A Comparative Transcriptomic Study Reveals Temporal and Genotype-Specific Defense Responses to Botrytis cinerea in Grapevine

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(2), P. 124 - 124

Published: Feb. 7, 2025

Grapevine (Vitis vinifera L.), a globally significant crop, is highly susceptible to Botrytis cinerea, the causative agent of gray mold disease. This study investigates transcriptomic responses B. cinerea in tolerant and grapevine genotypes using RNA sequencing (RNA-seq). Differentially expressed genes (DEGs) were identified at three time points (T1, T2, T3), highlighting both genotype-independent genotype-specific responses. Early-stage infection (T1) revealed rapid robust activation defense pathways genotypes, though genotype showed enhanced modulation metabolic processes by prioritizing secondary metabolism stress adaptation over growth. In contrast, exhibited less coordinated reprogramming, with delayed or weaker key mechanisms. Gene Ontology KEGG analyses critical pathways, including phenylpropanoid biosynthesis-like lignin metabolism, MAPK signaling, as well candidate such WRKY transcription factors enzymes involved cell wall fortification antifungal compound biosynthesis. Genotype-specific emphasized flexibility determinant resistance, exhibiting superior resource allocation pathways. These findings provide insights into molecular basis resistance offering potential targets for breeding genetic engineering enhance resilience reduce fungicide dependency.

Language: Английский

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Distinct Infection Mechanisms of Rhizoctonia solani AG-1 IA and AG-4 HG-I+II in Brachypodium distachyon and Barley

Life, Journal Year: 2025, Volume and Issue: 15(2), P. 235 - 235

Published: Feb. 5, 2025

Rhizoctonia solani is a basidiomycete phytopathogenic fungus that causes rapid necrosis in wide range of crop species, leading to substantial agricultural losses worldwide. The species complex divided into 13 anastomosis groups (AGs) based on hyphal fusion compatibility and further subdivided by culture morphology. While R. classifications were shown be independent host specificity, it remains unclear whether different isolates share similar virulence mechanisms. Here, we investigated the infectivity Japanese Brachypodium distachyon barley. Two isolates, AG-1 IA (from rice) AG-4 HG-I+II cauliflower), infected leaves both plants, but only roots. B. accessions Bd3-1 Gaz-4 barley cultivar ‘Morex’ exhibited enhanced resistance compared Bd21 cultivars ‘Haruna Nijo’ ‘Golden Promise’. During infection, not resistant Morex induced genes for salicylic acid (SA) N-hydroxypipecolic (NHP) biosynthesis. Pretreatment with SA or NHP conferred IA, HG-I+II, susceptible Haruna Nijo. On Nijo, developed extensive mycelial networks numerous infection cushions, which are specialized structures well-characterized rice sheath blight. In contrast, formed dispersed masses associated underlying necrosis. We propose encompasses at least two distinct strategies: exhibits hemibiotrophic lifestyle, while follows predominantly necrotrophic strategy.

Language: Английский

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Proteomic insights into fruit–pathogen interactions: managing biotic stress in fruit

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(3)

Published: Feb. 13, 2025

Language: Английский

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Management Strategies of Grey Mould Disease in Berries

Plant Health Cases, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Abstract Grey mould, caused by the necrotrophic fungus Botrytis cinerea , represents a significant threat to berry crops worldwide, particularly impacting strawberries and raspberries resulting in substantial economic losses annually. This pathogen thrives cool, humid environments, initiating infections on various plant parts, including leaves, flowers, fruits, ultimately leading fruit rot quality degradation. possesses complex disease cycle, overwintering as sclerotia or mycelia producing abundant conidia that spread via wind water. Infection can occur through direct penetration wounds, with symptoms ranging from leaf lesions blossom blight rot. The fungus’s ability adapt develop resistance commonly used chemical fungicides poses major challenge effective management. case study reviews biology, of grey mould berries. It comprehensively examines current management strategies, cultural practices such optimizing air circulation sanitation, controls modes action, increasing importance biological control agents biotechnological approaches. Additionally, this highlights challenges associated fungicide emphasizes need for sustainable integrated strategies minimize ensure high-quality production. Future research directions focus innovative solutions, developing resistant cultivars improving efficacy application biocontrol methods. Information © Authors 2025

Language: Английский

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A Systematic Review on the Advancements in Remote Sensing and Proximity Tools for Grapevine Disease Detection

Sensors, Journal Year: 2024, Volume and Issue: 24(24), P. 8172 - 8172

Published: Dec. 21, 2024

Grapevines (Vitis vinifera L.) are one of the most economically relevant crops worldwide, yet they highly vulnerable to various diseases, causing substantial economic losses for winegrowers. This systematic review evaluates application remote sensing and proximal tools vineyard disease detection, addressing current capabilities, gaps, future directions in sensor-based field monitoring grapevine diseases. The covers 104 studies published between 2008 October 2024, identified through searches Scopus Web Science, conducted on 25 January updated 10 2024. included focused exclusively detection while excluded were not related did use or sensing, conditions. studied diseases include downy mildew, powdery Flavescence dorée, esca complex, rots, viral main sensors RGB, multispectral, hyperspectral sensors, spectroscopy. A trend recent research is integration artificial intelligence techniques, such as machine learning deep learning, improve accuracy. results demonstrate progress monitoring, with concentrating specific sensor platforms, methodological improvements. Future should focus standardizing methodologies, integrating multi-sensor data, validating approaches across diverse contexts commercial applicability sustainability, both environmental challenges.

Language: Английский

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Microbial biological control of Botrytis bunch rot (Botrytis cinerea) of grapevine (Vitis vinifera) crops: a meta-analysis

Crop Protection, Journal Year: 2024, Volume and Issue: unknown, P. 107085 - 107085

Published: Dec. 1, 2024

Language: Английский

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Efficiency of Water Kumquat (Fortunella margarita) Extracts Against Hepatotoxicity Induced by Carbendazim in Vitro and In Vivo

Current Research in Nutrition and Food Science Journal, Journal Year: 2024, Volume and Issue: 12(3), P. 1389 - 1408

Published: Dec. 15, 2024

Objective: The study aims to evaluate the efficiency of water kumquat (Fortunella margarita) extracts in mitigating hepatotoxicity induced by carbendazim (CBZ), a broad-spectrum fungicide, both vitro and vivo. Methods: In vitro, HepG2 liver cancer cells were exposed CBZ determine half-maximal inhibitory concentration (IC50) assess protective effects extracts. vivo, 48 healthy male albino Sprague Dawley rats divided into two main groups: negative control group intoxication group. was further subdivided receive varying concentrations hot or cold Liver functions evaluated through biochemical assays, while histopathological examinations assessed tissue damage. Results: Kumquat exhibited significant hepatoprotective effects. improved cell viability reduced oxidative stress markers compared CBZ-treated cells. treatment with led levels enzymes, antioxidant status, minimized damage CBZ-exposed rats. dose-dependent, higher showing more effectiveness benefits. Conclusion: demonstrate considerable potential protecting against CBZ-induced toxicity. These investigations suggest that could serve as natural therapeutic option for preventing caused pesticide exposure.

Language: Английский

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