Haplotype‐resolved genome and population genomics provide insights into dioscin biosynthesis and evolutionary history of the medicinal species Dioscorea nipponica

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(3)

Published: Feb. 1, 2025

SUMMARY Dioscorea nipponica , a perennial herb widely distributed in the Sino‐Japanese Floristic Region, is renowned for its medicinal properties, particularly ability to produce dioscin. Here, we present haplotype‐resolved genome assembly of diploid D . comprising 511.41 Mb Haplotype A and 498.29 B, each organized into 10 chromosomes. The two haplotypes exhibited high similarity, with only 2.75% allelic genes exhibiting specific expression. Key dioscin biosynthesis pathway were identified, expression analysis revealed that majority (16/21) involved first stages highly expressed rhizomes. Notably, significant expansion CYP90, CYP94, UGT73 gene families was observed dioscin‐producing species, highlighting their critical roles biosynthesis. Additionally, size estimation population genomic analyses diverse D. accessions identified four principal clades corresponding diploid, di‐tetra‐octoploid, tetraploid, hexaploid from various geographic regions, clade (diploids) further divided five subclades. Demographic prolonged decline effective Pleistocene Last Glacial Maximum, splits occurring during mid‐to‐late Pleistocene. Selective sweep key genes, including HD‐Zip I ADH1 SMT1 CYPs may contribute adaptation high‐latitude environments geographical variations content. Overall, this study enhances our understanding architecture, biosynthetic pathways, evolutionary dynamics providing valuable insights potential evolution.

Language: Английский

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In vitro plant cultures as a viable biotechnological tool for the biosynthesis of steroidal hormones of clinical interest

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 203 - 251

Published: Jan. 1, 2025

Language: Английский

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An HD-ZIP I Transcription Factor DZHDZ32 Upregulates Diosgenin Biosynthesis in Dioscorea zingiberensis

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4185 - 4185

Published: April 28, 2025

Diosgenin, a crucial precursor for steroidal drug production, has poorly understood regulatory pathways. Diosgenin is the primary active component of Dioscorea zingiberensis. Notably, D. zingiberensis also possesses highest diosgenin content among species, reaching up to 16.15% dry weight. This study identified DZHDZ32 as potential regulator biosynthesis in through transient overexpression. To validate its function, we developed an optimized genetic transformation method and generated two DZHDZ32-overexpressing lines. The transcription factor belongs HD-ZIP I subfamily localized nucleus. overexpression resulted significant increase transcript levels leaves (264.59- 666.93-fold), leading elevated biosynthetic intermediates, including cholesterol β-sitosterol. Specifically, increased by 41.68% 68.07%, 10.29% 16.03%, β-sitosterol 12.33% 19.49% compared wild-type plants. Yeast one-hybrid dual-luciferase assays demonstrated that directly binds promoters ACAT GPPS1, consistent with upregulation GPPS1 expression (3.69- 4.87-fold 4.75- 6.53-fold, respectively) overexpressing established key biosynthesis. discovery implications enhancing production advancing development.

Language: Английский

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Transcriptional regulation of polyphyllin biosynthesis of Paris polyphylla in response to soil moisture stress

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 230, P. 121108 - 121108

Published: May 6, 2025

Language: Английский

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