Telomere‐to‐telomere reference genome reveals subgenome divergence and large structural variants in Capsella bursa‐pastoris (Brassicaceae)

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 29, 2025

Abstract Capsella is a model genus for studying polyploid speciation and mating system transitions. Here, we report subgenome‐resolved telomere‐to‐telomere reference genome of bursa‐pastoris , the only species in genus, generated by incorporating PacBio HiFi reads, Nanopore ultra‐long Hi‐C data. The assembled genome, named Cbp_T2T, consisted 16 chromosomes, with 8 each two subgenomes. size final assembly was 330.91 Mb. Multiple evaluation methods confirmed contiguity (contig N50: 20.28 Mb), completeness (Benchmarking Universal Single ‐ Copy Orthologue: 98.70%), accuracy (QV: 35.76) genome. Repetitive sequences accounted 37.04% size. In total, 27,626 28,096 protein‐coding genes were annotated subgenomes, respectively. Large structural variants observed between Additionally, large inversions on chromosomes 6 7, spanning approximately 1.93 2.33 Mb, respectively, detected comparing Cbp_T2T to previously published chromosome‐scale C. assembly. This high‐quality will facilitate evolutionary biological functional genomic studies species.

Language: Английский

Unlocking diversity from wild relatives of perennial fruit crops in the pan-genomics era

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102652 - 102652

Published: Oct. 31, 2024

Language: Английский