The PuERF27-PuMYB10-PuGSTF12 Module Regulates Melatonin-Induced Anthocyanin Biosynthesis in Pear Fruits DOI

Huili Sun,

Xinyue Wang,

Guangfu Gu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Anthocyanins, flavonoids with strong antioxidant activity, give rise to a fruit peel color and provide health benefits. Studies have revealed that melatonin promotes anthocyanin accumulation. However, the function of endogenous hormones in this process remains elusive. Here, we investigated effect ethylene signaling on accumulation melatonin-treated pear fruit. Melatonin promoted while repressing production; its biosynthesis background ethephon pretreatment was eliminated. Transcriptome analysis PuERF27, melatonin-induced response factor (ERF), as candidate gene. Transient overexpression PuERF27 is essential for enhancing activating genes, whereas silencing repressed Yeast one-hybrid, electrophoretic mobility shift, GUS assays indicated could bind directly positively regulate PuMYB10 PuGSTF12 promoters. Further studies together PuMYB10, synergistically transactivates promoter. These results indicate may promote expression indirectly activate structural genes through thereby leading transport anthocyanins.

Language: Английский

p-chlorophenylalanine treatment accelerates tomato fruit ripening through hormone synthesis and glycolytic pathway during storage DOI
Kailin Xu,

Ran Yan,

Hang Zhang

et al.

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 225, P. 113517 - 113517

Published: March 17, 2025

Language: Английский

Citations

0
The PuERF27-PuMYB10-PuGSTF12 Module Regulates Melatonin-Induced Anthocyanin Biosynthesis in Pear Fruits DOI

Huili Sun,

Xinyue Wang,

Guangfu Gu

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 30, 2025

Anthocyanins, flavonoids with strong antioxidant activity, give rise to a fruit peel color and provide health benefits. Studies have revealed that melatonin promotes anthocyanin accumulation. However, the function of endogenous hormones in this process remains elusive. Here, we investigated effect ethylene signaling on accumulation melatonin-treated pear fruit. Melatonin promoted while repressing production; its biosynthesis background ethephon pretreatment was eliminated. Transcriptome analysis PuERF27, melatonin-induced response factor (ERF), as candidate gene. Transient overexpression PuERF27 is essential for enhancing activating genes, whereas silencing repressed Yeast one-hybrid, electrophoretic mobility shift, GUS assays indicated could bind directly positively regulate PuMYB10 PuGSTF12 promoters. Further studies together PuMYB10, synergistically transactivates promoter. These results indicate may promote expression indirectly activate structural genes through thereby leading transport anthocyanins.

Language: Английский

Citations

0