Genome Report: Pseudomolecule-scale genome assemblies ofDrepanocaryum sewerzowiiandMarmoritis complanata DOI Creative Commons
Samuel J. Smit,

Caragh Whitehead,

Sally James

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 28, 2024

Abstract The Nepetoideae, a subfamily of Lamiaceae (mint family), is rich in aromatic plants, many which are sought after for their use as flavours and fragrances or medicinal properties. Here we present genome assemblies two species Nepetiodeae: Drepanocaruym sewerzowii Marmoritis complanata . Both were generated using Oxford Nanopore Q20+ reads with contigs anchored to nine pseudomolecules that resulted 335 Mb 305 assemblies, respectively, BUSCO scores above 95% both the assembly annotation. We furthermore provide tree only derived gene models, complementing existing transcriptome marker-based phylogenies.

Language: Английский

New insights of advanced biotechnological engineering strategies for tanshinone biosynthesis in Salvia miltiorrhiza DOI

Minyu Shou,

Qinzhe Lin,

Ying Xu

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112384 - 112384

Published: Jan. 1, 2025

Language: Английский

Citations

2
Chromosome-level genome assembly of Salvia sclarea DOI Creative Commons

Sang-Haeng Choi,

Yuna Kang,

Changsoo Kim

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: Jan. 4, 2025

Salvia sclarea is a medicinal herb from the Lamiaceae family, valued for its essential oil which contains sclareol, linalool, linalyl acetate, and other compounds. Despite extensive use, genetic mechanisms of S. are not well understood. This study presents chromosome-level genome assembly using Oxford Nanopore Technology, Illumina short reads, Pore-C technology. The assembled spans 499.03 Mbp with scaffold N50 50.5 Mbp, forming 11 pseudochromosomes. was validated by BUSCO analysis, indicated high completeness marked at 98.7%. 68.73% repetitive sequences, long terminal repeats (LTRs) accounting 33.01%. A total 17,202 protein-coding genes were predicted. Among them, 16,846 annotated in NCBI NR database, highest among all databases, covering 97.93% predicted genes. phylogenetic analysis revealed that shares close evolutionary relationship officinalis within genus, while species genus have evolved independently family. high-resolution offers fresh insights into biosynthesis oils aromatic compounds sclarea, establishing basis preservation.

Language: Английский

Citations

1
The Smi-miR858a-SmMYB module regulates tanshinone and phenolic acid biosynthesis in Salvia miltiorrhiza DOI Creative Commons
Butuo Zhu, Meizhen Wang,

Yongqi Pang

et al.

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(4)

Published: Feb. 23, 2024

Abstract Tanshinones and phenolic acids are two major classes of bioactive compounds in Salvia miltiorrhiza. Revealing the regulatory mechanism their biosynthesis is crucial for quality improvement S. miltiorrhiza medicinal materials. Here we demonstrated that Smi-miR858a–Smi-miR858c, a miRNA family previously known to regulate flavonoid biosynthesis, also played critical roles tanshinone acid Overexpression Smi-miR858a plants caused significant growth retardation reduction. Computational prediction degradome RNA-seq analyses revealed could directly cleave transcripts SmMYB6, SmMYB97, SmMYB111, SmMYB112. Yeast one-hybrid transient transcriptional activity assays showed Smi-miR858a-regulated SmMYBs, such as SmMYB6 SmMYB112, activate expression SmPAL1 SmTAT1 involved SmCPS1 SmKSL1 associated with biosynthesis. In addition activating genes compound pathways, SmMYB112 SmAOC2, SmAOS4, SmJMT2 methyl jasmonate, elicitor plant secondary metabolism. The results suggest existence dual signaling pathways regulation

Language: Английский

Citations

7
Genome-Wide Identification and Functional Analysis of Salvia miltiorrhiza MicroRNAs Reveal the Negative Regulatory Role of Smi-miR159a in Phenolic Acid Biosynthesis DOI Open Access
Hong Zhou,

Maochang Jiang,

Li Jiang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(10), P. 5148 - 5148

Published: May 9, 2024

MicroRNAs (miRNAs) are a group of endogenous small non-coding RNAs in plants. They play critical functions various biological processes during plant growth and development. Salvia miltiorrhiza is well-known traditional Chinese medicinal with significant medicinal, economic, academic values. In order to elucidate the role miRNAs S. miltiorrhiza, six RNA libraries from mature roots, young stems, leaves, leaves flowers one degradome library mixed tissues were constructed. A total 184 miRNA precursors, generating 137 known 49 novel miRNAs, genome-widely identified. The identified predicted diversified regulatory roles plants through regulating 891 genes. qRT-PCR 5′ RLM-RACE assays validated negative smi-miR159a SmMYB62, SmMYB78, SmMYB80. To function bioactive compound biosynthesis, transgenic hairy roots generated analyzed. results showed that overexpression caused decrease rosmarinic acid salvianolic B contents. analysis targets smi-miR159a, including SmMYB80, significantly down-regulated, accompanied by down-regulation SmPAL1, SmC4H1, Sm4CL1, SmTAT1, SmTAT3, SmHPPR1, SmRAS, SmCYP98A14 genes involved phenolic biosynthesis. It suggests regulator biosynthesis miltiorrhiza.

Language: Английский

Citations

6
Systematic characterization of gene families and functional analysis of PvRAS3 and PvRAS4 involved in rosmarinic acid biosynthesis in Prunella vulgaris DOI Creative Commons
Chao Yan, Caili Li,

Maochang Jiang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 1, 2024

Prunella vulgaris is an important material for Chinese medicines with rosmarinic acid (RA) as its index component. Based on the chromosome-level genome assembly we obtained recently, 51 RA biosynthesis-related genes were identified. Sequence feature, gene expression pattern and phylogenetic relationship analyses showed that 17 of them could be involved in biosynthesis. In vitro enzymatic assay PvRAS3 catalyzed condensation p -coumaroyl-CoA caffeoyl-CoA pHPL DHPL. Its affinity toward was higher than caffeoyl-CoA. PvRAS4 lower PvRAS3. UPLC LC-MS/MS existence RA, 4-coumaroyl-3’,4’-dihydroxyphenyllactic acid, 4-coumaroyl-4’-hydroxyphenyllactic caffeoyl-4’-hydroxyphenyllactic P. . Generation analysis pvras3 homozygous mutants significant decrease increase DHPL pHPL. It suggests main enzyme catalyzing acyl donors acceptors during The role appears minor. results provide information quality control medicinal materials.

Language: Английский

Citations

5
Genome-wide identification of the SAUR gene family and screening for SmSAURs involved in root development in Salvia miltiorrhiza DOI
Wei Wang,

Yuwei Zheng,

Lin Qiu

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(7)

Published: June 11, 2024

Language: Английский

Citations

4
Identification of the KNOX Gene Family in Salvia miltiorrhiza Revealing Its Response Characteristics to Salt Stress DOI Creative Commons
Siqi Deng, Wenjing Ma, Chun‐Ping Cui

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 348 - 348

Published: Jan. 24, 2025

Salvia miltiorrhiza is a herbaceous plant that possesses significant medicinal value. Land salinization affects the growth of S. miltiorrhiza, resulting in decline its quality and yield. Knotted1-like homeobox (KNOX) genes are transcription factors play important roles abiotic stress. The characteristics functions KNOX remain unclear. Here, we identified ten all which possess characteristic four domains: KNOX1, KNOX2, ELK, HD. These SmKNOXs were divided into two groups together with homologous genes. Cis-acting element analysis indicated contained elements associated phytohormone, light, stress response. show tissue-specific expression among roots, stems, leaves, flowers. We assessed response to salt using quantitative RT-PCR analysis. Notably, SmKNOX4 significantly decreased within 24 h exposure, while SmKNOX1, SmKNOX2, SmKNOX3, SmKNOX8, SmKNOX9 showed increases. SmKNOX3 was positively correlated their target genes, GA20ox1 S11 MYB. findings suggest respond stress, providing foundation for studies potential genetic improvement miltiorrhiza.

Language: Английский

Citations

0
Ecotype‐specific phenolic acid accumulation and root softness in Salvia miltiorrhiza are driven by environmental and genetic factors DOI Creative Commons
Haomiao Yu, Jinqiu Liao, Yuanyuan Jiang

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 19, 2025

Summary Salvia miltiorrhiza Bunge, a renowned medicinal herb in traditional Chinese medicine, displays distinctive root texture and high phenolic acid content, traits influenced by genetic environmental factors. However, the underlying regulatory networks remain unclear. Here, we performed multi‐omics analyses on ecotypes from four major regions, focusing impacts structure, accumulation lignin composition. Lower temperatures increased UV‐B radiation were associated with elevated rosmarinic (RA) salvianolic B (SAB) levels, particularly Sichuan ecotype. Structural models indicated that radial arrangement of xylem conduits contributes to greater hardness. Genomic assembly comparative analysis ecotype revealed unique metabolism gene cluster, including SmWRKY40 , WRKY transcription factor essential for RA SAB biosynthesis. Overexpression enhanced levels whereas its knockout reduced Integrating high‐throughput (DNA affinity purification sequencing) point‐to‐point (Yeast One‐Hybrid, Dual‐Luciferase Electrophoretic Mobility Shift Assay) protein‐DNA interaction detection platform further identified binding sites across ecotypes, revealing specific networks. Our findings provide insights into molecular basis bioactive compound accumulation, advancing breeding strategies quality improvement S. .

Language: Английский

Citations

0
Genome-wide identification and expression analysis of wall-associated kinases (WAK/WAKLs) in Salvia miltiorrhiza with novel insights into tanshinone regulation in response to fungal elicitors DOI
Ann Abozeid,

Zhongyu Xu,

Kansas Wan

et al.

The Journal of Horticultural Science and Biotechnology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: April 13, 2025

Language: Английский

Citations

0
Genomic research and genetic improvement of orphan crops: novel strategies for addressing global food security challenges DOI
Zijun Wang, Guisheng Xiang

Published: Jan. 1, 2025

Language: Английский

Citations

0