Chinese Science Bulletin (Chinese Version), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 1, 2023
Compendium of plant genomes, Journal Year: 2024, Volume and Issue: unknown, P. 225 - 237
Published: Jan. 1, 2024
Language: Английский
Citations
0
Advances in botanical research, Journal Year: 2024, Volume and Issue: unknown
Published: Jan. 1, 2024
Language: Английский
Citations
0
Horticulture Research, Journal Year: 2024, Volume and Issue: 12(2)
Published: Oct. 15, 2024
Abstract Grasses, including turf and forage, cover most of the earth’s surface; predominantly important for land, water, livestock feed, soil, water conservation, as well carbon sequestration. Improved production quality grasses by modern molecular breeding is gaining more research attention. Recent advances in genome-editing technologies are helping to revolutionize plant also offering smart efficient acceleration on grass improvement. Here, we reviewed all recent researches using (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing tools enhance growth forage grasses. Furthermore, highlighted emerging approaches aimed at advancing program. We assessed CRISPR-Cas effectiveness, discussed challenges associated with its application, explored future perspectives primarily focusing Despite promising potential grasses, current efficiency remains limited due several bottlenecks, such absence comprehensive reference genomes, lack gene delivery tools, unavailability suitable vector species, high polyploidization, multiple homoeoalleles, etc. these challenges, system holds great fully harness benefits genetics, aiming improve sustain quantity
Language: Английский
Citations
0
Forestry Research, Journal Year: 2024, Volume and Issue: 4(1), P. 0 - 0
Published: Jan. 1, 2024
Here, a DNA-free genetic editing approach is presented for larch by delivering ribonucleoprotein complexes (RNPs) of CRISPR/Cas9 through particle bombardment. The detailed procedure encompasses creating transgenic system
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11792 - 11792
Published: Nov. 2, 2024
Forests worldwide are under increasing pressure from climate change and emerging diseases, threatening their vital ecological economic roles. Traditional breeding approaches, while valuable, inherently slow limited by the long generation times existing genetic variation of trees. CRISPR technologies offer a transformative solution, enabling precise efficient genome editing to accelerate development climate-resilient productive forests. This review provides comprehensive overview applications in forestry, exploring its potential for enhancing disease resistance, improving abiotic stress tolerance, modifying wood properties, accelerating growth. We discuss mechanisms various systems, including base editing, prime multiplexing strategies. Additionally, we highlight recent advances overcoming key challenges such as reagent delivery plant regeneration, which crucial successful implementation also delve into ethical considerations using gene drive population-level alterations, well importance containment strategies mitigating risks. emphasizes need continued research, technological advancements, extensive long-term field trials, public engagement, responsible innovation fully harness power shaping sustainable future
Language: Английский
Citations
0
Agriculture, Journal Year: 2024, Volume and Issue: 15(1), P. 29 - 29
Published: Dec. 26, 2024
The creation of the CRISPR–Cas system has provided unprecedented opportunities in plant genome research and crop genetic improvement. In recent years, this been continuously improved to meet human needs through expansion modification Cas proteins, diversification targeting locations, optimization CRISPR vectors. review, we systematically describe Class II proteins that have used plants, deactivated Cas9 (dCas9) its role transcriptional regulation, precision editing systems, protein variants, as well methods examples systems various regions with different breadths. addition, outline plans based on constructs can overcome pleiotropy genes or accelerate generation transgene-free plants applications breeding practices. Finally, discuss theory development “CRISPR plus”, integrated application existing more species.
Language: Английский
Citations
0
Forests, Journal Year: 2023, Volume and Issue: 14(12), P. 2385 - 2385
Published: Dec. 6, 2023
The genus Populus is composed of dioecious woody plants and adult females produce large numbers seed hairs that can affect public health pose a potential fire risk. However, it difficult to distinguish between males based on their morphology at the seedling stage. Therefore, developing technology identifies gender poplar seedlings crucial for controlling hairs. In this study, we developed an approach early identification Tacamahaca Aigeiros species male-specific sequence in simonii. be accurately identified by PCR. sequencing results showed was conserved P. simonii its F1 progenies. Interestingly, there were three nucleobase differences species. Sequence alignment had not been assembled pseudochromosome. Subsequently, fluorescence situ hybridization (FISH) used locate specific short arm end chromosome 19 male This study provides efficient convenient method determination lays groundwork exploring key sex-determination genes.
Language: Английский
Citations
1
Chinese Science Bulletin (Chinese Version), Journal Year: 2023, Volume and Issue: unknown
Published: Dec. 1, 2023
Citations
0