitoes DOI Creative Commons
Prashali Bansal,

Roshni Pillai,

DB Pooja

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 15, 2024

Animals routinely need to make decisions about what eat and when. These are influenced not only by the availability quality of food but also internal state animal, which needs compute give weights these different variables before making a choice. Feeding preferences female mosquitoes exemplify this behavioural plasticity. Both male usually feed on carbohydrate-rich sources nectar or sap, feeds blood, is essential for egg development. This blood-appetite modulated across female’s reproductive cycle, yet little known factors that bring it about. We show Anopheles stephensi females, major vector urban Malaria Indian sub-continent West-Africa, suppress blood-feeding between blood-meal until oviposition. Transcriptomics blood-deprived sated An. mid-brains allowed us shortlist several candidate genes might promote behaviour. Through dsRNA-mediated knockdown nine them, we identified two neuropeptides – short Neuropeptide F (sNPF) RYamide blood-feeding. find neuromodulators expressed in non-overlapping subsets neurons brain suggesting behaviour likely multiple mechanisms acting neurons.

Language: Английский

Citations

1
Male Reproductive Glands and Their Secretions in Insects DOI
Ben R. Hopkins, Sarah E. Allen, Frank W. Avila

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

1
Two Neuropeptides that Promote Blood-Feeding in Anopheles Stephensi Mosquitoes DOI

Prashali Bansal,

Roshni Pillai,

Pooja DB

et al.

Published: Jan. 1, 2024

Animals routinely need to make decisions about what eat and when. These are influenced not only by the availability quality of food but also internal state animal, which needs compute give weights these different variables before making a choice. Feeding preferences female mosquitoes exemplify this behavioural plasticity. Both male usually feed on carbohydrate-rich sources nectar or sap, feeds blood, is essential for egg development. This blood-appetite modulated across female's reproductive cycle, yet little known factors that bring it about.We show Anopheles stephensi females, major vector urban Malaria Indian sub-continent West-Africa, suppress blood-feeding between blood-meal until oviposition. Transcriptomics blood-deprived sated An. midbrains allowed us shortlist several candidate genes might promote bloodfeeding behaviour. Through dsRNA-mediated knockdown nine them, we identified two neuropeptides – short Neuropeptide F (sNPF) RYamide - blood-feeding. We find neuromodulators expressed in nonoverlapping subsets neurons brain suggesting behaviour likely multiple mechanisms acting neurons.

Language: Английский

Citations

0
A novel male accessory gland peptide reduces female post-mating receptivity in the brown planthopper DOI Open Access
Yijie Zhang, Ning Zhang, Rongfa Bu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 27, 2024

Abstract Mating in insects commonly induces a profound change the physiology and behavior of female that serves to secure numerous viable offspring ensure paternity for male by reducing receptivity further mating attempts. Here, we set out characterize post-mating response (PMR) pest insect, brown planthopper (BPH) Nilaparvata lugens identify functional analog sex peptide (SP) and/or other seminal fluid factors contribute PMR Drosophila . We find BPHs display distinct lasts about 4 days includes with decreased males increased oviposition. Extract from accessory glands (MAG) injected into virgin females triggers similar PMR, lasting 24h. Since SP does not exist BPHs, screened candidate mediators performing transcriptional proteomics analysis MAG extract. identified novel 51 amino acid present only BPHs. This peptide, designate maccessin (macc), affects PMR. Females mated macc knockdown wild type second mating, which occur controls. However, oviposition is affected. Injection recombinant reduces receptivity, no effect on Thus, so far promotes Our suggests gene encoding precursor restricted species closely related Author summary In insects, often long-lasting physiology, called (PMR). ensures offspring, but also inhibiting demonstrate , displays egg laying. furthermore extracted gland generates Next, unique (designated maccessin) this responsible reduced affect The appears close relatives N. factor, known induce occurs limited number species.

Language: Английский

Citations

0