Chemical Reviews,
Journal Year:
2018,
Volume and Issue:
118(17), P. 8151 - 8187
Published: July 16, 2018
Oligosaccharides
together
with
oligonucleotides
and
oligopeptides
comprise
the
three
major
classes
of
natural
biopolymers.
Automated
systems
for
oligonucleotide
oligopeptide
synthesis
have
significantly
advanced
developments
in
biological
science
by
allowing
nonspecialists
to
rapidly
easily
access
these
Researchers
endeavored
decades
develop
a
comparable
general
automated
system
synthesize
oligosaccharides.
Such
would
revolutionary
impact
on
understanding
roles
glycans
systems.
The
main
challenge
achieving
is
lack
synthetic
methods
routine
glycans.
Currently,
two
homogeneous
glycoconjugates
are
chemical
enzymatic
synthesis.
Enzymatic
glycosylation
can
proceed
stereo-
regiospecifically
without
protecting
group
manipulations.
Moreover,
reaction
conditions
enzyme-catalyzed
glycosylations
extremely
mild
when
compared
glycosylations.
Over
past
few
years
methodology
toward
oligosaccharides
has
been
developed.
Conversely,
while
conceptually
possible,
it
not
as
well
goal
this
survey
provide
foundation
which
continued
technological
advancements
be
made
promote
Chemical Society Reviews,
Journal Year:
2017,
Volume and Issue:
46(16), P. 4895 - 4950
Published: Jan. 1, 2017
The
emerging
inverse
electron
demand
Diels–Alder
(IEDDA)
reaction
stands
out
from
other
bioorthogonal
reactions
by
virtue
of
its
unmatchable
kinetics,
excellent
orthogonality
and
biocompatibility.
Glycobiology,
Journal Year:
2015,
Volume and Issue:
26(2), P. 111 - 128
Published: Oct. 30, 2015
During
malignant
transformation,
glycosylation
is
heavily
altered
compared
with
healthy
tissue
due
to
differential
expression
of
glycosyltransferases,
glycosidases
and
monosaccharide
transporters
within
the
cancer
microenvironment.
One
key
change
alteration
sialic
acid
processing
that
leads
a
general
upregulation
sialylated
glycans
(hypersialylation)
on
cell
surfaces
an
increased
introduction
non-human
N-glycolyl-neuraminic
(Neu5Gc)
instead
N-acetyl-neuraminic
into
surface
glycans.
These
changes
have
been
shown
be
result
sialyltransferase
sialidase
expression.
Functionally,
cancer-associated
hypersialylation
appears
directly
impact
tumor
interaction
microenvironment,
in
particular
modulation
acid-binding
lectins
immune
cells.
Moreover,
Neu5Gc
human
tissues
enhances
inflammation
anti-Neu5Gc
response,
which
can
potentially
influence
inflammation-induced
inflammation.
In
this
review,
we
summarize
biology
microenvironment
resulting
effect
immunity.
Cell,
Journal Year:
2021,
Volume and Issue:
184(19), P. 5031 - 5052.e26
Published: Sept. 1, 2021
Pancreatic
ductal
adenocarcinoma
(PDAC)
is
a
highly
aggressive
cancer
with
poor
patient
survival.
Toward
understanding
the
underlying
molecular
alterations
that
drive
PDAC
oncogenesis,
we
conducted
comprehensive
proteogenomic
analysis
of
140
pancreatic
cancers,
67
normal
adjacent
tissues,
and
9
tissues.
Proteomic,
phosphoproteomic,
glycoproteomic
analyses
were
used
to
characterize
proteins
their
modifications.
In
addition,
whole-genome
sequencing,
whole-exome
methylation,
RNA
sequencing
(RNA-seq),
microRNA
(miRNA-seq)
performed
on
same
tissues
facilitate
an
integrated
determine
impact
genomic
protein
expression,
signaling
pathways,
post-translational
To
ensure
robust
downstream
analyses,
tumor
neoplastic
cellularity
was
assessed
via
multiple
orthogonal
strategies
using
features
verified
pathological
estimation
based
histological
review.
This
characterization
will
serve
as
valuable
resource
for
community,
paving
way
early
detection
identification
novel
therapeutic
targets.
