The Small Non-Coding RNA Profile of Human and Mouse Sperm

Non-Coding RNA, Journal Year: 2025, Volume and Issue: 11(1), P. 15 - 15

Published: Feb. 9, 2025

Small non-coding RNAs constitute a dynamic epigenetic layer in mature spermatozoa that can exert transgenerational regulatory functions. Here, we review recent advances the field of small spermatozoa, how their profiles change response to lifestyle or environmental factors, and impact on offsprings' physiology. The profile these changes dramatically during maturation. majority intracellular early spermatogenesis are miRNAs piRNAs, but, tRNA- rRNA-derived fragments (tRFs rRFs, respectively) predominant forms, primarily delivered from epididymis via extracellular vesicles. Diet, exercise, exposures have direct effect RNA levels this differential abundance reprogram development embryo. Offsprings fathers with different lifestyles phenotypes, including altered metabolism behavior. Therefore, emerging as an important inheritance.

Language: Английский

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Epididymal-Born circRNA Cargo and Its Implications in Male Fertility

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2614 - 2614

Published: March 14, 2025

The epididymis represents a pivotal organ for sperm maturation and male fertility maintenance. During the epididymal journey, cells undergo morphological molecular changes that need to acquire morpho-functional skills necessary successful oocyte fertilization. Not last, great enrichment of spermatozoa RNA payload occurs via an epithelium-derived epididymosome transfer. Currently, circular RNAs (circRNAs), class non-coding (ncRNAs), are acquiring prominent role in setting quality parameters. In this regard, they considered potential targets several infertility conditions. Despite their consolidated role, few notions known regarding alleged backsplicing activity. current review, we discuss main aspects along circRNA field reproduction. We also report most recent findings on biogenesis duct, providing new fascinating evidence epididymal-derived circRNAs. Finally, show preliminary compelling data by exploiting experimental mouse model aging. Collectively, these remarkable remodeling shaping its profile maturating spermatozoa.

Language: Английский

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Deciphering the Contribution of Circular RNAs to Age‐Related Decline in Sertoli Cell Survivor

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

ABSTRACT Male fertility declines during aging. This process mainly affects spermatogonia and Sertoli cells, leading to impaired spermatogenesis poor‐quality sperm production. Circular RNAs (circRNAs) are covalently closed RNA molecules produced by backsplicing. In the field of male reproduction, circRNAs boast great potential in regulation morpho‐functional skills. However, their role age‐related reproductive anomalies remains largely elusive. Here, we analyzed phenotype aged mouse experimental model, pointing our attention a putative functional link between cell survival. Our results confirm several testicular defects including: (i) altered morphology seminiferous epithelium; (ii) affected spermatogenesis; (iii) decreased particular, spermatozoa (SPZ) were number association with low motility abnormal (sperm head tail bents). The expression analysis selective spermatic demonstrated de‐regulated profile Aged versus Young SPZ. Among them, turned lens on circAbcb9 as circRNA potentially involved senescence pathway via circRNA/miRNA/mRNA network (ceRNET). Indeed, significant shutdown circAbcb9‐dependent associated prominent increase occurred testis. Interestingly, was also expressed human SPZ at levels men, suggesting conserved role. Collectively, study stimulates greater interest molecular mechanisms behind effect survival, providing new implications for fused protein sarcoma (FUS) sertolian biogenesis.

Language: Английский

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Reproductive toxicology: keeping up with our changing world

Frontiers in Toxicology, Journal Year: 2024, Volume and Issue: 6

Published: Oct. 11, 2024

Reproductive toxicology testing is essential to safeguard public health of current and future generations. Traditional toxicological male reproduction has focused on evaluating substances for acute toxicity the reproductive system, with fertility assessment as a main endpoint infertility adverse outcome. Newer studies in last few decades have significantly widened our understanding what represents an event toxicology, thus changed perspective constitutes toxicant, such endocrine disrupting chemicals that affect offspring intergenerational manner. Besides or congenital abnormalities, outcomes can present increased likelihood various problems offspring, including metabolic syndrome, neurodevelopmental like autism cancer predisposition, among others. To enable toxicologic accurately represent population, designs need model changing population characteristics exposure circumstances. Current trends increasing importance human include paternal age, associated decline nicotinamide adenine dinucleotide (NAD), higher prevalence obesity, both which are factors study design should account for. In this article, we highlighted some limitations standard protocols, expanding assessed by genetic epigenetic sperm parameters, potential recent developments, mixture testing, novel animal models, vitro systems organoids, multigenerational well silico modelling, machine learning artificial intelligence.

Language: Английский

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Circular RNAs as a novel class of potential therapeutic and diagnostic biomarkers in reproductive biology/diseases

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Dec. 31, 2024

Infertility is a prevalent problem among 10% of people within their reproductive years. Sometimes, even advanced treatment options like assisted reproduction technology have the potential to result in failed implantation. Because expected changes gene expression during both vitro and vivo fertilization processes, these methods assisting fertility also been associated with undesirable pregnancy outcomes related infertility. In this aspect, Circular RNAs (circRNAs) play crucial role as epigenetic modifiers wide range biological pathological activities, including problems fertility. CircRNAs are integral pieces multiple cellular functions, moving substances nucleus, silencing one X chromosome, cell death, ability stem cells differentiate into different types, process inherited from parental genes. Due progress made high-speed sequencing, large amount circRNA molecules detected, revealing significant functions diverse enhancing testicular development, preserving differentiation renewal spermatogonial cells, controlling spermatocyte meiosis. Moreover, non-coding contribute aspects female system pregnancy-related diseases, gynecologic cancers, endometriosis. conclusion, there no denying that circRNAs immense be used biomarkers treatments for disorders males females. research, we provide comprehensive analysis women's

Language: Английский

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